Gajanand Nangare, Varsha Tegeli, Suyash Ingle, Vinod Matole, Avinash Birajdar, Sagar Adlinge
Gajanand Nangare*, Dr. Varsha Tegeli, Suyash Ingle, Vinod Matole, Avinash Birajdar, Sagar Adlinge
D.S.T.S. Mandal’s College of Pharmacy, Solapur - 413004 Maharashtra, India.
Volume - 14,
Issue - 9,
Year - 2021
Objectives: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Dapsone in pure form and Semisolid Dosage form. Method: This UV method was developed using methanol: RO water (75:25) as a solvent. In the present method the wavelength selected for analysis was 260nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 3-18µg/ml. Accuracy was performed by using recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The %RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of Dapsone in bulk and Semisolid dosage form. Hence the present UV spectroscopic method is suitable for routine assay of Dapsone in bulk and Semisolid dosage form.
Cite this article:
Gajanand Nangare, Varsha Tegeli, Suyash Ingle, Vinod Matole, Avinash Birajdar, Sagar Adlinge. UV Spectrophotometric Analysis and Validation of Dapsone in Semisolid Dosage Form. Research Journal of Pharmacy and Technology. 2021; 14(9):5007-9. doi: 10.52711/0974-360X.2021.00872
Gajanand Nangare, Varsha Tegeli, Suyash Ingle, Vinod Matole, Avinash Birajdar, Sagar Adlinge. UV Spectrophotometric Analysis and Validation of Dapsone in Semisolid Dosage Form. Research Journal of Pharmacy and Technology. 2021; 14(9):5007-9. doi: 10.52711/0974-360X.2021.00872 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-86
1. Mahore J, Suryawanshi S, Shirolkar S, Deshkar S. Enhancement of Percutaneous Delivery of Dapsone by Microemulsion Gel, J Young Pharm 2007; 9(4): 507-512.
2. Seshadri V, Manohari P, Kunchithapatham N. Formulation and evaluation of Dapsone topical gel 7.5% w/w, World J Pharm. Research 2018; 7(5): 1281-1299.
3. Indian Pharmacopoeia 2007; (3): 376.
4. Williams D, Lemke T. Foye’s Principles of Medicinal Chemistry, Philadelphia: Lippincott Williams and Wilkins, Baltimore: Wolters and Kulwer Business 2013; 7(7): 1192.
6. Rohini Wankhade, Suhasini Bhalerao et al. Analysis of erythromycin and benzoyl peroxide in combined dosage form by UV-Visible shectrophotometry. Int J Pharm Pharm Sci.2012; 4(4): 527-531
7. Kraingkrai Ponhong, Kate Grudpan. A rapid and sensitive spectrophotometric method for the determination of benzoyl peroxide in wheat flour samples. J of Food and Drug Analysis 2015; 23(4): 652-659.
8. Harikishor Barange, Suhail Ashar and Ujami Sheikh. Development of analytical method for simultaneous estimation of Adapalene and Benzoyl peroxide in gel formulation by RP-HPLC. Global J of Pharmacy and Pharmaceutical Sciences 2018; 4(3): 555639.
9. P.T. Tanner, A.Y.S Wong. Spectrophotometric determination of hydrogen peroxide in rainwater. Analytica Chimica Acta 1998; 370(3): 279-287.
10. Ankush Gupta, Monica Gulati and Narendra kumar Pandey. A validated UV spectrophotometric method for simultaneous estimation of tretinoin and benzoyl peroxide in bulk and semisolid dosage form. Rasayan J. Chem.2009; 3(2): 649-654.
11. Samang Supharoek, Kate Grudpan. A green analytical method for benzoyl peroxide determination by a sequential injection spectrophotometry using natural reagent extract from pumpkin. Talanta 2017; 171: 236-241.
12. ICH, Q2 (R1) Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization; Nov.1996.