Author(s): Selvaraj Divya, Subramanian Arivoli, Samuel Tennyson

Email(s): samtennyson@gmail.com

DOI: 10.52711/0974-360X.2021.00821   

Address: Selvaraj Divya1, Subramanian Arivoli1, Samuel Tennyson2*
1Department of Zoology, Thiruvalluvar University, Vellore 632115, Tamil Nadu, India.
2Department of Zoology, Madras Christian College, Chennai 600059, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021


ABSTRACT:
Medicinal plants are of great significance and global importance as antioxidants and antibacterial agents. In the present investigation the chloroform leaf extract of Abelmoschus manihot was screened for its phytochemical constituents, antioxidant and antibacterial properties. Flavonoids, phenolics, tannins and vitamins were the phytochemical constituents present. GC-MS analysis revealed ten major phytocompounds. In vitro antioxidant assay via DPPH exhibited an IC50 value of 234.7±37.53mg/mL, and its activity could be ascribed due to the presence of the phytocompound a-tocopherol. The antimicrobial activity represented by the zone of inhibition via disc diffusion method, observed no activity against the fungal strains, Aspergillus niger and Candida albicans. Staphylococcus aureus and Escherichia coli exhibited 12 to 14mm zone of inhibition at concentrations 100-500µg, which can be attributed due to the presence of flavonoids. The above results suggests that antioxidant and antibacterial activity exhibited by the chloroform leaf extract of this plant was attributed to the presence of the phytochemicals, especially phenolics and flavonoids. Further in depth studies on the phytochemical constituents of this chloroform leaf extract is recommended, as this extract could serve as an additional implement to natural antioxidant and antibacterial agents.


Cite this article:
Selvaraj Divya, Subramanian Arivoli, Samuel Tennyson. Phytochemical analysis and in vitro Antioxidant and Antibacterial activity of the Chloroform leaf extract of Abelmoschus manihot (L.) Medik (Malvaceae). Research Journal of Pharmacy and Technology. 2021; 14(9):4719-6. doi: 10.52711/0974-360X.2021.00821

Cite(Electronic):
Selvaraj Divya, Subramanian Arivoli, Samuel Tennyson. Phytochemical analysis and in vitro Antioxidant and Antibacterial activity of the Chloroform leaf extract of Abelmoschus manihot (L.) Medik (Malvaceae). Research Journal of Pharmacy and Technology. 2021; 14(9):4719-6. doi: 10.52711/0974-360X.2021.00821   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-35


