Sanaa K. Gomaa, Rania A. Zaki, Reham R. El-Behery, Heba A. El-Refai
Sanaa K. Gomaa1, Rania A. Zaki1, Reham R. El-Behery2, Heba A. El-Refai1
1Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Cairo, 12622, Egypt.
2National Center for Radiation Research and Technology.
Volume - 15,
Issue - 1,
Year - 2022
Green synthesis of MgO nanoparticles (MgO NP) is a facile method that can be easily used for various biomedical, agricultural, catalysis, physics, chemistry, and electronic fields. The construction of nanoparticles by this method makes it compatible with antibacterial studies. Statistical analysis was employed to derive a statistical model for the individual effect of the physicochemical conditions affecting the production conditions of MgO NP to upturn the dry weight to 3 mg /50 ml, which is 6.66 times larger than a basal condition (0.45 mg /50ml). The biosynthesized MgO NP was characterized using UV-VIS, FT-IR, XRD, and TEM. The green synthesized MgO NP killed both gram positive and gram-negative bacteria and disrupted nascent biofilms thus could be used as a potential nanomaterial for in vivo applications such as coating for a medical implant, suggesting new antimicrobial mechanisms. Apart from this, the work reported here stands out from others in various aspects. First, this is the first report (to the best of our knowledge), which calculates the yield of synthesized MgO NP by Bacillus paramycoides strain MCCC 1A04098. Secondly, conditions for optimal yield, different particle sizes of MgO NP were screened using Plackett Burman design and confirmed by UV-VIS spectroscopy, FT-IR, and XRD.
Cite this article:
Sanaa K. Gomaa, Rania A. Zaki, Reham R. El-Behery, Heba A. El-Refai. Eco-Friendly Biogenic Synthesis of Metallic MgO Nanoparticles as Potent Antibacterial and Antibiofilm. Research Journal of Pharmacy and Technology. 2022; 15(1):63-0. doi: 10.52711/0974-360X.2022.00012
Sanaa K. Gomaa, Rania A. Zaki, Reham R. El-Behery, Heba A. El-Refai. Eco-Friendly Biogenic Synthesis of Metallic MgO Nanoparticles as Potent Antibacterial and Antibiofilm. Research Journal of Pharmacy and Technology. 2022; 15(1):63-0. doi: 10.52711/0974-360X.2022.00012 Available on: https://rjptonline.org/AbstractView.aspx?PID=2022-15-1-12
1. Ketan B P, Narendra BP, Sushmita V P, Vaishnavi K P and Pratik C S. Metal based Nanomaterial’s (Silver and Gold): Synthesis and Biomedical application. Asian Journal of Pharmacy and Technology. 2020; 10(2): 97-106.
2. Vignesh BE and Tamil S A. Nanopharmacology: A Novel Approach in Therapeutics. Asian Journal of Research in Pharmaceutical Sciences. 2019; 9(1): 9-16.
3. Ajay S, Naveen C J and Monika R. Magnesium oxide Nanoparticles (MgO NPs): Green Synthesis, Characterizations and Antimicrobial activity. Research Journal Pharmacy and Technology.2019; 12(10):4644-4646.
4. Lyubchenko YL and Shlyakhtenko LS. Visualization of supercoiled DNA with atomic force microscopy in situ. Proceedings of the National Academy of Sciences of the United States of America. 1997; 94: 496-501.
5. Wagner E, Plank C, Zatloukal K, Cotton M and Birnstiel L. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene transfer vehicle. Proceedings of the National Academy of Sciences of the United States of America. 1992; 89: 7934- 7938.
6. Katz A, Alimova A, Min X, Rudolph E and Shah M K. Bacteria size determination by elastic light scattering. IEEE Journal of Selected Topics in Quantum.2003; 9: 277-287.
7. Shobha G, Moses V and Ananda S. Biological synthesis of copper nanoparticles and its impact. International Journal of Applied Pharmaceutics. 2014; 3: 28-38.
8. Khan I, Ibrahim A A M, Sohail M and Qurashi A. Sonochemical assisted synthesis of RGO/ZnO nanowire arrays for photoelectrochemical water splitting. Ultrasonics sonochemistry. 2017; 37: 669-675.
9. Luechinger N A, Grass R N, Athanassiou E K and Stark W J. Bottom-up fabrication of metal/metal nanocomposites from nanopart. Chemistry of Materials. 2010; 22(1):155–160.
10. Manohar DK, Amit A J, Suraj B K and Rahul P J. A Review on Nanoparticles and its Application. Asian Journal of Pharmacy and Technology. 2019; 9(2) : 115-124.
