Author(s):
Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem
Email(s):
rashaeldalawy@gmail.com , rashaeldalawy@uomustansiriyah.edu.iq
DOI:
10.52711/0974-360X.2021.00794 
Address:
Rasha Eldalawy1*, Widad M K Al-Ani2, Wasan Abdul Kareem3
1Pharmacognosy and Medicinal Plants Department, College of Pharmacy, Mustansiriyah University, Baghdad – Iraq.
2Ashur University College, Baghdad- Iraq.
3Clinical Laboratory Science Department, College of Pharmacy, Mustansiriyah University, Baghdad – Iraq.
*Corresponding Author
Published In:
Volume - 14,
Issue - 9,
Year - 2021
ABSTRACT:
Milk thistle's extract by an organic solvent is known as silymarin, which is a mixture of about 75% flavolignan and trace content of flavonoid (mainly taxifolin), other ingredients involve fatty acids and polyphenolic compounds. The major flavolignans are silybin, isosilybin, silydianin, and silychristin, quantitatively silybin is considered as the major compound followed by isosilybin, and both are represent approximately 60% of silymarin. This study was designed to determine the concentration of silybin in different parts of the plant and to study the effect of extraction methods, solvents and the time of collection on silybin concentration, which have been done by HPLC instrument. The results show that the higher yield of silybin is obtained from the seed using ethanol in the soxhlet apparatus and that all other parts of the plant which are produced in huge amount by the plant also contain a good concentration of silybin when compared with seeds, So they can be used as an alternative source for silybin production.
Cite this article:
Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem. Quantitative estimation of Silybin in Iraqi Silybum marianum by HPLC. Research Journal of Pharmacy and Technology. 2021; 14(9):4567-2. doi: 10.52711/0974-360X.2021.00794
Cite(Electronic):
Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem. Quantitative estimation of Silybin in Iraqi Silybum marianum by HPLC. Research Journal of Pharmacy and Technology. 2021; 14(9):4567-2. doi: 10.52711/0974-360X.2021.00794 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-8
REFERENCES:
1. Ahmed H. M. Ethnopharmacobotanical study on the medicinal plants used by herbalists in Sulaymaniyah province Kurdistan Iraq. Journal of Ethnobiology Ethnomedicine 2016; 12(8).
2. Huda K. A., Muthanna I. A. and Ghaith A. J. Pharmacological and pharmacognostical activity of Silybum marianum (review article). Al Mustansiriyah Journal of Pharmaceutical Science. 2020; 20(3): 72-82.
3. Marie F., Alena N., Jitka V., Petra J., Zbynek D., Tomas R., Vladimir K., Jana H., Libor V. and Milena S. Z. Poor chemical and microbiological quality of the commercial milk thistle based dietary supplements may account for their reported unsatisfactory and non reproducible clinical outcomes. Scientific Reports 2019; 9(1): 1-12.
4. Karanje A. S., Kandale J. B., Jadhav R. and Patil M. Formulation development and evaluation of silymarin gel. Research Journal of Pharmacy and Technology. 2011; 4(10): 1633-1636.
5. David JK, Heather SS, and Nicholas HO. Milk Thistle Nomenclature: Why It Matters in Cancer Research and Pharmacokinetic Studies. Integrative Cancer Therapy. 2007; 6(2): 110-119.
6. Ludovico A, Angelo AI, Natasa M, Carla C, Antonello S, and Raffaele C. Milk thistle (Silybum marianum): A concise overview on its chemistry, pharmacological, and nutraceutical uses in liver disease. Phytotherapy Research. 2018; 1-12.
7. Abel FF, Gabriel FC and Roberto KC. Hepatoprotective effect of silymarin (Silybum marianum) on hepatotoxicity induced by acetaminophen in spontaneously hypertensive rats. Evid Based Complement Alternative Medicine 2015; 2015: 538317.
8. Prashant T., Kuldeep K., Rajinikant P., Ashish P. and Pratap K. S. Hepatopotective potentials of Butea monosperma stem bark extract against carbon tetrachloride induced hepatotoxicity in albino rats. Research Journal of Pharmacology and Pharmacodynamics 2011; 3(5): 281-284.
