Author(s):
Hany Akeel Al-Hussaniy, Zainab Noori Mohammed, Ali H. Alburghaif, Meena Akeel Naji
Email(s):
hanyakeel2018@gmail.com
DOI:
10.52711/0974-360X.2022.00771 
Address:
Hany Akeel Al-Hussaniy1*, Zainab Noori Mohammed2, Ali H. Alburghaif 3, Meena Akeel Naji4
1PhD Candidate at the Department of Pharmacology College of Medicine, Baghdad University, Iraq.
2Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Iraq.
3Department of Pharmacy, Ashur University Collage, Baghdad Iraq.
4Department of Family Medicine, Collage of Medicine, University of Baghdad, Iraq.
*Corresponding Author
Published In:
Volume - 15,
Issue - 10,
Year - 2022
ABSTRACT:
Background; panax ginseng is one of the best ayurvedic plant used for treatment of several types of disease such as diabetes, improve the libido and hair-lose and as antioxidant also decrease the side effect and toxicity of several chemotherapy and toxic substances and several studies show that it may have an anticancer effect. Aim: to find out the effect of panax ginseng to decrease the cardiotoxicity the occur as a result of several chemotherapeutic medication such as doxorubicin and the effect of panax ginseng on cellular biomarker and cancer suppressor substance for evaluation of possible anticancer effect of panax ginseng. Methods: forty rate module has been enrolled in this study, divided into four groups ten rats for each groups, first group receive purified water, second groups receive Panax ginseng orally in 100mg/kg dose, third group receive panax ginseng with doxorubicin whereas the last groups receive high dose of panax ginseng only, blood sample also collected and organs such as heart is extracted, the serum level of several biomarker and cancer modulators has been evaluated. Results: Panax ginseng significantly reduce cardiotoxicity by its antioxidant mechanism, Panax ginseng reduced cardiac troponin (cTnI) However, its effect on reduction of BNP levels insignificantly compared to the doxorubicin group P=0.06. Panax ginseng reduced LPO and MDA and raised the antioxidant potential biomarker GSH significantly compared to the doxorubicin group P<0.05. Panax ginseng significantly reduced inflammatory (TNF-a) and apoptotic (caspase-3) biomarkers when compared to the doxorubicin group. Panax ginsing increase caspase level also and in addition, MDA, LPO, TNF-a, and caspase-3 levels were increased in doxorubicin group compared to the control group P<0.05. Conclusions: as a result of our research, Panax ginseng significantly show cardioprotective effect that it supresses the oxidative stress and other cardiotoxic parameters , However in high dose show to have anticancer by itself through caspase medullated apoptosis whereas the casepace 3 level significantly
Cite this article:
Hany Akeel Al-Hussaniy, Zainab Noori Mohammed, Ali H. Alburghaif , Meena Akeel Naji. Panax ginseng as Antioxidant and Anti-inflammatory to reduce the Cardiotoxicity of Doxorubicin on rat module. Research Journal of Pharmacy and Technology 2022; 15(10):4594-0. doi: 10.52711/0974-360X.2022.00771
Cite(Electronic):
Hany Akeel Al-Hussaniy, Zainab Noori Mohammed, Ali H. Alburghaif , Meena Akeel Naji. Panax ginseng as Antioxidant and Anti-inflammatory to reduce the Cardiotoxicity of Doxorubicin on rat module. Research Journal of Pharmacy and Technology 2022; 15(10):4594-0. doi: 10.52711/0974-360X.2022.00771 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-10-44
8. REFERENCES:
1. Kim J-H. Pharmacological and medical applications of Panax ginseng and ginsenosides: a review for use in cardiovascular diseases. J Ginseng Res. 2018;42(3):264–9. doi.org/10.1016/j.jgr.2017.10.004
2. Hyun SH, Bhilare KD, In G, Park C-K, Kim J-H. Effects of Panax ginseng and ginsenosides on oxidative stress and cardiovascular diseases: pharmacological and therapeutic roles. J Ginseng Res. 2022;46(1):33–8. doi.org/10.1016/j.jgr.2021.07.007
3. Huang Q, Lou T, Lu J, Wang M, Chen X, Xue L, et al. Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons. J Ginseng Res. 2022; doi.org/10.1016/j.jgr.2022.02.002
4. You L, Cha S, Kim M-Y, Cho JY. Ginsenosides are active ingredients in Panax ginseng with immunomodulatory properties from cellular to organismal levels. J Ginseng Res. 2021; doi.org/10.1016/j.jgr.2021.12.007
5. Wan Y, Wang J, Xu J-F, Tang F, Chen L, Tan Y-Z, et al. Panax ginseng and its ginsenosides: potential candidates for the prevention and treatment of chemotherapy-induced side effects. J Ginseng Res. 2021;45(6):617–30. dx.doi.org/10.1016/j.jgr.2021.03.0011
6. Choi JH, Lee YH, Kwon TW, Ko S-G, Nah S-Y, Cho I-H. Can Panax ginseng help control cytokine storm in COVID-19? J Ginseng Res. 2022;46(3):337–47. doi.org/10.1016/j.jgr.2022.02.006
7. Alkuraishy HM, Al-Gareeb AI, Al-hussaniy HA. Doxorubicin- Induced Cardiotoxicity: Molecular Mechanism and Protection by Conventional Drugs and Natural Products. Int J Clin Oncol Cancer Res 2017; 2:31-44
8. Abdulameer AA, Mohammed ZN, Tawfeeq KT. Endoscopic characteristics and management of Subepithelial Lesions in Video-Gastascopie. Medical and Pharmaceutical Journal. 2022;1(1):4-13. doi.org/10.55940/medphar20222
9. Alkuraishy HM, Al-Gareeb AI. Advances and Prospects of Nicardipine Effects in Attenuation of Hydroxy-Daunorubicin Induced Acute Cardiotoxicity in rats. Adv Biomed Pharma 2015; 2:274-82.
