Author(s): Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal

Email(s): ,

DOI: 10.52711/0974-360X.2021.00837   

Address: Veena Devi Singh1*, Vijay Kumar Singh2, Sanjay J Daharwal3
1Assistant Professor, Noida Institute of Engineering and Technology (Pharmacy Institute), Greater Noida, Uttar Pradesh, India – 201306.
2Associate Professor, School of Medical and Allied Sciences, Galgotias University, Greater Noida, Uttar Pradesh, India.
3Associate Professor, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India – 492010.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021

This research work was emphasis to adopt green analytical chemistry via development of environmentally friendly methods for simultaneously estimation of drugs used for chemotherapy induced nausea and vomiting (CINV). In these study two multivariate calibration methods namely; Partial least square (PLS) models, Principal component regression (PCR) and RP-HPLC (Reverse phase- high performance liquid chromatography) method were employed for simultaneous assessment of aprepitant (APT), dexamethsone (DEX) and ondansetron (OND) in their market formuations. The chromatographic separation was achieved on Phenomenex Luna C18 column (150mm X 4.6mm in diameter with 5µ particle size) and detection was carried out by UV-Visible detector. The mobile phase comprises a mixture of ethanol and toluene in a ratio of 65:35% v/v, at the flow rate of 1.2ml/ min. The elution was monitored at 225nm and total run time required for separation was 10 min. The retention time of APT, DEX and OND were found to be 4.37 min, 6.57 and 8.11 min respectively. The Applied methods were validated as per ICH guidelines to achieve maximum sensitivity and lowest error. Linearity range for multivariate calibration methods was found to be 5-50µg/mL for APT, DEX and OND and similarly for HPLC methods was found to be 5-30µg/mL,5-30µg/mL and 5-50µg/mL of APT, DEX and OND respectively. A statistical procedure was carried out to find statistical difference among these developed methods. The results revealed that there is no significant difference between two multivariate models and HPLC methods. Therefore, it could be applied as an alternative of HPLC method in quality control laboratories lacking the required facilities for these expensive instruments. Hence, proposed method can also be applied in clinical pharmacy, toxicology and quality control analysis without the interference of commonly encountered dosage form additives.

Cite this article:
Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal. The comparison of two Chemometric Assisted UV Spectrophotometric Techniques with High-performance Liquid Chromatography Methods for simultaneous determination of three Antiemetic drugs used in Chemotherapy Induced Nausea and Vomiting. Research Journal of Pharmacy and Technology. 2021; 14(9):4815-4. doi: 10.52711/0974-360X.2021.00837

Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal. The comparison of two Chemometric Assisted UV Spectrophotometric Techniques with High-performance Liquid Chromatography Methods for simultaneous determination of three Antiemetic drugs used in Chemotherapy Induced Nausea and Vomiting. Research Journal of Pharmacy and Technology. 2021; 14(9):4815-4. doi: 10.52711/0974-360X.2021.00837   Available on:

1.    M.A. Tantawy, S. Alweshahy, D.A. Elshabasy, N. F. Youssef, Simultaneous Determination of co-administrated deflazacort, aprepitant and granisetron in dosage forms and spiked human plasma by RP-HPLC/PA, J. Chromatogr. Sci., 57(9) (2019) 790–798,
2.    T. Schmitt, H. Goldschmidt, K. Neben, A. Freiberger, J. Husing, M. Gronkowski, M. Thalheimer, L.H. Pelzl, G. Mikus, J. Burhenne, A. D Ho, G. Egerer, Aprepitant, granisetron, and dexamethasone for prevention of chemotherapy-induced nausea and vomiting after high-dose melphalan in autologous transplantation for multiple myeloma: Results of a randomized, placebo-controlled phase III trial; J. Clin. Oncol., 32(30) (2014) 3413-20.
3.    R. Schoffelen, A. G. Lankheet, C. M. L. van Herpen, J. J. M. van der Hoeven, I. M. E. Desar, C. Kramers, Drug-drug interactions with aprepitant in antiemetic prophylaxis for chemotherapy, Neth. J. Med., 76 (3) (2018)109-114.
4.    Martindale; The complete drug reference, 39th ed. Pharmaceutical Press, London, (2018).
5.    www. DB08930
6.    T. Benjamin, C. H. Rajyalakshmi, C. Rambabu, Derivative spectrophotometric methods for determination of aprepitant in bulk and pharmaceutical formulation. Der Pharma Chemica. 5(1) (2013) 156-160.
7.    M.L. Constanzer, C.M. Chavez-Eng, J. Dru, W.F. Kline, B.K. Matuszewki, Determination of a novel substance P inhibitor in human plasma by high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometric detection using single and triple quadrupole detectors. J Chromatogr B Anal Tech Biomed and Life Sci. 807 (2004) 243–250.
8.    USP; The United States Pharmacopeia 41 National Formulary 36, United states pharmacopeial convention, Rockville, MD, USA, (2018).
9.    D. Wu, D.J. Paul, X. Zhao, S.D. Douglas, J.S. Barrett, A sensitive and rapid liquid chromatography-tandem mass spectrometry method for the quantification of the novel neurokinin-1 receptor antagonist aprepitant in rhesus macaque plasma, and cerebral spinal fluid, and human plasma with application in translational Neuro-AIDs research. J Phar. Biomed Anal, 49 (2009) 739–745. Epub 2008 Dec 13.
10.    E. Rosenblatt, L. M. Lev, and E. Robinson, High-dose dexamethasone and high-dose metoclopramide versus high-dose dexamethasone and sulpiride in the management of cisplatin-induced emesis, Oncol., 45(4) (1988) 297–299,
11.    www. DB00963
12.    G.B. Pena, D.L. Gonzalo, R.H. Izquierdo, R.M. Santos A Method development for betamethasone and dexamethasone by micellar liquid chromatography using cetyl trimethyl ammonium bromide and validation in tablets. Application to cocktails, J. Pharm. Biomed. Anal. 36 (2004) 65-70.
13.    R. B. Friedrich, A. Ravanello, L.C. Cichota, C. M. B. Rolim, R. C. R. Beck, Validation of a simple and rapid UV spectrophotometric method for dexamethasone assay in tablets, Quím. Nova 32(4) (2009) 1052-1054,
14.    D. Chothani, J. Bhalani, K. R. Vadaliya, Ratio derivative uv spectroscopy method for simultaneous estimation of moxifloxacin hydrochloride and dexamethasone sodium phosphate in pharmaceutical dosage form, Inventi Rapid: Pharm Analysis & Quality Assurance 2013 (2013), Article Id: Inventi: Ppaqa/766/13
15.    J Bhalani, K. Vadalia, Z.R. Dedania, Validated first and second order derivative UV spectrophotometric methods for simultaneous estimation of moxifloxacin hydrochloride and dexamethasone sodium phosphate in ophthalmic dosage form, Inventi Rapid: Pharm Analysis & Quality Assurance, 2012 (2012), Article Id-Inventi: Ppaqa/424/12.
16.    M. Spangler, E. Mularz, A validated stability-indicating method for the assay of dexamethasone in drug substance and drug product analyses, and the assay of preservatives in drug product. Chromatographia, 54 (2001) 329,
17.    M.J. Dabhi, A. H. Patwari, U. H. Desai, D. B. Doshi, I. S. Rathod and B. N. Suhagia, Simultaneous determination of moxifloxacin hydrochloride and dexamethasone sodium phosphate in eye drops by HPLC and absorbance correction method. J. Chem Pharm Res.4(10) (2012) 4462-4467
