Author(s): B. Usharani, R. Monisha

Email(s): raniushab1@gmail.com

DOI: 10.52711/0974-360X.2022.00009   

Address: B. Usharani*, R. Monisha
Department of Biochemistry, School of Life Sciences, VISTAS, Chennai, 600117, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 1,     Year - 2022


ABSTRACT:
HeLa cells are broadly used for in-vitro studies in bringing out the medicinal value of plants against some dreadful diseases. From time immemorial, it is well proven that plant extracts play a vital role in treating many diseases including cancer. The plant extract contains copious varied phenolic compounds and flavonoids that are majorly responsible for its medicinal properties. In our study we have focused on the extraction of betel quid (betel leaves, areca nut and slaked lime in an appropriate proportion) using the solvent hexane. The hexane extract of the betel quid was evaluated for in vitro studies of apoptosis of HeLa cells. The results revealed that hexane extract of the betel quid at the maximum concentration of 200 µg/mL was very effective and only 20.89 % of cells were viable. The cytotoxic effect against HeLa cells was significant with IC50 of 96.33µg/ml. Further studies can be done on concentrating the bioactive compounds responsible for the anticancer activity. The bioactive active compounds can be formulated in the treatment of cancer in the upcoming research.


Cite this article:
B. Usharani, R. Monisha. In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells. Research Journal of Pharmacy and Technology. 2022; 15(1):47-0. doi: 10.52711/0974-360X.2022.00009

Cite(Electronic):
B. Usharani, R. Monisha. In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells. Research Journal of Pharmacy and Technology. 2022; 15(1):47-0. doi: 10.52711/0974-360X.2022.00009   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-1-9


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