9. Ghawate V. B., Purnima S. and Bhambar R. S. Hepatoprotective activity of Bridelia retusa bark extracts against carbon tetrachloride induced liver damage rats. Research Journal of Pharmacognosy and Phytochemistry 2017; 9(2): 121-124.
10. Jitendra P., Venkateshwar R. and Kumar G. S. Evaluation of hepatoprotective activity of ethanolic extract of Dispyros melanoxylon (Roxb) leaves against CCl4 induced hepatotoxicity in albino rats. Research Journal of Pharmacy and Technology 2015; 8(5): 571-574.
11. Saeed M, Babazadeh D, Arif M andArain MA. Silymarin: Apotent hepatoprotective agent in poultry industry. Cambridge University press 2017; 37(3): 483-492.
12. Irfan M. S., Bhambere D. S. and Dr. S. K. Formulation of silymarin loaded PLGA nanoparticle for liver targeting. Asian Journal of Pharmacy and Technology 2017; 7(4): 209-220.
13. Jia-ping W, Chin-chuan T, Yu-lan Y, Yueh-min L, Chien-chung L, Cecilia HD, Chia-yao S, Vijaya P, Lung-fa P and Chih-yang H. Silymarin accelerates liver regeneration after partial hepatectomy. Evid Based Complement Alternate Med. 2015; 2015: 603529
14. Suleyman C, Serhat T, Bulent CB, Hasan E, Recep A, Serpil C, Huseyin SE, Atilla I, Selim S and Yilmaz P. The effect of silymarin on hepatic regeneration after partial hepatectomy: is silymarin effective in hepatic regeneration? International Journal of Clinical and Experimental Medicine 2015; 8(2): 2578-2585.
15. Eldalawy R. Quantitative estimation of rutin in Rue (Ruta graveolen L.) cultivated in Iraq with the evaluation of its antioxidant activity. Asian Journal of Pharmaceutical and Clinical Research. 2017; 10 (2): 353-355.
16. Anwar M., Widad M. K.A. and Ayad R. Isolation of astragalin from Iraqi Chenopodium album. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(12): 530-535.
17. Huda K. A., Ibrahim S. A., Rasha E., Noor M. N. Antimicrobial and Antioxidant activity of Pinus halepensis Miller cone extract which grown in Iraq. Research Journal of Pharmacy and Technology. 2018; 11(11): 4977-4980.
18. Zahraa A. A., Huda K. A., Fatima M. S. and Shahad Q.I. GC-Mass and phytochemical investigation of Cymbopogon citrates. Research Journal of Pharmacy and Technology 2019; 12(1): 67-73.
19. Azwanlda N. N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants 2015; 4(3): 1000196.
20. Abbassia B, Hassiba MB and Imene S. Effects of different extraction methods and solvents on the phenolic composition and antioxidant activity of Silybum marianum leave extracts. International Journal of Medical Science and Clinical Invention 2018; 5(03): 3641-3647.
21. Suha H. A. and Enas J. K. Identification of silymarin in Echinopus tenuisectus family Compositae. Biotechnology Research Center Journal 2007. 1: 27-42.
22. David T and George K.V. HPTLC analysis of the leaf extract of Hydnocarpus pendulus Manilal, Sabu and Sivarajan. International Journal of Pharmacy and Life Sciences 2014; 5(4): 3452-3462.
23. Gupta M., Banerjee D. and Mukherjee A. Studies of anti inflammatory, antipyretic and analgesic effects of aqueous extract of traditional herbal drug on rodents. International Research Journal of Pharmacy 2013; 4(3): 113-120.
24. Omar A. A., Hadad G. M. and Badr J. M. First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in Egypt during different growth stage. Acta Chromatographica 2012; 24(3): 463-474.
25. Murali M., Satyanarayana V. V., Haribabu B. and Krishnamurthy M. A RP-HPLC method for the simultaneous estimation of silymarin and thiamine in bulk and pharmaceutical forms. Asian Journal of Research in Chemistry 2012; 5(12): 1440-1443.