11. Reni M L. Toxic Effect of Nanoparticles of Metals (Pb, Cd, Ag, Mn, Fe and Zn) and Metal Oxides (ZnO, CuO, TiO2 and CeO2) in Human Body. Asian Journal of Research in Chemistry.2013; 6(12) : 1179-1182.
12. Sathish S K, Melchias G, Ravikumar P, Chandrasekar R and Kumaravel P. Bioinspired synthesis of silver nanoparticles using Euphorbia hirta leaf extracts and their antibacterial activity. Asian Journal of Pharmaceutical Research.2014;4(1):39-43.
13. Hisham A Abbas. Antibacterial, Anti-swarming and Antibiofilm Activities of Local. Egyptian Clover Honey Against Proteus Mirabilis Isolated from Diabetic Foot Infection. Asian Journal of Pharmaceutical Research. 2013; 3(3) : 114-117.
14. Baskar G, Chandhuru J, Sheraz Fahad K and Praveen A S. Mycological Synthesis, Characterization and Antifungal Activity of Zinc Oxide Nanoparticles. Asian Journal of Pharmacy and Technology. 2013; 3(4):142-146.
15. Aabasaheb B. T, Prakash D J, Mayur N G, Ashwinkumar S C and Smita P B. A Review on Nanoparticles. Asian Journal of Pharmacy and Technology.2020; 10(2):118-120.
16. Xiangqian L, Huizhong X, Zhe-Sheng C and Guofang C. Biosynthesis of Nanoparticles by Microorganisms and Their Applications. Journal of Nanomaterials.2011; 2011: 1- 16.
17. Malik A. Metal Bioremediation through Growing Cells. Environment International. 2004; 30: 261-278.
18. Ghashghaei S and Emtiazi G. The methods of nanoparticle synthesis using bacteria as biological nanofactories, their mechanisms, and major applications. Current Bionanotechnology.2015; 1: 3-17.
19. Irena M. Microbial synthesis of metal nanoparticles, in Rai M and Nelson D.(Eds.) Metal Nanoparticles in Microbiology, Springer Science & Business Media. pp. 153-175 (2011)
20. Gesser H D and Goswami P C. Aerogels, and Related Porous Materials. Chemical Reviews. 1989: 488-765.
21. Sharma G, Soni R and Dut Jasuja N. Phytoassisted synthesis of magnesium oxide nanoparticles with Swertiachirayaita. Journal of Taibah University for Science. 2017; 11: 471-477.
22. Hussein H Al-S and Esam J Al-K. Antimicrobial and antivirulence activity of magnesium oxide nanoparticles synthesized using klebsiella pneumonia culture filtrate. Biochemical and Cellular Archives.2020; 20:1-12.
23. Imani M M and Safaei M. Optimized Synthesis of Magnesium Oxide Nanoparticles as Bactericidal Agents. Journal of Nanotechnology.2019; 2019: 1-7.
24. Tiwari D K,Behari J and Sen P. Time and dose dependent antimicrobial potential of Ag nanoparticles synthesized by top-down approach. Current Science. 2008; 95: 647– 655.
25. Vidic J, Stankic S, Haque F, Ciric D, Le Goffic R,Vidy A, Jupille J and Delmas B. Selective antibacterial effects of mixed ZnMgO nanoparticles. Journal of nanoparticle research.. 2013; 15:1595 -1605.
26. Barabadi H, Honary S, Ebrahimi P, Mohammadi M A, Alizadeh A and Naghibi F. Microbial mediated preparation, characterization and optimization of gold nanoparticles. Brazilian Journal of Microbiology.2014; 45: 493-501.
27. Bajaj I B and Singhal R S. Enhanced production of poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by using metabolic precursors. Applied Biochemistry and Biotechnology. 2009; 159: 133-141.
28. Bodour A A, Drees K Pand Raina M M. Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid Southwestern soils. Applied and environmental microbiology. 2003; 69: 3280–3287.
29. El-Batal A I, Gharib F A E-L, Ghazi S M. Physiological responses of two varieties of common bean (Phaseolus vulgaris L.) to foliar application of silver nanoparticles. Nonmaterial and Nanotechnology.2016; 6: 1-13.
30. Plackett R L and Burman J P. The Design of Optimum Multi factorial Experiments. Biometrika.1946; 33: 305–325.
31. Yu X, Hallett S G, Sheppard J and Watson A. K. Application of the Plackett-Burman experimental design to evaluate nutritional requirements for the production of Colletotrichum coccodes spores. Applied Microbiology and Biotechnology. 1997; 47: 301-305.