REFERENCES:
1.    Uddin SN, Akond MA, Mubassara S, Yesmin MN. Antioxidant and antibacterial activities of Trema cannabina. Middle-East Journal of Scientific Research. 2008; 3: 105-108.
2.    Jayasri MA, Mathew L, Radha A. A report on the antioxidant activities of leaves and rhizomes of Costus pictus D. Don. International Journal of Integrative Biology. 2009; 5(1): 20-26.
3.    Bamoniri A, Ebrahimabadi AH, Mazoochi A, Behpour M, Kashi FJ, Batooli H. Antioxidant and antimicrobial activity evaluation and essential oil analysis of Semenovia tragioides Boiss. from Iran. Food Chemistry. 2010; 122: 553‑558.
4.    Trigui M, Hsouna AB, Tounsi S, Jaoua S. Chemical composition and evaluation of antioxidant and antimicrobial activities of Tunisian Thymelaea hirsuta with special reference to its mode of action. Industrial Crops and Products. 2013; 41: 150‑157.
5.    Ebrahimabadi AH, Mazoochi A, Kashi FJ, Djafari‑Bidgoli Z, Batooli H. Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran. Food and Chemical Toxicology. 2010; 48: 1371‑1376.
6.    Tippani R, Porika M, Allenki V, Anreddy RN, Yellu NR, Krishna DR, Thammidala C, Abbagani S. Antioxidant and analgesic activities of Pterocarpus marsupium Roxb. Journal of Herbs, Spices and Medicinal Plants. 2010; 16: 63 68.
7.    Panchawat S, Rathore KS, Sisodia SS. A review on herbal antioxidants. International Journal of Pharm Tech Research. 2010; 2(1): 232-239.
8.    Zengin G, Cakmak YS, Guler GO, Aktumsek A. Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. hayekiana Wagenitz. Records of Natural Products. 2011; 5: 123-132.
9.    Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine. 2012; 12: 221.
10.    Voravuthikunchai SP, Kitpipit L. Activities of crude extracts of Thai medicinal plants on methicillin-resistant Staphylococcus aureus. Clinical Microbiology and Infection. 2003; 9: 236.
11.    Yoh M, Frimpong EK, Honda T. Effect of antimicrobial agents, especially fosfomycin on the production and release of verocytotoxin by enetrohaemorrhagic Escherichia coli 0157: H7. FEMS Immunology and Medical Microbiology. 1997; 19: 57-64.
12.    Yoh M, Frimpong EK, Voravuthikunchai SP, Honda T. Effect of subinhibitory concentrations of antimicrobial agents (quinolones and macrolides) on the production and release of verocytotoxin by enetrohaemorrhagic Escherichia coli 0157: H7. Canadian Journal of Microbiology. 1999; 45: 732-739.
13.    Anonymous. The wealth of India. A dictionary of Indian raw material and industrial products. Vol V, CSIR, New Delhi. 2005; pp. 90-91.
14.    Kirtikar KR, Basu BD. Indian medicinal plants. Vol I. 2nd ed., International Book Distributor, Dehradun. 2005; pp. 339.
15.    Todarwal A, Pritam J, Sanjay B. Abelmoschus manihot Linn: ethnobotany, phytochemistry and pharmacology. Asian Journal of Traditional Medicines. 2011; 6(1): 1-7.
16.    Onakpa MM. Ethnomedicinal, phytochemical and pharmacological profile of genus Abelmoschus. Phytopharmacology. 2013; 4(3): 648-663.
17.    Vogel AL. Text book of practical-organic chemistry. The English Language Book Society and Longman, London. 1978; pp. 1363.
18.    Harborne JB. Phytochemical methods, a guide to modern techniques of plant analysis, 3rd edition. Chapman and Hill Ltd., London. 1998; pp. 279.
19.    Van Burden TP, Robinson WC. Formation of complexes between protein and tannic acid. Journal of Agricultural and Food Chemistry. 1981; 1: 77.
20.    Obadoni BO, Ochuko PO. Studies and comparative efficacy of the crude extracts of some homostatic plants in Edo and Delta states of Nigeria. Global Journal of Pure and Applied Science 2001; 8b: 203-208.
21.    Boham A, Kocipai AC. Flavonoids and condensed tannins from leaves of Hawaiia vaccinum vaticulatum and V. calyscinium. Pacific Science. 1974; 48: 458-463.
22.    Okwu DE, Okwu ME. Phytochemicals and vitamins content of indigenous plant species of south eastern Nigeria. Journal of Sustainable Agriculture and Environment. 2004; 6: 30-37.
23.    Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agriculture and Food Chemistry. 1992; 40: 945-948.
24.    Irobi ON, Moo-Young M, Anderson WA, Daramola SO. Antimicrobial activity of the bark of Bridelia ferruginea (Euphorbiaceae). International Journal of Pharmacognosy. 1994; 34: 87-90.
25.    Sies H. Oxidative stress: oxidants and antioxidants. Experimental Physiology. 1997; 82: 291‑295.
26.    Leaves L. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides. American Journal of Ethnomedicine. 2014; 1: 244-249.
27.    Li J, Zhang J, Wang M. Extraction of favonoids from the flowers of Abelmoschus manihot (L.) Medic by modified supercritical CO2 extraction and determination of antioxidant and anti-adipogenic activity. Molecules. 2016; 21: 810.
28.    Taroreh MIR, Widiyantoro A, Murdiati A, Hastuti P, Raharjo S. Identification of flavonoid from leaves of gedi (Abelmoschus manihot L.) and its antioxidant activity. AIP Conference Proceedings. 2016; 1755: 080010.
