Author(s): Noushin Anjum, Md. Jamal Hossain, Fahima Aktar, Mohammad Rashedul Haque, Mohammad Abdur Rashid, Md. Ruhul Kuddus

Email(s): ruhulkuddus@du.ac.bd

DOI: 10.52711/0974-360X.2022.00019   

Address: Noushin Anjum1, Md. Jamal Hossain1,2, Fahima Aktar1, Mohammad Rashedul Haque1, Mohammad Abdur Rashid1, Md. Ruhul Kuddus1*
1Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh.
2Department of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka-1205, Bangladesh.
*Corresponding Author

Published In:   Volume - 15,      Issue - 1,     Year - 2022


ABSTRACT:
People in Bangladeshi village area have long practice to take plant-based products for their basic health care. Schleichera oleosa (Lour.) Oken (Family: Sapindaceae) is an important folk medicine in Bangladesh, India that has been used to cure a wide variety of human ailments. Here, the crude methanol extract of S. oleosa leaf (MESOL) and its various solvent (Hexane, dichloromethane, ethyl acetate, aqueous) fractions were evaluated to determine the level of biological activities by both In vitro and in vivo approaches. The crude methanol extract along with its different solvent fractions was investigated for antioxidant activity by measuring total phenolic content and DPPH radical scavenging assay. Cytotoxic activity was performed by brine shrimp lethality bioassay method. The blood clot lysis ability was screened using aspirin as standard. In vitro anti-inflammatory test was performed by RBC membrane stabilizing activity. Beside In vitro analysis, tail immersion procedure and formalin-induced writhing test were carried out to evaluate the analgesic activity of the plant extract in mice. In addition, the anti-diarrheal activity was determined by castor oil-induced diarrheal model in mice. The ethyl acetate fraction of S. oleosa showed prominent antioxidant activity by scavenging DPPH radical with an IC50 value of 9.46 µg/ml, possibly due to its highest phenol content (103.23 mg of GAE/g of plant extract). The crude methanol extract revealed significant cytotoxicity towards brine shrimp with an LC50 value of 16.79 µg/ml. The dichloromethane fraction showed moderate blood clot lysis ability (28.93% clot lysis) while the crude methanol extract of S. oleosa leaf produced the highest 74.62% inhibition of hemolysis that was induced by hypotonic solution. During in vivo assay, the crude methanol extract of S. oleosa leaf produced significant (p<0.05) and dose-dependent pain response and anti-diarrheal effect in mice. The present study revealed that Schleichera oleosa possesses significant pharmacological activities. However, additional studies are compulsory to discover the mechanism of action of this plant extract.


Cite this article:
Noushin Anjum, Md. Jamal Hossain, Fahima Aktar, Mohammad Rashedul Haque, Mohammad Abdur Rashid, Md. Ruhul Kuddus. Potential In vitro and In vivo Bioactivities of Schleichera oleosa (Lour.) Oken: A Traditionally Important Medicinal Plant of Bangladesh. Research Journal of Pharmacy and Technology. 2022; 15(1):113-1. doi: 10.52711/0974-360X.2022.00019

Cite(Electronic):
Noushin Anjum, Md. Jamal Hossain, Fahima Aktar, Mohammad Rashedul Haque, Mohammad Abdur Rashid, Md. Ruhul Kuddus. Potential In vitro and In vivo Bioactivities of Schleichera oleosa (Lour.) Oken: A Traditionally Important Medicinal Plant of Bangladesh. Research Journal of Pharmacy and Technology. 2022; 15(1):113-1. doi: 10.52711/0974-360X.2022.00019   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-1-19


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