32. Pretorius J C, Magama S and Zietsman P C. Growth inhibition of plant pathogenic bacteria and fungi by extracts from selected South African plant species. South African Journal of Botany. 2003; 20: 188–192.
33. Suryawanshi M V, Waghmode S R, Bharti N, Choudhari P B and Hingamire T B. Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratiamarcescens OX_ R strain. Journal of Applied Pharmaceutical Science. 2016; 11: 52-58.
34. Christensen G D, Simpson WA, Bisno A L and Beachey E H. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infection and Immunity.1982; 37: 318–326.
35. Mathur T, Singhal S, Khan S, Upadhyay D J, Fatma T and Rattan A, Detection of biofilm formation among the clinical isolates of staphylococci: An evaluation of three different screening methods. Indian Journal of Medical Microbiology. 2006; 24: 25-29.
36. Gonzalo J, Serna R, Sol´is J, Babonneau D and Afonso C N. Morphological and interaction effects on the surface plasmon resonance of metal nanoparticles. Journal of Physics : Condensed Matter. 2003;15: 3001–3010.
37. Altschul S F, Madden T L, Schäffer A A, Zhang J, Zhang Z, Miller W and Lipman D J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. 1997; 25: 1-15.
38. Tamura K, Dudley J, Nei M and Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution. 2007; 24: 1596-1605.
39. El-Sayyad G S, Mosallam F M, El-Batal A I. One-pot green synthesis of magnesium oxide nanoparticles using Penicilliumchrysogenum melanin pigment and gamma rays with antimicrobial activity against multidrug-resistant microbes. Advanced Powder Technology.2018; 29: 2616-2625.
40. Thi Nguyen N-Y, Grelling N,Wetteland C L, Rosario R and Liu H. Antimicrobial Activities and Mechanisms of Magnesium Oxide Nanoparticles (nMgO) against Pathogenic Bacteria, Yeasts, and Biofilms. Scientific Reports. 2018; 8: 16260 -16283.
41. Huang L, Li D Q, Lin Y J, Evans D G and Duan X. Influence of nano-MgO particle size on bactericidal action against Bacillus subtilis var. niger. Chinese Science Bulletin. 2005; 50: 514-519.
42. LiaoY Y, Strayer-Scherer A, White J C, De La Torre-Roche R, Ritchie L, Colee J, Vallad G E J, Freeman J, Jones J Band Paret M L. Particle-size dependent bactericidal activity of magnesium oxide against Xanthomonas perforans and bacterial spot of tomato. Scientific Reports.2019; 9:18530-18540.
43. Feldheim D L and Foss C A (Eds.) Metal Nanoparticles: Synthesis, Characterization and Applications, Marcel Dekker Inc, New York and Basel, New York and Basel, (2002).
44. Dobrucka R and Dlugaszewska J. Antimicrobial activity of the biogenically synthesized core-shell Cu@ Pt nanoparticles. Saudi Pharmaceutical Journal. 2018; 26: 643-650.
45. Khan I, Saeed K and Khan I. Nanoparticles: Properties, applications and toxicities. Arabian Journal of Chemistry.2019; 12: 908-931.
46. EL-Moslamy H S, Shokry H H, Rezk H A and Abdel-Fattah R Y. Bioprocess Strategies and Characterization of Anti- Multidrug Resistant Human Pathogens Copper/Copper Oxide Nanoparticles from Citrus Peel Waste Extracts. Journal of Nanomaterials & Molecular Nanotechnology. 2017; 6(1):1-14.
47. Gajengi L A, Sasaki T and Bhanage B M. Mechanistic aspects of formation of MgO nanoparticles under microwave irradiation and its catalytic application. Advanced Powder Technology. 2017; 28: 1185-1192.
48. Sundrarajan M, Suresh J and Gandhi R R. A comparative study on antibacterial properties of MgO nanoparticles prepared under different calcination temperature. Digest Journal of Nanomaterials and Biostructures. 2012; 7: 983-989.
49. Espitia P J P, Soares N F F, Coimbra J S R, Andrade N J, Cruz R S and Medreiros E A A. Zinc oxide nanoparticles: Synthesis, antimicrobial activity and food packaging applications. Food Bioprocess Technology. 2012; 5: 1447-1464.
50. Yim G, Wang H H and Davies J. The truth about antibiotics. International Journal of Medical Microbiology. 2006; 296: 163-170.
51. Rita N N R. Bactericidal and Anti-biofilm Formation of Aqueous Plant Extracts against Pathogenic Bacteria. Asian Journal of Pharmaceutical Research. 2017; 7(1): 25-29.