Author(s): Payal A. Sheth, Anil T. Pawar, Ganesh B. Choudhari, Chandrakant S. More

Email(s): anil.pawar@mitwpu.edu.in

DOI: 10.52711/0974-360X.2022.00828   

Address: Payal A. Sheth1, Anil T. Pawar1*, Ganesh B. Choudhari1, Chandrakant S. More2
1School of Pharmacy, Dr. Vishwanath Karad World Peace University, Pune, 411038, Maharashtra, India.
2Shri Swami Samarth Ayurvedic Pharmaceuticals, Nashik, 422212, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022


ABSTRACT:
Raktavardhak kadha is a polyherbal formulation available in the Indian market as a nutritional supplement for the treatment of anemia. The present study was conducted to evaluate the phytochemical content and in vitro antioxidant activity of Raktavardhak kadha. A preliminary phytochemical analysis of Raktavardhak kadha was performed to detect the presence of various classes of phytochemicals. The total phenolic and tannin contents were estimated by the Folin-Ciocalteu method. The aluminium chloride colorimetric method was used for the estimation of the total flavonoid content of Raktavardhak kadha. Antioxidant activity was analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method. Phytochemical analysis indicated the existence of saponins, steroids, flavonoids, tannins, and phenolic compounds in the Raktavardhak kadha. The total phenolic content of Raktavardhak kadha was found to be 86.5 mg of gallic acid equivalent per milliliter. The total tannin content of Raktavardhak kadha was found to be 73.33 mg of gallic acid equivalent per milliliter. The total flavonoid content of Raktavardhak kadha was 95.4 mg of quercetin equivalent per milliliter. In the DPPH assay, the IC50 of Raktavardhak kadha was found to be 92.53 ppm. It is concluded that the RK is rich in phytochemical content and has antioxidant properties indicating its high medicinal values. It appears that the presence of flavonoids is responsible for the beneficial effects of Raktavardhak kadha in anemic patients.


Cite this article:
Payal A. Sheth, Anil T. Pawar, Ganesh B. Choudhari, Chandrakant S. More. Phytochemical Content and Antioxidant Property of Polyherbal Formulation, Raktavardhak Kadha. Research Journal of Pharmacy and Technology. 2022; 15(11):4931-4. doi: 10.52711/0974-360X.2022.00828

Cite(Electronic):
Payal A. Sheth, Anil T. Pawar, Ganesh B. Choudhari, Chandrakant S. More. Phytochemical Content and Antioxidant Property of Polyherbal Formulation, Raktavardhak Kadha. Research Journal of Pharmacy and Technology. 2022; 15(11):4931-4. doi: 10.52711/0974-360X.2022.00828   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-11-13


