Author(s): Nihad Shahadha Abed, Ergin Kariptaş, Safaa Abed Lateef


DOI: 10.52711/0974-360X.2022.00784   

Address: Nihad Shahadha Abed1, Ergin Kariptaş2, Safaa Abed Lateef3
1Department of Advanced Technologies, Faculty of Science Institute, Kirşehir Ahi Evran Universitesi.: 2Department of Medical Microbiology, Faculty of Medicine, Samsun University, 55080, Samsun, Türkiye.: 3Department of Biotechnology, Faculty of Science, University of Anbar, Anbar, Iraq.: *Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022

Klebsiella pneumoniae is a Gram-negative bacterium that exists naturally in the human body's gastrointestinal tract as part of the natural microflora. It has long been recognized as one of the most frequent nosocomial diseases and a multidrug resistant pathogen. The purpose of the current work was to determine the antimicrobial susceptibility and genetic relatedness of K. pneumoniae strains obtained from Ramadi hospitals, western Iraq, using the ERIC–PCR approach. In the period between June and September 2021, 51 K. pneumonia isolates were obtained from Anbar hospitals. After normal laboratory procedures and differential assays identified K. pneumoniae isolates, disk diffusion susceptibility testing was performed. Also, ERIC-PCR was used to determine genetic relatedness of 15 MDR K. pneumoniae isolates. Kirby –bauer disk diffusion test showed that among K. pneumonia isolates, the highest antibiotic resistance was to Augmentin (96%), cefotaxime (92%), ceftriaxone =Ceftazidime (88%), Amikacin (48%), Levofloxaccin (42%), and imipenem (26%). More than 66.66% of the isolates revealed multi-drug resistant phenotypes. ERIC-PCR results and data analysis indicate that there are 23 distinct ERIC types. The findings of this study show an increase in the prevalence of multiresistant K. pneumoniae in the hospitals studied. The ERIC PCR results revealed a high level of genetic diversity across K. pneumoniae strains, showing that K. pneumoniae isolates are polyclonal in Anbar hospitals.

Cite this article:
Nihad Shahadha Abed, Ergin Kariptaş, Safaa Abed Lateef. Molecular typing of MDR-K. pneumoniae using ERIC-PCR Technique. Research Journal of Pharmacy and Technology 2022; 15(10):4677-0. doi: 10.52711/0974-360X.2022.00784

Nihad Shahadha Abed, Ergin Kariptaş, Safaa Abed Lateef. Molecular typing of MDR-K. pneumoniae using ERIC-PCR Technique. Research Journal of Pharmacy and Technology 2022; 15(10):4677-0. doi: 10.52711/0974-360X.2022.00784   Available on:

