V. Hemalatha, T. Silambarasan, R. Dhandapani
V. Hemalatha1, T. Silambarasan1,2, R. Dhandapani1*
1Fermentation Technology Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Salem, Tamil Nadu, India.
2School of Allied Health Sciences, VIMS Hospital Campus, Vinayaka Missions Research Foundation (Deemed to be University), Salem, Tamil Nadu, India.
Volume - 14,
Issue - 9,
Year - 2021
In the present study, Sulfated polysaccharides were extracted from macroalga Sargassum whitey collected from the Gulf of Mannar, Tamil Nadu, India. The maximum yield of 1.95g sulfated polysaccharide was extracted from Sargassum whitey and 0.81mg/ml of purified sulfated polysaccharide was obtained through DEAE anion exchange chromatography. The purified form was characterized using Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray spectroscopy (EDX), and Field Emission Scanning Electron Microscopy (FESEM) analyses. The in vitro biological properties such as antibacterial, antioxidants activity (DPPH, Hydroxyl & Superoxide anion) of sulfated polysaccharides were evaluated and the results showed that 45µg mL-1 concentration of sulfated polysaccharide exhibited good antibacterial inhibitory activity against all the tested pathogenic bacteria strains, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. The in vitro antioxidant activity also found to be significant, where the exhibited IC50 concentrations by the purified sulfated polysaccharide was 183.2µg/mL for DPPH, 204.2µg/mL for superoxide anion, and 163.8µg/mL for Hydroxyl scavenge free radicals activities.
Cite this article:
V. Hemalatha, T. Silambarasan, R. Dhandapani. Extraction, Purification, Characterization and In Vitro Antibacterial properties of Sulfated Polysaccharide Extracted using Sargassum sp. Research Journal of Pharmacy and Technology. 2021; 14(9):4991-8. doi: 10.52711/0974-360X.2021.00869
V. Hemalatha, T. Silambarasan, R. Dhandapani. Extraction, Purification, Characterization and In Vitro Antibacterial properties of Sulfated Polysaccharide Extracted using Sargassum sp. Research Journal of Pharmacy and Technology. 2021; 14(9):4991-8. doi: 10.52711/0974-360X.2021.00869 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-83
1. Doi RH, Kosugi A. Cellulosomes: plant-cell-wall-degrading enzyme complexes. Nature Reviews Microbiology. 2004; 2(7): 541-51.
2. Andriani Y, Syamsumir DF, Yee TC, Harisson FS, Herng GM, Abdullah SA, Orosco CA, Ali AM, Latip J, Kikuzaki H, Mohamad H. Biological Activities of Isolated Compounds from Three Edible Malaysian Red Seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp. Natural Product Communications. 2016; 11(8):1934578X1601100822.
3. Li X, Wang J, Zhang H, Zhang Q. Renoprotective effect of low-molecular-weight sulfated polysaccharide from the seaweed Laminaria japonica on glycerol-induced acute kidney injury in rats. International Journal of Biological Macromolecules. 2017; 95: 132-7.
4. Bouhlal R, Haslin C, Chermann JC, Colliec-Jouault S, Sinquin C, Simon G, Cerantola S, Riadi H, Bourgougnon N. Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales). Marine Drugs. 2011; 9(7): 1187-209.
5. Melo FR, Pereira MS, Foguel D, Mourão PA. Antithrombin-mediated anticoagulant activity of sulfated polysaccharides different mechanisms for heparin and sulfated galactans. Journal of Biological Chemistry. 2004; 279(20): 20824-35.
6. Becker CF, Guimarães JA, Mourão PA, Verli H. Conformation of sulfated galactan and sulfated fucan in aqueous solutions: Implications to their anticoagulant activities. Journal of Molecular Graphics and Modelling. 2007; 26(1): 391-9.
7. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976; 72(2): 248-54.
8. Yemm EW, Willis A. The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal. 1954 Jul 1; 57(3): 508-14.
9. Chou HJ, Kuo JT, Lin ES. Comparative Antioxidant Properties of Water Extracts from Different Parts of Beefsteak Plant (Perilla frutescens). Journal of Food & Drug Analysis. 2009: 17(6).