10. Liao L-Y, He Y-F, Li L, Meng H, Dong Y-M, Yi F, et al. A preliminary review of studies on adaptogens: comparison of their bioactivity in TCM with that of ginseng-like herbs used worldwide. Chin Med. 2018;13(1):57. DOI: http://dx.doi.org/10.1186/s13020-018-0214-9
11. Yu C, Fu J, Guo L, Yu M, Yu D. Integrating metabolomics and network pharmacology to explore the protective effect of ginsenoside Re against radiotherapy injury in mice. Evid Based Complement Alternat Med. 2022;2022:5436979. DOI: 10.1155/2022/5436979
12. He B, Chen D, Zhang X, Yang R, Yang Y, Chen P, et al. Oxidative stress and ginsenosides: An update on the molecular mechanisms. Oxid Med Cell Longev. 2022; 2022:9299574. DOI: 10.1155/2022/9299574
13. Xiao D, Yue H, Xiu Y, Sun X, Wang Y, Liu S. Accumulation characteristics and correlation analysis of five ginsenosides with different cultivation ages from different regions. Journal of Ginseng Research. 2015;39(4):338-44. doi.org/10.1016/j.jgr.2015.03.004
14. Liao B, Newmark H, Zhou R. Neuroprotective effects of ginseng total saponin and ginsenosides Rb1 and Rg1 on spinal cord neurons in vitro. Experimental neurology. 2002 1;173(2):224-34. doi.org/10.1006/exnr.2001.7841
15. Zhang F, Wang X, Qiu X, Wang J, Fang H, Wang Z, Sun Y and Xia Z: The protective effect of Esculentoside A on experimental acute liver injury in mice. PLoS One. 9:e1131072014. doi.org/10.1371/journal.pone.0113107
16. Zhuang P, Zhang Y, Pang T. Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica. 2009 ;34(4):443-6.
17. Nam SJ, Han YJ, Lee W, Kang B, Choi MK, Han YH, Song IS. Effect of red ginseng extract on the pharmacokinetics and efficacy of metformin in streptozotocin-induced diabetic rats. Pharmaceutics. 2018;10(3):80. doi.org/10.3390/pharmaceutics10030080
18. Yuan QL, Yang CX, Xu P, Gao XQ, Deng L, Chen P, Sun ZL, Chen QY. Neuroprotective effects of ginsenoside Rb1 on transient cerebral ischemia in rats. Brain research. 2007;1167:1-2. doi.org/10.1016/j.brainres.2007.06.024
19. Kim HJ, Lee SG, Chae IG, Kim MJ, Im NK, Yu MH, et al. Antioxidant effects of fermented red ginseng extracts in streptozotocin- induced diabetic rats. Journal of Ginseng Research. 2011;35(2):129–37.
20. Al-Kuraishy HM, Al-Hussaniy HA, Al-Gareeb AI, Negm WA, El-Kadem AH, Batiha GE, Welson NN, Mostafa-Hedeab G, Qasem AH, Conte-Junior CA. Combination of Panax ginseng CA Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats. Frontiers in Pharmacology. 2022;13. doi.org/10.3389%2Ffphar.2022.905828
21. Chung SI, Kang MY, Lee SC. In vitro and in vivo antioxidant activity of aged ginseng (panax ginseng). Preventive Nutrition and Food Science. 2016;21(1):24–30.
