Author(s): Nagaraju Kancherla, Anusha. D, Chitra. K, Ravi Babu. K

Email(s): drdanusha@gmail.com

DOI: 10.52711/0974-360X.2022.00767   

Address: Nagaraju Kancherla1, Anusha. D2*, Chitra. K3, Ravi Babu. K4
1Department of Pharmacology, GSL Medical College and General Hospital, Rajamahendravaram, Andhra Pradesh, 533296, (Ph.D) SRIHER- Chennai, India.
2Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, India.
3Department of Pharmaceutical Chemistry, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, India.
4Department of Pharmacology, GSL Medical College and General Hospital, Rajamahendravaram, Andhra Pradesh – 533296, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
The current study was aimed for screening of preliminary phytochemical constituents, estimation of total phenols, flavonoids and evaluation of antioxidant properties of various extracts of Cayratia auriculata (in vitro). Shade dried powder of Cayratia auriculata was exposed to Soxhlet extraction technique with expanding order of extremity of solvents, for example, hexane >chloroform,> ethyl acetate> methanol. Subjective phytochemical screening was finished by standard methods. Total phenolic content was determined by the Folin-Ciocalteau reagent technique using gallic acid as reference standard. Total flavonoid content was determined by the colorimetric technique, utilizing quercetin as standard. In vitro, antioxidant capacity was assessed by utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Phytochemical investigation suggested that the existence of different phytochemical constituents like alkaloids, flavonoids, phenolic mixtures and tannins, proteins, sugars, glycosides, steroids, triterpenoids, and saponins in the all extracts of Cayratia auriculata in varying degree. The methanolic extract showed higher total phenolic 383.99±1.11 mg GAE/g dry extract and total flavonoid content was 64.11±8.89)mg QE/g dry extract when contrasted with different extracts. Methanolic extract demonstrated most elevated Antioxidant activity 96.46±1.97µg/ml when contrasted with different extracts. Among the four extracts of studied plant methanol extract presented the potent free radical scavenging effect with its significantly lower IC50 value (80.66 µg/ml) in comparison with ethyl acetate, chloroform and hexane extracts. The discoveries of the current examination reinforce the expected ability of Cayratia auriculata as a decent option for the examination of novel antioxidant agents for treating physiological disorders.


Cite this article:
Nagaraju Kancherla, Anusha. D, Chitra. K, Ravi Babu. K. Quantitative Estimation of Total Phenols, Flavonoids and Antioxidant Potential of Cayratia auriculata (In-vitro study). Research Journal of Pharmacy and Technology 2022; 15(10):4570-6. doi: 10.52711/0974-360X.2022.00767

Cite(Electronic):
Nagaraju Kancherla, Anusha. D, Chitra. K, Ravi Babu. K. Quantitative Estimation of Total Phenols, Flavonoids and Antioxidant Potential of Cayratia auriculata (In-vitro study). Research Journal of Pharmacy and Technology 2022; 15(10):4570-6. doi: 10.52711/0974-360X.2022.00767   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-10-40


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