10. Othman MN, Hassan R, Harith MN, Sah AS. Red seaweed Gracilaria arcuata in cage culture area of Lawas, Sarawak. Borneo Journal of Resource Science and Technology. 2015; 5(2): 53-61
11. Saunders GW. Routine DNA barcoding of Canadian Gracilariales (Rhodophyta) reveals the invasive species Gracilaria vermiculophylla in British Columbia. Molecular ecology resources. 2009; 9: 140-50.
12. Song SL, Lim PE, Phang SM, Lee WW, Lewmanomont K, Largo DB, Han NA. Microsatellite markers from expressed sequence tags (ESTs) of seaweeds in differentiating various Gracilaria species. Journal of Applied Phycology. 2013; 25(3): 839-46.
13. Oyesiku OO, Egunyomi A. Identification and chemical studies of pelagic masses of Sargassum natans (Linnaeus) Gaillon and S. fluitans (Borgessen) Borgesen (brown algae), found offshore in Ondo State, Nigeria. African Journal of Biotechnology. 2014; 13(10).
14. Camacho O, Mattio L, Draisma S, Fredericq S, Diaz-Pulido G. Morphological and molecular assessment of Sargassum (Fucales, Phaeophyceae) from Caribbean Colombia, including the proposal of Sargassum giganteum sp. Nov., Sargassum schnetteri comb. nov. and Sargassum section Cladophyllum sect. Nov. Systematics and Biodiversity. 2015; 13(2): 105-30.
15. Win NN, Hanyuda T, Draisma SG, Furnari G, Meinesz A, Kawai H. Padina ditristromatica sp. Nov. and Padina pavonicoides sp. nov.(Dictyotales, Phaeophyceae), two new species from the Mediterranean Sea based on morphological and molecular markers. European Journal of Phycology. 2011; 46(4): 327-41.
16. Silberfeld T, Bittner L, Fernández‐García C, Cruaud C, Rousseau F, de Reviers B, Leliaert F, Payri CE, De Clerck O. Species diversity, phylogeny and large scale biogeographic patterns of the genus Padina (Phaeophyceae, Dictyotales). Journal of Phycology. 2013; 49(1): 130-42.
17. Hammed AM, Jaswir I, Simsek S, Alam Z, Amid A. Enzyme aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed. International Food Research Journal. 2017; 24(4): 1660.
18. Xin L, Bin L, Xiao-Lei W, Zhen-Liang S, Chang-Yun W. Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum. Czech Journal of Food Sciences. 2016 Nov 1; 34(5): 406-13.
19. Amorim RD, Rodrigues JA, Holanda ML, Quinderé AL, Paula RC, Melo VM, Benevides NM. Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed Gracilaria ornata. Brazilian Archives of Biology and Technology. 2012; 55(2): 171-81.
20. Morán-Santibañez K, Cruz-Suárez LE, Ricque-Marie D, Robledo D, Freile-Pelegrín Y, Peña-Hernández MA, Rodríguez-Padilla C, Trejo-Avila LM. Synergistic effects of sulfated polysaccharides from Mexican seaweeds against measles virus. BioMed Research International. 2016; 1: 2016.
21. Ramya S, Padma Priya N, Sheela R, Poonghuzali TV. Effect of environmental factors and biological parameters of edulis and kappaphycus alvarezii from Rameshwaram Mandapam Coast India. International Research Journal of Biological Sciences. 2019; 8(8): 1-7.
22. Jayaraman JD, Sigamani S, Venkatachalam H, Ramamurthy D. Extraction and Purification of Sulfated Polysaccharide from Brown Algae and its Efficacy in Preventing Blood Clotting. Asian Journal of Biological and Life Sciences. 2016; 5(3).
23. Roy S, Anantharaman P. Biochemical Compositions of Seaweeds Collected from Olaikuda and Vadakkadu, Rameshwaram, Southeast Coast of India. Journal Marine Science and Research Development. 2017; 7(240):2.