22. Al-hussaniy HA, Altalebi RR, Alburagheef A, Abdul-Amir AG. The Use of PCR for Respiratory Virus Detection on the Diagnosis and Treatment Decision of Respiratory Tract Infections in Iraq. J Pure Appl Microbiol. 2022;16(1):201-6. doi.org/10.22207/JPAM.16.1.10
23. Abdulrazzaq MH. Protective effect of benfotiamine against doxorubicin-induced cardiotoxicity in rabbits. Iraqi Journal of Pharmaceutical Sciences. 2007;16(1):14-7.
24. Al-Hussaniy HA, Alburghaif AH, Naji MA. Leptin hormone and its effectiveness in reproduction, metabolism, immunity, diabetes, hopes and ambitions. Journal of medicine and life; 2021;14(5): 600–5. doi.org/10.25122%2Fjml-2021-0153
25. Meng F, Su X, Li W, Zheng Y. Ginsenoside Rb3 strengthens the hypoglycemic effect through AMPK for inhibition of hepatic gluconeogenesis. Experimental and Therapeutic Medicine. 2017;13(5):2551-7. doi.org/10.3892%2Fetm.2017.4280
26. Lee SH, Hur J, Lee EH, Kim SY. Ginsenoside Rb1 modulates level of monoamine neurotransmitters in mice frontal cortex and cerebellum in response to immobilization stress. Biomolecules and Therapeutics. 2012;20(5):482. dx.doi.org/10.4062/biomolther.2012.20.5.482
27. Al-Hussaniy HA, Al-Kuraishy HM, Abdulameer AG. The Use of Panax Ginseng to Reduce the Cardiotoxicity of Doxorubicin and Study its Effect on Modulating Oxidative Stress, Inflammatory, and Apoptosis Pathways. Open Access Macedonian Journal of Medical Sciences. 2022;10(A):715-9. doi.org/10.3889/oamjms.2022.9479
28. Al-hassany HA, Albu-rghaif AH, Naji M. Tumor diagnosis by genetic markers protein P-53, p16, C-MYC, N-MYC, protein K-Ras, and gene her-2 Neu is this possible. Pakistan Journal of Medical and Health Sciences. 2021;15(8):2350-4. doi.org/10.53350/pjmhs211582350
29. Kale MK, Bhusari KP, Umathe SN. Relationship between the Dynamics of Oxidative Stress and Thyroid State. Research Journal of Pharmacy and Technology. 2008;1(1):14-7.
30. ALZobaidy MA, AlbuRghaif AH, Alhasany HA, Naji MA. Angiotensin-Converting Enzyme Inhibitors May Increase Risk of Severe COVID-19 Infection. Annals of the Romanian Society for Cell Biology. 2021;25(6):17843-9.
31. Liu TP, Liu IM, Cheng JT. Improvement of Insulin Resistance by Panax Ginseng in Fructose − rich Chow − fed RatsT. Hormone and Metabolic Research.2005;37(03):146-51.
32. Al-kuraishy H. M, Al-gareeb Ali I, Naji HA. Febuxostat Modulates Oxidative And Apoptotic Pathways In Acute Doxorubicin Induced Cardiotoxicity: An Experimental Animal Model Study. Asian J Pharm Clin. 2019;12(4):73-76. doi:10.22159/ajpcr.2019.v12i4.31162
33. Balamurugan Gunasekaran, P. Muralidharan, A. Pandiselvi, P. Amutha. Preliminary Phytochemical screening and Anti oxidant activities of Ethanolic extract of Caesalpinia sappan Linn. Research J. Pharm. and Tech. 2008;1(3):179-181
34. Dibyajyoti Saha, Ankit Tamrakar. Xenobiotics, Oxidative Stress, Free Radicals Vs. Antioxidants: Dance Of Death to Heaven’s Life. Asian J. Res. Pharm. Sci. 2011; 1(2):36-38.
35. Kim JH, Yi YS, Kim MY, Cho JY. Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases. Journal of Ginseng Research. 2017 ;41(4):435–43. doi.org/10.1016/j.jgr.2016.08.004
36. Dibyajyoti Saha, Ankit Tamrakar. Xenobiotics, Oxidative Stress, Free Radicals Vs. Antioxidants: Dance Of Death to Heaven’s Life. Asian J. Res. Pharm. Sci. 2011; 1(2):36-38.
37. Ghanshyam B. Jadhav, Ravindranath B. Saudagar. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian J. Res. Pharm. Sci. 2014; 4(2):51-54.
