Author(s): Meiriza Djohari, Enda Mora, Emrizal, Cici Angraini, Fakhriyah Aulia

Email(s): meirizadj@gmail.com

DOI: 10.52711/0974-360X.2022.00013   

Address: Meiriza Djohari*, Enda Mora, Emrizal, Cici Angraini, Fakhriyah Aulia
Sekolah Tinggi Ilmu Farmasi Riau, Jalan Kamboja, Simpang Baru Panam, 28292.
*Corresponding Author

Published In:   Volume - 15,      Issue - 1,     Year - 2022


ABSTRACT:
Hypertriglyceridemia is condition when there is an increase of triglyceride levels in blood. Hypertriglyceridemia can be increased blood pressure and heart problems. One of the plants can be used as a medicine is kasturi citrus peel (Citrus japonica Thunb.). This study aimed to determine the effect of giving butano fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) to triglyceride levels on male white rats (Rattus norvegicus). The parameters are triglyceride levels in serum of male white rat induced with high-fat feed containing quail egg yolk (10 ml/kgWB). This research used 25 male white rats that divided into 5 groups with 5 rats per group. Normal groups are not given any treatment, only standard feed. The negative group was induced by high-fat feed and suspension Na CMC, the positive group was given high-fat feed and atorvastatin suspension 0,9 mg/kgWB and treatment group was given high-fat feed induction and suspension of ethyl acetate and butanol fraction of kasturi citrus peel with doses 250 mg/kgBW, as much 1% of body weight wich given orally. The triglyceride levels are calculating by using colorimetri enzymatic method with a photometer Mindray® (BA-88A) at wavelength 510 nm. The data percentage change in triglyceride levels on day 29 to day 15 obtained were analyzed with one-way Analysis of Variance (ANOVA) and continued with the Post Hoc Tukey test for each fraction. The results showed that giving butanol fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) at doses of 250 mg/kg body weight had an effect on the decrease in triglyceride levels in serum of white male rat indicated by a significant difference (p<0.05) with negative control and showed a decrease in triglyceride levels activity that was not significant difference (p>0.05) with atorvastatin and there is no significant difference between anti hypertriglyceride activity of butanol fraction and ethyl acetate fraction (p>0.05)


Cite this article:
Meiriza Djohari, Enda Mora, Emrizal, Cici Angraini, Fakhriyah Aulia. Ethyl Acetate and Butanol Fraction Effect of Citrus japonica Thunb. On Triglyceride Levels In Serum White Male Rats (Rattus norvegicus). Research Journal of Pharmacy and Technology. 2022; 15(1):71-6. doi: 10.52711/0974-360X.2022.00013

Cite(Electronic):
Meiriza Djohari, Enda Mora, Emrizal, Cici Angraini, Fakhriyah Aulia. Ethyl Acetate and Butanol Fraction Effect of Citrus japonica Thunb. On Triglyceride Levels In Serum White Male Rats (Rattus norvegicus). Research Journal of Pharmacy and Technology. 2022; 15(1):71-6. doi: 10.52711/0974-360X.2022.00013   Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-1-13


