Author(s): Irma Erika Herawati, Jutti Levita, Ronny Lesmana, Anas Subarnas


DOI: 10.52711/0974-360X.2022.00067   

Address: Irma Erika Herawati1,2*, Jutti Levita1, Ronny Lesmana3,4, Anas Subarnas1
1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, West Java, Indonesia 45363.
2Department of Pharmacy, Universitas Al Ghifari, West Java, Indonesia 40293.
3Physiology Molecular Laboratory, Central Laboratory, Universitas Padjadjaran, West Java, Indonesia 45363.
4Department of Medical Basic Sciences, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia 45363.
*Corresponding Author

Published In:   Volume - 15,      Issue - 1,     Year - 2022

Ribosome-inactivating proteins (RIPs) are toxins that are capable to permanently inhibit the synthesis of protein. RIP type II is a heterodimeric protein consisting of an A subunit linked to a lectin-like B chain by a disulfide bridge. Ricin contained in the seeds of Ricinus communis L. is a type II RIP which possesses cytotoxicity activity. However, due to the non-selectivity of the B-lectin chain, ricin needs delivering-vehicles to enhance its cytotoxicity. This article is devoted to reviewing the anticancer activity of ricin in castor bean seeds (a plant type II RIPs) and its various types of cytotoxicity enhancers.

Cite this article:
Irma Erika Herawati, Jutti Levita, Ronny Lesmana, Anas Subarnas. Ricin in Castor Bean (Ricinus communis L.) Seeds: A Review on its Anticancer Activity and The Role of Cytotoxicity Enhancers. Research Journal of Pharmacy and Technology. 2022; 15(1):405-8. doi: 10.52711/0974-360X.2022.00067

Irma Erika Herawati, Jutti Levita, Ronny Lesmana, Anas Subarnas. Ricin in Castor Bean (Ricinus communis L.) Seeds: A Review on its Anticancer Activity and The Role of Cytotoxicity Enhancers. Research Journal of Pharmacy and Technology. 2022; 15(1):405-8. doi: 10.52711/0974-360X.2022.00067   Available on:

1.    Virgilio M, Lombardi A, Caliandro R, Fabbrini MS. Ribosome-inactivating proteins: From plant defense to tumor attack. Toxins. 2010; 2: 2699-2737.
2.    Zhou Y, Li XP, Kahn JN, Tumer NE. Functional assays for measuring the catalytic activity of ribosome-inactivating proteins. Toxins. 2018; 10(6): 240.
3.    Zhu F, Zhou YK, Ji ZL, Chen XR. The plant ribosome-inactivating proteins play important roles in defense against pathogens and insect pest attacks. Frontiers in Plant Sciences. 2018; 9:146.
4.    Sharma N, Park SW, Vepachedu R, Barbieri L, Ciani M, Stirpe F, Savary BJ, Vivanco JM. Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity. Plant Physiology. 2004; 134: 171-181.
5.    Sampada S. Sawant, Vishal R. Randive, Savita R. Kulkarni. Lectins from seeds of Abrus precatorius: Evaluation of Antidiabetic and Antihyperlipidemic Potential in Diabetic Rats. Asian J. Pharm. Res. 2017; 7(2):71-80.
6.    Grela P, Szajwaj M, Horbowicz-Drożdżal P, Tchórzewski M. How ricin damages the ribosome. Toxins. 2019; 11(5): 241.
7.    Hendrix DK, Brenner SE, Holbrook SR. RNA structural motifs: building blocks of a modular biomolecule. Quarterly Reviews of Biophysics. 2005; 38(3): 221–243.
8.    Shi X, Khade PK, Sanbonmatsu KY, Joseph S. Functional role of the sarcin–ricin loop of the 23S rRNA in the elongation cycle of protein synthesis. Journal of Molecular Biology. 2012; 419(3-4): 125-138.
9.    Voorhees RM, Schmeing TM, Kelley AC, Ramakrishnan V. The mechanism for activation of GTP hydrolysis on the ribosome. Science. 2010; 330: 835.
10.    Szewczak AA, Moore PB, Chang YL, Wool IG. The conformation of the sarcin/ricin loop from 28S ribosomal RNA. Proceedings of the National Academy of Sciences of the USA. 1993; 90:  9581-9585.
11.    Walsh MJ, Dodd JE, Hautbergue GM. Ribosome-inactivating proteins. Potent poisons and molecular tools. Virulence. 2013; 4(8): 774–784.
12.    Jena J , Gupta AK. Ricinus communis a phytopharmacological review, International Journal of Pharmacy Pharmaceutical Sciences 2012; 4(4): 25-29.
13.    Rutenber E, Katzin BJ, Ernst  S, Collins EJ, Misna D, Ready MP, Robertus JD. Crystallographic refinement of ricin to 2.5 A. Proteins. 1991. 10: 240-250.
14.    Li XP, Kahn PC, Kahn JN, Grela P, Tumer NE. Arginine residues on the opposite side of the active site stimulate the catalysis of ribosome depurination by ricin A chain by interacting with the P-protein stalk. The Journal of Biological Chemistry. 2013; 288(42):30270-30284.
15.    Chandrasekar. R, Sivagami. B, M. Niranjan Babu. A Pharmacoeconomic Focus on Medicinal Plants with Anticancer Activity. Res. J. Pharmacognosy and Phytochem. 2018; 10(1): 91-100.
16.    Krishnasamy L, Selvam MM, Ravikrishnan B. Anticancer property of Colchicine isolated from Indigofera aspalathoids. Research J. Pharm. and Tech. 2016; 9(4): 386-390.
17.    Merlin NJ, Parthasarathy V, Manavalan R, Devi P, Meera R. Apoptosis Significance and Molecular Mechanisms- A Review. Asian J. Research Chem. 2009. 2(4): 369-375.
18.    Mukesh KN, Satish P, Rajnikant P, Shikha S, Sanjay JD, Manju RS, Deependra S. Lung Cancer Targeting: A Review. Research J. Pharm. and Tech. 2013. 6(11): 1302-1306.
19.    Manoj SP, Hardik J, Leena P, Vilasrao JK. Human Milk: Excellent Anticancer Alternative. Research J. Pharm. and Tech. 2012. 5(1): 14-19.   
20.    Namrata D, Bhavna D, Skand M, Yogeshwer S. Lupeol Induced Apoptosis in Human Lung Cancer Cell Line: A Flow Cytometry Study. Research Journal of Pharmacology and Pharmacodynamics. 2014; 6(4): 197-203
21.    Epy ML, Widjiati, Maslichah M, Bambang PSR, Eka PH. Crosstalk between Necrosis and Apoptosis of Embryonal Cerebral Cortex Neuron Mice (Mus musculus) Caused by Carbofuran Exposure. Research J. Pharm. and Tech. 2019; 12(11): 5492-5498.
22.    Diksha, SP. Role of Stem Cells in treatment of different Diseases. Research J. Pharm. and Tech 2018; 11(8): 3667-3678.
23.    Hendrian DS, Susy F, Paulus S, Bambang P, Ugroseno YB, Endang RK. Detection of the Calcium and ATP Role in Apoptosis of Retinoblastoma Culture Cells through Caspase-3 Expression. Research J. Pharm. and Tech. 2019; 12(3): 1307-1314.
24.    Amanjot K, Mahendra PS. A Mini Review on Environmental Contaminant induced Programmed cell Death in Fruit Fly. Research J. Pharm. and Tech. 2019; 12(12): 6174-6180.
25.    Rao PV, Jayaraj R, Bhaskar AS, Kumar O, Bhattacharya R, Saxena P, Dash PK, Vijayaraghavan R. Mechanism of ricin-induced apoptosis in human cervical cancer cells. Biochemical Pharmacology 2005; 69(5): 855-865.  
26.    Jetzt AE, Cheng J, Nilgun E, Tumer NE, Cohick WS. Ricin A-chain requires c-Jun N-terminal kinase to induce apoptosis in nontransformed epithelial cells. The International Journal of Biochemistry and Cell Biology. 2009; 41(12): 2503–2510.
27.    Bharadwaj S, Rathore SS, Ghosh PC. Enhancement of the cytotoxicity of liposomal ricin by the carboxylic ionophore monensin and the lysosomotropic amine NH4Cl in Chinese hamster ovary cells. International Journal of Toxicology. 2006; 25(5):349-59.
28.    Nguyen LTB, Ngo NT, Nguyen TLN, Nguyen DT. Anticancer Activities of Ricin-Liposome Complexes on SKMEL-28 Cells. Asian Pacific Journal of Cancer Prevention. 2019; 20(7): 2117–2123.
29.    Tyagi N, Rathore SS, Ghosh PC. Efficacy of Liposomal Monensin on the Enhancement of the Antitumour Activity of Liposomal Ricin in Human Epidermoid Carcinoma (KB) Cells. Indian Journal of Pharmaceutical Sciences 2013; 75(1): 16–22.
30.    Tyagi N, Rathore SS, Ghosh PC. Enhanced killing of human epidermoid carcinoma (KB) cells by treatment with ricin encapsulated into sterically stabilized liposomes in combination with monensin. Drug Delivery. 2011; 18(6): 394-404.
31.    Tyagi N, Ghosh PC. Folate receptor mediated targeted delivery of ricin entrapped into sterically stabilized liposomes to human epidermoid carcinoma (KB) cells: effect of monensin intercalated into folate-tagged liposomes. European Journal of Pharmaceutical Sciences. 2011; 43(4): 343-353.
32.    Li C, Yan R, Yang Z, Wang H, Zhang R, Chen H, Wang J. BCMab1-Ra, a novel immunotoxin that BCMab1 antibody coupled to Ricin A chain, can eliminate bladder tumor. Oncotarget. 2017; 8(28): 46704–46705.

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