Author(s): Arsyik Ibrahim1, 2*, Siswandono Siswandono3, Bambang Prajogo EW

Email(s): achie.ibrahim@gmail.com

DOI: 10.52711/0974-360X.2022.00796   

Address: Arsyik Ibrahim1,2*, Siswandono Siswandono3, Bambang Prajogo EW3
1Doctoral Program, Faculty of Pharmacy, University of Airlangga, Surabaya 60115, East Java, Indonesia.
2Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Mulawarman, Samarinda 75119, East Kalimantan, Indonesia.
3Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Airlangga, Surabaya 60115, East Java, Indonesia.
3Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Airlangga, Surabaya 60115, East Java, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
Studies of in vitro cytotoxic activity of Sungkai leaf (Peronema canescens Jack) using a human colon and cervical cancer cells have not been reported to date; the report was still limited to bioactivity in seawater shrimp larvae. The leaves of this plant contain many secondary metabolites with potential cytotoxic activity. Plants were a source of natural medicinal ingredients for cancer treatment are known as herbal medicines. Anticancer activity of a natural substance, in vitro cell line assays, can be used. This study aimed to determine the cytotoxicity (IC50) of P. canescens leaf of the chloroform, ethyl acetate, and ethanol extract against HT-29 colon cancer and HeLa cervical cancer cells. The P. canescens leaf was extracted by the maceration method using methanol solvent. The dried sample was separated by the liquid-liquid method and successive polarity gradient eluents: chloroform, ethyl acetate, and ethanol. Cell line to used HT-29 and HeLa cells, Cytotoxicity test with MTT (3-(4,5-dimethyl azole-2-yl)-2,5-diphenyltetrazolium bromide) method. The concentration used was from 1.5µg/mL to 200.0 µg/mL. The cytotoxic activity values (IC50) of each extract on HT-29 and HeLa cells were chloroform (10.353 µg/mL and 38.913µg/mL), ethyl acetate (48.635µg/mL and 28.186µg/mL), and ethanol (42.017µg/mL), and 253.190µg/mL), respectively. The results showed that the cytotoxic activity of chloroform extracts had a strong cytotoxic effect and a moderate effect on HeLa cervical cancer cells. Anticancer research opportunities are wide open for further studies.


Cite this article:
Arsyik Ibrahim1,2*, Siswandono Siswandono3, Bambang Prajogo EW. Anticancer activity of Peronema canescens Jack leaves extracts against human cells: HT-29 and HeLa in vitro. Research Journal of Pharmacy and Technology 2022; 15(10):4739-5. doi: 10.52711/0974-360X.2022.00796

Cite(Electronic):
Arsyik Ibrahim1,2*, Siswandono Siswandono3, Bambang Prajogo EW. Anticancer activity of Peronema canescens Jack leaves extracts against human cells: HT-29 and HeLa in vitro. Research Journal of Pharmacy and Technology 2022; 15(10):4739-5. doi: 10.52711/0974-360X.2022.00796   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-10-69


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