1.    Sreeja, M. K., Gowrishankar, N. L., Adisha, S. and Divya, K. C. Antibiotic resistance-reasons and the most common resistant pathogens-A review. Res. J. Pharm. Technol. 10, 1886–1890 (2017). DOI: 10.5958/0974-360X.2017.00331.6
2.    Ahmed, S. H. and Hafidh, R. R. The Isolation of specifically lytic phages along with their extracted endolysins as antibacterial agents to MDR Enterococcus faecalis. Res. J. Pharm. Technol. 2021;14, 4547–4554  . DOI: 10.52711/0974-360X.2021.00791
3.    Parsaie Mehr, V., Shokoohizadeh, L., Mirzaee, M. and Savari, M. Molecular typing of Klebsiella pneumoniae isolates by enterobacterial repetitive intergenic Consensus (ERIC)–PCR. Infect. Epidemiol. Microbiol. 2017 ; 3, 112–116  . URL:
4.    Syntem, L. M. O., Dutta, H., Neelusree, P. and Kalyani, M. Prevalence of extended spectrum ß-Lactamase and Carbapenemase producing isolates of Klebsiella SPP in a tertiary care hospital. Res. J. Pharm. Technol. 2018 ;11, 3777–3780  . DOI : 10.5958/0974-360X.2018.00692.3
5.    Sarshar, S., Mirnejad, R. and Babapour, E. Frequency of blaCTX-M and blaTEM Virulence Genes and Antibiotic Resistance Profiles among Klebsiella pneumoniae Isolates in Urinary Tract Infection (UTI) Samples from Hashtgerd, Iran. Reports Biochem. Mol. Biol. 2021 ; 412–419. doi: 10.52547/rbmb.10.3.412
6.    Kavai, S. M., Kangogo, M., Muigai, A. W. T. and Kariuki, S. Analysis of trends in resistance to fluoroquinolones and extended spectrum beta-lactams among Salmonella Typhi isolates obtained from patients at four outpatient clinics in Nairobi County, Kenya. Adv. Microbiol. 2018 ; 8, 578  .
7.    Shinde, P. S., Mahadik, V. J. and Sarvagod, S. M. Herbal drug standardization and its implication–A current need of time. Res. J. Pharmacogn. Phytochem. 2016 ; 8, 93–100  . DOI : 10.5958/0975-4385.2016.00018.2
8.    Riwu, K. H. P., Effendi, M. H. and Rantam, F. A. A review of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae and multidrug resistant (MDR) on companion animals. Syst Rev Pharm 2020 ; 11, 270–277  . doi = "10.31838/srp.2020.7.43",
9.    Khushboo, K., Saloni, B. and Rathore, K. S. Antibiotic Resistance. Asian J. Res. Pharm. Sci. 2020 ; 10 , DOI : 10.5958/2231-5659.2020.00047.8
10.    Choby, J. E., Howard‐Anderson, J. and Weiss, D. S. Hypervirulent Klebsiella pneumoniae–clinical and molecular perspectives. J. Intern. Med. 2020 ; 287, 283–300  .
11.    Negi, R. K., Rasheed Naikodi, M. A., Sajwan, S., Asim Ali Khan, K. A. S. and Meena, R. P. Standardization and HPTLC Fingerprinting of Unani compound formulation Habb-e-Muqil Jadeed. Alcohol 2021 ; 22, 6  . DOI: 10.5958/0974-4150.2021.00009.2
12.    Jain, T. and Dashora, K. Development of fingerprinting method for Amlakyadi Churna: Spectrophotometric approach. Res. J. Pharmacogn. Phytochem. 2012 ; 4, 61–63  . DOI:
13.    Bobbadi, S. et al. Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products. J. Food Saf. 2020 ; 40, e12738  .
14.    Dhillon, M. S. Pre-History of DNA ‘Fingerprinting’in India. Res. J. Humanit. Soc. Sci. 2019 ; 10, 882–886  . DOI : 10.5958/2321-5828.2019.00145.1
15.    WH, E. Edwards and Ewing’s identification of Enterobacteriaceae. Int J Syst Bacteriol 1986 ; 36, 581–582  . DOI:
16.    Abbey, T. C. and Deak, E. What’s New from the CLSI Subcommittee on Antimicrobial Susceptibility Testing M100. Clin. Microbiol. Newsl. 2019 ; 41, 203–209 .
17.    Bilung, L. M., Pui, C. F., Su’ut, L. and Apun, K. Evaluation of BOX-PCR and ERIC-PCR as molecular typing tools for pathogenic Leptospira. Dis. Markers 2018, .
18.    Clinical And Laboratory Standars Institute. M100 Performance Standards for Antimicrobial Susceptibility Testing. Journal of Services Marketing vol. 2018 ; 8,.
19.    Henshke-Bar-Meir, R. et al. Assessment of the clinical significance of production of extended-spectrum β-lactamases (ESBL) by Enterobacteriaceae. Infection 2006 ; 34, 66–74  .
20.    Cabral, A. B., Melo, R. de C. de A., Maciel, M. A. V. and Lopes, A. C. S. Multidrug resistance genes, including blaKPC and blaCTX-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil. Rev. Soc. Bras. Med. Trop.2012 ; 45, 572–578 .
21.    Mehboob, M. and Shuja, M. N. Isolation, Identification and Characterization of Urinary Tract Infectious Bacteria and the Effect of Different Antibiotics. Biosci. Rev.2021; 3,.
22.    Wei, Z.-Q., Chen, Y.-G., Yu, Y.-S., Lu, W.-X. and Li, L.-J. Nosocomial spread of multi-resistant Klebsiella pneumoniae containing a plasmid encoding multiple β-lactamases. J. Med. Microbiol. 2005 ; 54, 885–888.
23.    Pérez-Moreno, M. O. et al. Molecular epidemiology and resistance mechanisms involved in reduced susceptibility to amoxicillin/clavulanic acid in Klebsiella pneumoniae isolates from a chronic care centre. Int. J. Antimicrob. Agents 2011; 37, 462–466.
24.    Ramazanzadeh, R., Zamani, S. and Zamani, S. Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals. Iran. J. Microbiol. 2013 ; 5, 126 . :
25.    Seifi, K. et al. Evaluation of biofilm formation among Klebsiella pneumoniae isolates and molecular characterization by ERIC-PCR. Jundishapur J. Microbiol. 2013 ; 9,. doi: 10.5812/jjm.30682
26.    Moosavian, M. and Emam, N. The first report of emerging mobilized colistin-resistance (mcr) genes and ERIC-PCR typing in Escherichia coli and Klebsiella pneumoniae clinical isolates in southwest Iran. Infect. Drug Resist.2019; 12, 1001 . doi: 10.2147/IDR.S192597
27.    Edelstein, M., Pimkin, M., Palagin, I., Edelstein, I. and Stratchounski, L. Prevalence and molecular epidemiology of CTX-M extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob. Agents Chemother. 2003; 47, 3724–3732 . DOI:

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