REFERENCES :
1.    Hall, E. J.  Guyton and Hall Textbook of Medical Physiology. 2016. 13th ed. p863-874
2.    Kementrian Kesehatan Republik Indonesia. Laporan Hasil Riset Kesehatan Dasar. Balitbangkes. 2019
3.    Mayes, A,P, and Botham, K.M. Harper’s illustrated Biochemistry. 2015. 30th ed. p211
4.    Brunton, L. Goodman and Gillman’s The Pharmacological basis of Therapeutics. 2011. 12th ed. p877
5.    Bhat, R., Nor, S.B.T., Liong, Min-Tze., and Karim, A.A. Sanication Improves Kasturi Lime (Citrus microcarpa) Juice Quality. Ultrasonics Sonochemistry. 2011.18(6) : 1295-1300.
6.    Wang, Y,C., Chuang, Y,C., Hsu, H,W. The Flavonoid, Carotenoid and Pectin Content in Peels of Citrus Cultivated in Taiwan. Food Chemistry. 2008.106(1), 277-284.
7.    Kurowska, E. M., Spence, J. D., Jordan, J., Wetmore, S., Freeman, D. J., Piché, L. A.,  Serratore, P. HDL-cholesterol-raising effect of orange juice in subjects with Hypercholesterolemia. Journal of Clinical Nutrition. 2000. 36(11)  : 1095-1099.
8.    Surlitah, S., Setiawan, B., Briawan, D. Perbaikan Profil Lipid Pada Perempuan Dewasa Kelebihan Berat Badan Setelah Intervensi Sari Jeruk Kalamansi (citrus microcarpa). J. Gizi Pangan.  2017.12(2), 93-100.
9.    Sandriyana. Pengaruh Pemberian Ekstrak Etanol Kulit Buah Jeruk Kasturi (Citrus japonica Thunb.) Terhadap Kadar Trigliserida Serum Tikus Putih  (Rattus norvegicus) Jantan. Skripsi. Fakultas Farmasi Sekolah tinggi Ilmu Farmasi Riau. 2018.
10.    Moore, D.M. Laboratory Animal Medicine and Science. Edisi 2. Health Science Education Resources. Amerika Serikat. 2000.
11.    Camelia, S., dan Sima, A.. Statins : Mechanism of Action and Effects. Journal Celllular Mol. Med. 2001. 5(4) : 378-387.
12.    Kusuma, A.M., Genatrika, E., Utami, R.F.N., Rohmiyati, T., Susanti,. Efek Ekstrak Bawang Dayak (Eleutherine palmifolia (L)Merr) dan Ubi Ungu (Ipomoea batatus L) terhadap Penurunan Kadar Kolesterol dan Trigliserida Darah pada Tikus Jantan. Jurnal Kefarmasian Indonesia. 2016.108-116.
13.    Budiyono, W. Perbedaan Kadar Kolesterol Total dan Trigliserida Sebelum dan Setelah Pemberian Sari Daun Cincau Hijau (Premna oblongifolia Merr.) pada Tikus Dislipidemia. Skripsi. Fakultas Kedokteran Universitas Diponegoro.  2012.
14.    Sirois, M. Laboratory Animal Medicine. United of State America Mosby Inc. 2005. p87-115.
15.    Gropper, S. S., Smith, J. Groff J. L. Advance Nutrition and Human Metabolism 5th Edition. USA. Wadsworth. 2009. p143-146
16.    Anwar, T. M., Interrelation of Saturated Fat, Trans Fat, Alcohol Intake and subclinical Atherosclerosis. Journal Clinical Nutrition. 2008.16(3):168-174
17.    Murray RK, Graaanner DK, Mayes PA, Rodwell VM. Biokimia Harper. terjemahan oleh Alexander dan A.Hartono; EGC: Jakarta. 2003.
18.     Olivera, T., Ricardo, K., Almeida, M.R., Costa, M.R., Nagem, T.J. Hipolipidemic Effect of Flavonoids and Cholestyramine in Rats Tania. Latin American Journal of Pharmacy. 2007.26(3) : 407-411.
19.    Elzbieta, J. Molace, V., Ragusa, S. Molecular Mechanism of Lipid and Glucose Lowering Activities of Flavonoids. Article in Pharma Nutrition. 2016. 5(5) : 29-58.
20.    Ricardo, K. F. S., Oleiviera, T. T. D., Nagem, T. J., Pinto, A. D. S., Almeida, D. M., Soares, J. S. Effect of Flavonoids Morin; Quercetin and Nicotinic Acid on Lipid Metabolism of Rats Experimentally Fed With Triton. Brazilian Archives of Biology and Technology an International Journal. 2001.44(3) : 263-267
21.    Hsu, C.L., and Yen, G.C.. Effects of Flavonoids and Phenolic Acids on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes.  journal Of Agric and Food Chem. 2007 55(21) : 8404-8410
22.     Lunagariya, N.A., Partel, N.K., Jagtap, C dan Bhutani, K.K. Inhibitors of Pancreatic Lipase. state of the Art and clinical perspectives. Experimental and Clinical sciences Journal. 2014.13:897-921.
23.    Warditiani, N.K., Indrani, A.A.I.S., Sari, N.A.P.P., Swasti, I.A.S., Dewi, N.P.A.K., Widjaja, I.N.K., Wirasuta, I.M.A.G. Pengaruh Pemberian Fraksi Terpenoid Daun Katuk ( Sauropus androgynus (L.) Merr) Terhadap Profil Lipid Tikus Putih (Rattus norvegicus, L) Jantan Galur Wistar yang diinduksi Pakan Kaya Lemak.  jurnal Farmasi Udayana. 2015.

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