24. Agili FA, Mohamed SF. Polysaccharides from Padina pavonia: Chemical structural and antioxidant activity. Australian Journal of Basic and Applied Sciences. 2012; 6(5): 277-83.
25. Imjongjairak S, Ratanakhanokchai K, Laohakunjit N, Tachaapaikoon C, Pason P, Waeonukul R. Biochemical characteristics and antioxidant activity of crude and purified sulfated polysaccharides from Gracilaria fisheri. Bioscience, Biotechnology, and Biochemistry. 2016; 80(3): 524-32.
26. Badrinathan S, Shiju TM, Christa AS, Arya R, Pragasam V. Purification and structural characterization of sulfated polysaccharide from Sargassum myriocystum and its efficacy in scavenging free radicals. Indian Journal of Pharmaceutical Sciences. 2012; 74(6): 549.
27. Lima I, Martinez S, Teixeira V, Gonzalez W. Morphological Analysis by Scanning Electron Microscopy of Dictyota Menstrualis in Natura and Following an Extraction Process. Chemical Engineering Transactions. 2019; 75: 571-6.
28. Gomes DL, Melo KR, Queiroz MF, Batista LA, Santos PC, Costa MS, Almeida-Lima J, Camara RB, Costa LS, Rocha HA. In vitro studies reveal antiurolithic effect of antioxidant sulfated polysaccharides from the green seaweed Caulerpa cupressoides var flabellata. Marine Drugs. 2019; 17(6): 326.
29. Olasehinde TA, Mabinya LV, Olaniran AO, Okoh AI. Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities. International Journal of Food Properties. 2019; 22(1):100-10.
30. Shi CS, Sang YX, Sun GQ, Li TY, Gong ZS, Wang XH. Characterization and bioactivities of a novel polysaccharide obtained from Gracilariopsis lemaneiformis. Anais da Academia Brasileira de Ciências. 2017; 89(1): 175-89.
31. Berri M, Slugocki C, Olivier M, Helloin E, Jacques I, Salmon H, Demais H, Le Goff M, Collen PN. Marine-sulfated polysaccharides extract of Ulva armoricana green algae exhibits an antimicrobial activity and stimulates cytokine expression by intestinal epithelial cells. Journal of Applied Phycology. 2016; 28(5): 2999-3008.
32. Krichen F, Karoud W, Sila A, Abdelmalek BE, Ghorbel R, Ellouz-Chaabouni S, Bougatef A. Extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. International Journal of Biological Macromolecules. 2015; 75: 283-9.
33. Jun JY, Jung MJ, Jeong IH, Yamazaki K, Kawai Y, Kim BM. Antimicrobial and antibiofilm activities of sulfated polysaccharides from marine algae against dental plaque bacteria. Marine Drugs. 2018; 16(9):301.
34. Marudhupandi T, Kumar TA, Senthil SL, Devi KN. In vitro antioxidant properties of fucoidan fractions from Sargassum tenerrimum. Pakistan Journal of Biological Sciences. 2014; 17(3): 402.
35. Vijayabaskar P, Vaseela N, Thirumaran G. Potential antibacterial and antioxidant properties of a sulfated polysaccharide from the brown marine algae Sargassum swartzii. Chinese Journal of Natural Medicines. 2012; 10(6): 421-8.
36. Vishwakarma J, Parmar V, Vavilala SL. Nitrate stress-induced bioactive sulfated polysaccharides from Chlamydomonas reinhardtii. Biomedical Research Journal. 2019; 6(1):7.
37. Souza MC, Marques CT, Dore CM, da Silva FR, Rocha HA, Leite EL. Antioxidant activities of sulfated polysaccharides from brown and red seaweeds. Journal of Applied Phycology. 2007; 19(2): 153-60.
38. Palpperumal S, Harinathan B, Sankaralingam S, Shankar T, Mahendran S, Kathiresan D, Sivakumar N. Antioxidant Activities of Sulfated Polysaccharide Obtained from Green Seaweed Ulva lactuca L. in Tuticorin Coast, Gulf of Mannar, South East Coast of India. International Journal of Microbiological Research. 2019; 10 (1): 16-23.