29.    Waris R, Pratiwi AMED, Najib A. Radical scavenging activity of leaf extract of edible hibiscus (Abelmoschus manihot (L.) Medik) using 1,1- Diphenyl-2-Picryl Hydrazil (DPPH). International Journal of PharmTech Research. 2016; 9(6): 343-347.
30.    Sudewi S, Lolo WA, Warongan M, Rifai Y, Rante H. The ability of Abelmoschus manihot L. leaf extract in scavenging of free radical DPPH and total flavonoid determination. Materials Science and Engineering. 2017; 259: 1-5.
31.    Gunawan IWG, Yasa IWPS, Putra AAB. Response oxidative stress of ethanol extracts Abelmoschus manihot L leaf on the activity of superoxide dismutase enzyme in diabetic Wistar rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2018; 9(4): 298-305.
32.    Banne Y, Ponidjan TS, Dumanauw JM. Antioxidant and hepatoprotective activity of Abelmoschus manihot L. Medik leaf fraction against CCl4 –induced liver damage in rats. International Journal of Applied Pharmaceutics. 2019; 11(3): 17-19.
33.    Chumbhale DS, Chavan MJ, Upasani CD. Evaluation of Abelmoschus manihot (L.) Medik flowers for free radical scavenging activities using in vitro assays. International Journal of Biology, Pharmacy and Allied Sciences. 2020; 9(2): 70-79.
34.    Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal. 2013; 21: 143-152.
35.    Esmaeili MA, Sonboli A. Antioxidant, free radical scavenging activities of Salvia brachyantha and its protective effect against oxidative cardiac cell injury. Food and Chemical Toxicology. 2010; 48: 846‑853.
36.    Kilani‑Jaziri S, Bhouri W, Skandrani I, Limem I, Chekir‑Ghedira L, Ghedira K. Phytochemical, antimicrobial, antioxidant and antigenotoxic potentials of Cyperus rotundus extracts. South African Journal of Botany. 2011; 77: 767‑776.
37.    Bharti R, Ahuja G, Sujan GP, Dakappa SS. A review on medicinal plants having antioxidant potential. Journal of Pharmacy Research. 2012; 5(8): 4278-4287.
38.    Ebrahimabadi AH, Ebrahimabadi EH, Djafari‑Bidgoli Z, Kashi FJ, Mazoochi A, Batooli H. Composition and antioxidant and antimicrobial activity of the essential oil and extracts of Stachys inflata Benth from Iran. Food Chemistry. 2010; 119: 452‑458.
39.    Castilho PC, Savluchinske‑Feio S, Weinhold TS, Gouveia SC. Evaluation of the antimicrobial and antioxidant activities of essential oils, extracts and their main components from oregano from Madeira island, Portugal. Food Control. 2012; 23: 552‑528.
40.    Tiwari P, Kumar B, Kaur M, Kaur G. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia. 2011; 1(1): 98-106.
41.    Laidman DL, Gaunt JK, Hall GS, Broad CT. Extraction of tocopherols from plant tissues. Methods in Enzymology. 1971; 18(Part C): 366-369.
42.    Hannan PA, Khan JA, Ullah I, Ullah S. Synergistic combinatorial antihyperlipidemic study of selected natural antioxidants; modulatory effects on lipid profile and endogenous antioxidants. Lipids in Health and Disease. 2016; 15(1): 151.
43.    Onibi GE, Scaife JR, Murray I, Fowler VR. Supplementary α-tocopherol acetate in full-fat rapeseed-based diets for pigs: effect on performance, plasma enzymes and meat drip loss. Journal of Agricultural and Food Chemistry. 80(11): 1617-1624.
44.    Munn´e-Bosch S. The role of α-tocopherol in plant stress tolerance. Journal of Plant Physiology. 2005; 162(7): 743-748.
45.    Hollman PCH. Evidence for health effects of plant phenols: local or systemic effects? Journal of the Science of Food and Agriculture. 2001; 81: 842-852.
46.    Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry. 2005; 53(10): 4290-4302.
47.    Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health: A review. Critical Reviews in Food Science and Nutrition. 1998; 38: 421-464.
48.    Sahari MA, Ardestani SB. Bio-antioxidants activity: Their mechanisms and measurement methods. Applied Food Biotechnology. 2014; 2(1): 3-8.
49.    Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. African Journal of Traditional, Complementary and Alternative Medicines. 2008; 5: 61-73.
50.    Evans RCA, Miller NJ, Bowell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research. 1995; 22(4): 375-383.
51.    Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osong Public Health and Research Perspectives. 2013; 4(5): 246-254.
52.    Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry. 2000; 48(10): 4581-4589.
53.    Oszmianski J, Wojdylo A, Lamer-Zarawska E, Swiader K. Antioxidant tannins from Rosaceae plant roots. Food Chemistry. 2007; 100(2): 579-583.
54.    Uttara J, Mohini U. Evaluation of antioxidant activity of aqueous extract bark of Ficus glomerata. Research Journal of Pharmacy and Technology. 2008; 1(4): 537-538.
55.    Soni H, Nayak G, Mishra K, Singhai AK, Pathak AK. Evaluation of phyto pharmaceutical and atioxidant potential of methanolic extract of peel of Punica granatum. Research Journal of Pharmacy and Technology. 2010; 3(4): 1170-1174.
