Author(s): Krutika Patel, Sudheer Kumar Verriboina, S.G. Vasantharaju

Email(s): sg.vasanthraj@manipal.edu

DOI: 10.52711/0974-360X.2021.00876   

Address: Krutika Patel, Sudheer Kumar Verriboina, S.G. Vasantharaju*
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal - 576 104, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021


ABSTRACT:
A simple, accurate, specific and stability-indicating RP-HPLC method was developed for simultaneous determination of chlorzoxazone, diclofenac sodium and paracetamol, using C18 Vydac Monomeric 120A (250 × 4.6mm, 5µ) at 40ºC. The mobile phase contains a mixture of 20mM potassium dihydrogen phosphate buffer (pH 6.2 adjusted with potassium hydroxide) and acetonitrile (30:70 v/v). The flow rate was 1ml/min and detection was carried out at 275nm using PDA detector. The retention time of paracetamol, chlorzoxazone and diclofenac sodium were 3.28mins, 13.27mins and 15.61mins respectively. The analytical curve was linear over a concentration range of 0.65- 6.5µg/ml for paracetamol, 1-10µg/ml for chlorzoxazone and 0.1-1µg/ml for diclofenac sodium. The drugs in bulk and tablet were subjected to acid and alkali hydrolysis, oxidation, thermal and photolytic degradation. This method can be successfully employed for simultaneous quantitative analysis of Chlorzoxazone, Diclofenac sodium and Paracetamol in bulk drug and tablet formulation.


Cite this article:
Krutika Patel, Sudheer Kumar Verriboina, S.G. Vasantharaju. Stability Indicating Assay Method Development and Validation of Simultaneous Estimation of Chlorzoxazone, Diclofenac Sodium and Paracetamol in Bulk Drug and Tablet by RP-HPLC. Research J. Pharm. and Tech. 2021; 14(9):5024-8. doi: 10.52711/0974-360X.2021.00876

Cite(Electronic):
Krutika Patel, Sudheer Kumar Verriboina, S.G. Vasantharaju. Stability Indicating Assay Method Development and Validation of Simultaneous Estimation of Chlorzoxazone, Diclofenac Sodium and Paracetamol in Bulk Drug and Tablet by RP-HPLC. Research J. Pharm. and Tech. 2021; 14(9):5024-8. doi: 10.52711/0974-360X.2021.00876   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-90


REFERENCES:
1.    Pawar UD, Naik AV, Sulebhavikar AV, Datar TA, Mangaonkar KM. Simultaneous Determination of Aceclofenac, Paracetamol and Chlorzoxazone by HPLC in Tablet Dose Form. E-Journal of Chemistry, 2009; 6(1): 289-294.
2.    Badgujar MA, Pingale SG, Mangaonkar KV. Simultaneous Determination of Paracetamol, Chlorzoxazone and Diclofenac Sodium in Tablet Dosage Form by High Performance Liquid Chromatography. E-Journal of Chemistry, 2011; 8(3): 1206-1211
3.    Chaudhari BG, Prajapati HR. Stability-Indicating RP-HPLC Method for Simultaneous Estimation of Chlorzoxazone and Paracetamol in Tablet Dosage Form. Int. J. Pharm. Anal. 2014; 2(5): 402-412
4.    Patel SA, Prajapati KM. Development and Validation Of RP-HPLC Method for Simultaneous Estimation Of Chlorzoxazone And Diclofenac Sodium In Combination. Pharmatutor. Epub. Pharmatutor-Art-1715
5.    Kulkarni MB, Dange PB, Walode SG. Stability indicating thin-layer chromatographic determination of chlorzoxazone, diclofenac sodium and paracetamol as bulk drug: Application to forced degradation study. Der Pharmacia Sinica 2012; 3(6): 643-652

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