18.    P. Katakam, K. R. Sireesha: Simultaneous determination of ciprofloxacin hydrochloride and dexamethasone sodium phosphate in eye drops by HPLC. J. Chem. 9(3) (2012) 1077-1084
19.    J.M. Lemus Gallego, J. Perez Arroyo, Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography. J. Pharma. Biomed. Anal., 30(4) (2002) 1255-1261. doi: 10.1016/s0731-7085(02)00468-5.
20.    H. W. Kwak, J. D. Donald Amico, Determination of dexamethasone sodium phosphate in vitreous by high performance liquid chromatography. Korean J. ophthalmol. 9 (1995) 79-83.
21.    H.W. Darwish, F.H. Metwally and A.E. Bayoumi, Development of three methods for simultaneous quantitative determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in oral liquids, Trop. J. Pharm. Res., 14(1) (2015) 153-161 doi:
22.    H.C. Goicoechea, M.S. Collado, M.L. Satuf, A.C. Olivieri. Complementary use of partial least-squares and artificial neural networks for the non-linear spectrophotometric analysis of pharmaceutical samples. Anal. Bioanal. Chem. 374(3) (2002) 460-465.
23.    United State Pharmacopoeia (2006), 32nd edition. United States Pharmacopieal Convention. Inc. Rockville, Md,: 3200, pp.3139.
24.    British Pharmacopoeia (2011), Published by The Stationery Office on behalf of the Medicines and Healthcare products Regulatory Agency, 1643, pp.1598.
25.    www. DB00904
26.    M. Basilio, Second-derivative spectrophotometric assay of mixtures of ondansetron sodium and ampicillin sodium in pharmaceutical, J. Pharm. Sci, 77 (1988) 1042-1046.
27.    R. Sheshala, Y. Darwis, N. Khan, Development and validation of an RP–LC–UV method for the determination of ondansetron. application to pharmaceutical dosage forms. Chromatographia, 70 (2009) 75-81,
28.    R.F. Moreira, M.C. Salvadori, C.P. Azevedo, S.D. Oliverra, D.C. Borges, R.A. Moreno Development and validation of a rapid and sensitive LC-ESI-MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study. Biomed Chromatogr., 24(11) (2010) 1220–1227,
29.    F. Bourdon, M. Lecoeur, P. Odou, C. Vaccher, C. Foulon, Complementarity of UV-PLS and HPLC for the simultaneous evaluation of antiemetic drugs, Talanta, 120 (2014) 274–282,
30.    F.C. Chen, L.H. Wang, J. Guo, X.Y. Shi, B.X. Fang, Simultaneous determination of dexamethasone, ondansetron, granisetron, tropisetron, and azasetron in infusion samples by HPLC with DAD detection; J. Anal. Methods Chem., 2017 (2017) 6749087,
31.    A. Hemdan, M.S. Eissa, Simultaneous chromatographic analysis of sofosbuvir/ledipasvir in their combined dosage form: an application to green analytical chemistry, J. Anal. Sci. Technol. 10(39) (2019) 1-12.
32.    V.D. Singh, S.J. Daharwal, Development and validation of multivariate calibration methods for simultaneous estimation of Paracetamol, Enalapril maleate and hydrochlorothiazide in pharmaceutical dosage form, Spectrochim. Acta A Mol. Biomol. Spectrosc. 171 (2017) 369–375, https://
33.    T. Eticha, G. Kahsay, F. Asefa, T. Hailu, H. Gebretsadik, T. Gebretsadikan, B. Thangabalan, Chemometric-Assisted spectrophotometric method for the simultaneous determination of ciprofloxacin and doxycycline hyclate in pharmaceutical formulations, J. Anal. Methods Chem. 2018 (2018) 9538435,
34.    D.M. Haaland, E.V. Thomas, Partial least-squares methods for spectral analyses. 1. Relation to other quantitative calibration methods and the extraction of qualitative information, Anal. Chem. 60 (1988) 1193–1202,
35.    International Conference on Harmonization, (ICH) Q2B, Validation of Analytical Procedures and Methodology, US FDA Federal Register, 1997, pp. 62.
36.    V.D. Singh, V.K. Singh, Chemo-metric assisted UV-spectrophotometric methods for simultaneous estimation of darunavir ethanolate and cobicistat in binary mixture and their tablet formulation, Spectrochim. Acta A Mol. Biomol. Spectrosc. 250(5) (2021) 119383,

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