Prawati Nuraini, Mega Moeharyono Puteri, EksaArinda Pramesty
Prawati Nuraini1*, Mega Moeharyono Puteri1, EksaArinda Pramesty2
1Assistance Professor of Pediatric Dentistry Department, Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia.
2Student Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia.
Volume - 14,
Issue - 9,
Year - 2021
Dental caries is a disease caused by Streptococcus mutans. The use of chlorhexidine to inhibit bacterial colonization has side effects such as tooth staining and can kill the normal flora when used long term. Epigallocatechin gallate (EGCG) is a chemical compound in the form of polyphenols from green tea catechins which have antimicrobial potency to inhibit microorganism growth and biofilm formation. Type Laboratory Experimental Research In-vitro. The group that will be studied are the negative control group in the form of S.mutans + 5% sucrose, the treatment group in the form of S. mutans + 5% sucrose and EGCG concentration of 0.125mg/ml, 0.25mg/ml, 0.375mg/ml and a positive control group is S.mutans + 5% sucrose and 0.1% chlorhexidine. Data were analyzed using the Kolmogorov-Smirnov test to determine the normality of the data, Levene's test for homogeneity of data, One Way ANOVA Post Hoc Tukey HSD Multiple Comparison to determine differences between treatments. Results: There were significant differences between the treatment groups and the negative control at test results Post Hoc Tukey HSD and the significant differences in the concentration of EGCG 0.375mg/ml with the positive control given chlorhexidine 0.1% (p <0.05). Epigallocatechin gallate (EGCG) influence on the activity of S. mutans biofilm formation and EGCG concentration of 0.375mg/ml are more effective as an antibiofilm of S. mutans compared with chlorhexidine 0.1%.
Cite this article:
Prawati Nuraini, Mega Moeharyono Puteri, EksaArinda Pramesty. Anti-biofilm Activity of Epigallocatechin gallate (EGCG) against Streptococcus mutans bacteria. Research Journal of Pharmacy and Technology. 2021; 14(9):5019-3. doi: 10.52711/0974-360X.2021.00875
Prawati Nuraini, Mega Moeharyono Puteri, EksaArinda Pramesty. Anti-biofilm Activity of Epigallocatechin gallate (EGCG) against Streptococcus mutans bacteria. Research Journal of Pharmacy and Technology. 2021; 14(9):5019-3. doi: 10.52711/0974-360X.2021.00875 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-89
1. Lamont, RJ., Hajishengallis, GN., Jekinson, HF. Oral Microbiology and Immunology third Edition. ASM Press. Washington DC. 2019; 229-230. [internet] http:// asmscience.org
2. Basic Health Research in the Ministry of Health (MoH). Jakarta: Agency for Health Research and Development MOH. 2018; 197-207.
3. Nakano, M. Role of Streptococcus mutans Surface Proteins for Biofilm Formation. Japanese Dental Science Review. 2018; 54(1): 22-29.
4. Jamal, M., Ahmad, W., Andleeb, S., Jalil, F., Imran, M. Nawaz, MA, Kamil, MA Bacterial Biofilms and Associated Infections. Journal of the Chinese Medical Association. 2018; 81(1): 7-11.
5. Singh, S., Singh, SK, Chowdhury, I., and Singh, R. Understanding the Mechanism of Bacterial Resistance to Antimicrobial Agents biofilms. The Open Microbiology Journal. 2017; 11(1): 53-62.
6. Wang, H., and Ren, D. Controlling Streptococcus mutans and Staphylococcus aureus biofilms with direct current and chlorhexidine. AMB Express. 2015; 7(1): 204.
7. World Health Organization. WHO Global Report on Traditional and Complementary Medicine. World Health Organization. 2019
8. Guo, YJ, Zhang, B., Feng, XS, Ren, HX, and Xu, JR Human cathelicidin LL-37 Enhance the antibiofilm effect of EGCG on Streptococcus mutans. BMC oral health. 2016; 16(1): 101.
9. Reygaert, WC The antimicrobial possibilities of green tea. Frontiers in Microbiology. 2018; 5(AUG): 1-8
10. Xu, X., Zhou, XD, and Wu, CD The tea catechin epigallocatechin gallate suppresses cariogenic virulence factors of Streptococcus mutans. Antimicrobial Agents and Chemotherapy. 2011; 55(3): 1229-1236.
11. Roy, R., Tiwari, M., Donelli, G., and Tiwari, V. Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence. 2018; 9(1): 522-554.
12. Vidigal, PG, Musken, M., Becker, KA, Haussler, S., Wingender, J., Steinmann, E., Steinmann, J. Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm. PLoS ONE. 2014; 9(4): 1-8.
13. Eng, Q., Thanikachalam, P. and Ramamurthy, S. Molecular understanding of epigallocatechin gallate (EGCG) in cardiovascular and metabolic disease. Journal of Ethnopharmacology. 2018; 210: 296-310.
14. Sutton, S. Measurement of microbial cells by optical density. Validation Journal Techn. 2011; 17(1): 46-49.
15. Yadav, K., and Prakash, S. Dental caries: A Microbiological Approach. Journal of Clinical Infectious Diseases and Practice. 2017; 02(01): 1-15.