56.    Gunalan G, Kumar SM, Sangeetha N. Preliminary phytochemical analysis and in vitro oxidant scavenging activity of Rosemary officinalis. Research Journal of Pharmacy and Technology. 2011; 4(10): 1588-1590.
57.    Mahadik SU, Jagtap VN, Oswal H, Kumawat N, Kothari R. In vitro antioxidant activity of ethanolic extracts of Celosia argentea aerial parts, fresh fruits of Fragaria vesca, Tamarindus indica, Psidium guajava, Zizyphus mauritiana. Research Journal of Pharmacy and Technology. 2011; 4(11): 1782-1784.
58.    Pal R, Girhepunje K, Shrivastava N, Hussain MM, Thirumoorthy N. Antioxidant and free radical scavenging activity of ethanolic extract of Morinda citrifolia. Research Journal of Pharmacy and Technology. 2011; 4(8): 1224-1226.
59.    Kanagavalli M., Anuradha R. A study on phytochemical constituents and in vitro antioxidant activity of Carica papaya. Research Journal of Pharmacy and Technology. 2012; 5(1): 119-120.
60.    Yao YM. Inhibition effect of the flower of Abelmoschus manihot extraction on Diplococcus gonorrhoeae. Jiangsu Journal of Traditional Chinese Medicine. 1994; 15: 43-49.
61.    Pritam J, Sanjay B. Antimicrobial properties of Abelmoschus manihot Linn. International Journal of Pharm Tech Research. 2011; 3: 29-32.
62.    Zamrul LY, Hartati, Parawansah. Antibacterial activity test on ethanol extract of gedi leaf (Abelmoschus manihot L.) on the growth of Escherichia coli and Staphylococcus aureus. Medula. 2019; 6: 583-590.
63.    Yahaya A, Idris A. Antibacterial activity and phytochemical screening of Carica papaya on some enteric bacterial isolates of public health importance. Greener Journal of Biological Sciences. 2017; 7(1): 1-7.
64.    Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Scientific World Journal. 2013; 1(1): 1-16.
65.    Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from natural sources. Journal of Antimicrobial Chemotherapy. 2007; 59(6): 1247-1260.
66.    Moyo B, Masika PJ, Muchenje V. Antimicrobial activities of Moringa oleifera Lam extracts. African Journal of Biotechnology. 2012; 11(11): 2797-2802.
67.    Jones G, McAllister T, Muir A, Cheng KJ. Effects of sainfoin (Onobrychis viciifolia Scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Applied and Environmental Microbiology.1994; 60(4): 1374-1378.
68.    Kumar AS, Mazumder A, Vanitha J, Ganesh M, Venkateshwaran K, Saravanan VS, Sivakumar T. Antibacterial activity of methanolic extract of Sesbania grandiflora (Fabaceae). Research Journal of Pharmacy and Technology. 2008; 1(1): 59-60.
69.    Patra A, Jha S, Murthy PN, Vaibhav AD. Anthelmintic and antibacterial activities of Hygrophila spinosa T. Anders. Research Journal of Pharmacy and Technology. 2008; 1(4): 531-532.
70.    Samanta A, Jena AK, Sinha C, Ghosh S, Mandal A. Antibacterial and pharmacological evaluation of root extract of plant Achyranthes aspera (Amaranthaceae). Research Journal of Pharmacy and Technology. 2010; 3(3): 910-914.
71.    Das SK, Dhake AS, Nayak A, Das NB, Pandeya SN. Antibacterial and antifungal activity of aerial part of plant Ammannia baccifera Linn. Research Journal of Pharmacy and Technology. 2011; 4(3): 430-432.
72.    Manore D, Pillai S, Joshi A, Punashiya R. Preliminary phytochemical screening and antibacterial activity of ethyl acetate extract of Cuscuta reflexa Roxb. Research Journal of Pharmacy and Technology. 2012; 5(1): 79-82.
73.    Thota P, Bhogavalli PK, Chenreddy SK. Phytochemical investigation and antibacterial screening of leaf extracts of Sapindus saponaria Vahl. Research Journal of Pharmacy and Technology. 2012; 5(2): 273-276.
74.    Simoes M, Bennett RN, Rosa EA. Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Natural Product Reports. 2009; 26(6): 746-757.
75.    Kotzekidou P, Giannakidis P, Boulamatsis A. Antimicrobial activity of some plant extracts and essential oils against food-borne pathogens in vitro and on the fate of inoculated pathogens in chocolate. LWT-Food Science and Technology. 2008; 41(1): 119-127.
76.    Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999; 12(4): 564-582.
77.    Samy PR, Gopalakrishnakone P. Therapeutic potential of plants as antimicrobials for drug discovery. Evidence-Based Complementary and Alternative Medicine. 2010; 7(3): 283-294.
78.    Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, Jabbar A. Antimicrobial natural products: An update on future antibiotic drug candidates. Natural Product Reports. 2010; 27(2): 238-254.
79.    Savoia D. Plant-derived antimicrobial compounds: Alternatives to antibiotics. Future Microbiology. 2012; 7(8): 979-990.
80.    Borges A, Ferreira C, Saavedra MJ, Simoes M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microbial Drug Resistance. 2013; 19(4): 256-265.

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