38. Lee SH, Choi KH, Cha KM, Hwang SY, Park UK, Jeong MS, et al. Protective effects of Korean Red Ginseng against sub-acute immobilization stress-induced testicular damage in experimental rats. Journal of Ginseng Research. 2019;43(1):125–34. doi.org/10.1016/j.jgr.2017.09.002
39. Al-hussaniy HA, Altalebi RR, Tylor FM, Alwash AH, Naji MA, Kadhim ZS, Abu-rghaif AR. Leptin Hormone: In Brief. Medical and Pharmaceutical Journal. 2022;1(1):1-3. doi.org/10.55940/medphar20221
40. Chen J, Liu GZ, Sun Q, Zhang F, Liu C yin, Yuan L, et al. Protective effects of ginsenoside Rg3 on TNF-α-induced human nucleus pulposus cells through inhibiting NF-κB signaling pathway. Life Sciences. 2019; 216:1–9. doi.org/10.1016/j.lfs.2018.11.022
41. Ridha DK, Al-Shawi NN. The Impacts of Graded Doses of Pyridoxine on the Biomarkers, Aspartate Aminotransferase, lactate Dehydrogenase and Total Antioxidant Capacity in Doxorubicin-Induced Cardiotoxicity in Female Rats. Iraqi Journal of Pharmaceutical Sciences. 2017:12-21. doi.org/10.31351/vol26iss2pp12-21
42. Merlin NJ, V Parthasarathy, R Manavalan. Role of Flavanoids in Free Radical Induced Hepatic Fibrosis. Research J. Pharm. and Tech. 2009;2(1):52-57.
43. Al-Jammas S, Al-Saraj A. The histological changes induced by Cytarabine on rabbits' livers (with and without vitamin E administration). Iraqi Journal of Veterinary Sciences. 2020;34(1):9-13. doi.org/10.33899/ijvs.2020.163564
44. Al-Kuraishy HM, Al-Gareeb AI, Al-hussaniy HA, Al-Harcan NAH, Alexiou A, Batiha GES. Neutrophil Extracellular Traps (NETs) and Covid-19: A new frontiers for therapeutic modality. International Immunopharmacology. 2022;104. doi.org/10.1016%2Fj.intimp.2021.108516
45. Akeel Naji H. The Psychosocial and Economic Impact of Uveitis in Iraq. RABMS. 2021; 7 (4): 207-215 doi.org/10.52547/rabms.7.4.207
46. Al-hussaniy H, Al-tameemi ZS. Methicillin-Resistant Staphylococcus aureus and New Delhi Metallo beta-lactamases-types of antibiotic resistance, methods of prevention. Med. Pharm J. 2022;1(1):14-24. doi.org/10.55940/medphar20223
47. Shom Prakash Kushwaha, Sunil Kumar Rawat, Pavan Kumar, Abhishek , Kishu Tripathi. Coupling Antioxidant and Antidiabetic assets of 2, 4-Thiazolidinedione Derivatives. Asian J. Pharm. Ana.. 2011; 1(4): 71-73.
48. Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215. DOI: 10.5958/2231-5675.2016.00032.6
49. Muthusamy Senthil Kumar, Srinivasan Balachandran, Shibani Chaudhury. Influence of Incubation Temperatures on Total Phenolic, Flavonoids Content and Free Radical Scavenging Activity of Callus from Heliotropium indicum L. Asian J. Pharm. Res. 2012; 2(4):148-152.
50. Mrunali Potbhare, Deepak Khobragade. In Vitro Evaluation of Antioxidant Potential of Ayurvedic Preparations Lauha Bhasma and Mandura Bhasma. Asian J. Pharm. Res. 2017; 7(2): 63-66.
51. Pattabiraman K., Muthukumaran P. Antidiabetic and Antioxidant Activity of Morinda tinctoria roxb Fruits Extract in Streptozotocin-Induced Diabetic Rats. Asian J. Pharm. Tech. 2011; 1(2):34-39
52. Selvakumar K., Madhan R., Srinivasan G. , Baskar V. Antioxidant Assays in Pharmacological Research. Asian J. Pharm. Tech. 2011; 1(4):99-103.
53. Deepti Dongarwar Inamdar, Afeez M Dinakaran, Vadiraj D Kurdekar, Hemant R Jadhav. Synthesis and Antioxidant Potential of Novel 2-Isoxazole Substituted Chromenones Synthesized by Microwave Irradiation. Asian J. Research Chem. 3(1): Jan.-Mar. 2010; Page 106-109.
54. Patel D.S. , Shah P. B. , Managoli N. B.. Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian J. Pharm. Tech. 2012;2(4):143-147.