REFERENCES:
1.    Daimari M. Swargiary A. Study of phytochemical content and antioxidant properties of Musa balbisiana corm extract. Indian J Pharm Sci. 2020; 82(4):707-12. doi.org/10.36468/pharmaceutical-sciences.698
2.    Parasuraman S. Thing GS. Dhanaraj SA. Polyherbal formulation: concept of Ayurveda. Pharmacogn Rev. 2014; 8(16):73-80. doi.org/10.4103/0973-7847.134229
3.    Sheth PA. Pawar AT. Mote CS. More C. Antianemic activity of polyherbal formulation, Raktavardhak Kadha, against phenylhydrazine-induced anemia in rats. J Ayurveda Integr Med. 2021; 12(2):340-5. doi.org/10.1016/j.jaim.2021.02.009
4.    Khandelwal KR. Practical Pharmacognosy. 9th edition. New Delhi: Nirali Prakashan; 2002.
5.    Sujatha J. Asokan S. Rajeshkumar S. Phytochemical analysis and antioxidant activity of chloroform extract of Cassis alata. Research J Pharm and Tech. 2018; 11(2):439-44. doi.org/10.5958/0974-360X.2018.00081.1
6.    Rajabpour A. Abdali Mashahdi AR. Ghorbani MR. Chemical compositions of leaf extracts from Conocarpus erectus L. (Combretaceae) and their bioactivities against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). J Asia Pac Entomol. 2019; 22(1):333-7. doi.org/10.1016/j.aspen.2019.01.015
7.    Tiwari P. Phenolics and flavonoids and antioxidant potential of Balarishta prepared by traditional and modern methods. Asian J Pharm Ana. 2014; 4(1):5-10.
8.    Killedar SG. More HN. Seasonal Variation of Tannin Content in Different Parts of Memecylon umbellatum Burm. Research J Pharm and Tech. 2011; 4(1):78-81.
9.    Agbo MO. Uzor PF. Akazine-Nneji UN. Eze-Odurukwe CU. Ogbatue UB. Mbaoji EC. Antioxidant, total phenolic and flavonoid content of selected Nigerian medicinal plants. Dhaka Univ J Pharm Sci. 2015; 14(1):35-41.
10.    Tiwari P. Patel RK. Estimation of total phenolics and flavonoids and antioxidant potential of Ashwagandharishta prepared by traditional and modern methods. Asian J Pharm Ana. 2013; 3(4):147-52.
11.    Shukla A. Kaur A. Shukla RK. Anchal. Comparative evaluation of antioxidant capacity, total flavonoid and phenolic content of Ehretia acuminata R. Br. fruit. Research J Pharm and Tech. 2019; 12(4):1811-6. doi.org/ 10.5958/0974-360X.2019.00302.0  
12.    Gadhave RV. Kuchekar BS. Design, synthesis, characterization and biological evaluation of novel benzothiazole based [1, 2, 4]-triazolo [4, 3-a] quinoxaline derivatives. Indian Drugs. 2019; 56(9):22-9. doi.org/10.53879/id.56.09.11901
13.    Datir SB. Nirmal SA. Ganjare AB. Bhawar SB. Patil MJ. Antioxidant activity of the aerial parts of the Achyranthes aspera Var. Porphyristachya (Wall. Ex Moq.) Hook. F. Research J Pharmacognosy and Phytochemistry. 2009; 1(3):220-3.
14.    Mukhia R. Basistha B. Chhetri DR. Variation in antioxidant activity of a Rattan Species, Plectocomia himalayana Griff. by DPPH assay based on two different methods of methanol extraction. Res J Pharmacognosy and Phytochem. 2018; 10(2):175-8. doi.org/10.5958/0975-4385.2018.00027.4
15.    Datir SB. Patel AM. Patel AK. Patil PP. Rohit FI. Thorat VD. Bharti DK. et al. Evaluation of antioxidant activity of the aerial parts of the Abutilon indicum (Linn) Sweet (Malvaceae). Research J Pharmacology and Pharmacodynamics. 2010; 2(5):303-8.
16.    Pandey MM. Rastogi S. Rawat AKS. Indian traditional Ayurvedic system of medicine and nutritional supplementation. Evid Based Complement Alternat Med. 2013; 2013:376327. doi.org/10.1155/2013/376327
17.    Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000; 63(7):1035-42. doi.org/10.1021/np9904509
18.    Prochazkova D. Bousova I. Wilhelmova N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011; 82(4):513-23. doi.org/10.1016/j.fitote.2011.01.018
19.    Ogbe RJ. Adoga GI. Abu AH. Antianaemic potentials of some plant extracts on phenyl hydrazine-induced anaemia in rabbits. J Med Plant Res. 2010; 4(8):680-4. doi.org/ 10.5897/JMPR09.487
20.    Devasagayam TP. Tilak JC. Boloor KK. Sane KS. Ghaskadbi SS. Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India. 2004; 52:794-804.
21.    Fibach E. Rachmilewitz E. The role of oxidative stress in hemolytic anemia. Curr Mol Med. 2008; 8(7):609-19. doi.org/10.2174/156652408786241384
22.    Joshi H. Pagare M. Patil L. Kadam V. In-vitro antioxidant activity of ethanolic extract of leaves of Buchanania lanzan Spreng. Research J Pharm and Tech. 2011; 4(6):920-4.
23.    Yoo JH. Maeng HY. Sun YK.  Kim YA. Park DW. Park TS. Lee ST. et al. Oxidative status in iron-deficiency anemia. J Clin Lab Anal. 2009; 23(5):319-23. doi.org/10.1002/jcla.20335
24.    Ajibade TO. Oyagbemi AA. Durotoye LA. Omóbòwálé TO, Asenuga ER, Olayemi FO. Modulatory effects of melatonin and vitamin C on oxidative stress-mediated haemolytic anaemia and associated cardiovascular dysfunctions in rats. J Complement Integr Med. 2017; 14(1):20150082. doi.org/10.1515/jcim-2015-0082
25.    Singh AK. Nagpal D. Attrey DP. Characterization of a prepared poly-herbal formulation and comparison of its DPPH scavenging activity with other marketed formulations. Eur J Biol Sci. 2012; 4(3):68-72. doi.org/10.5829/idosi.ejbs.2012.4.3.260
26.    Skrovankova S. Misurcova L. Machu L. Antioxidant activity and protecting health effects of common medicinal plants. Adv Food Nutr Res. 2012; 67:75-139. doi.org/10.1016/B978-0-12-394598-3.00003-4


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