Anandhu K S, Manu Jose, Sweety Kuriakose, Jayalakshmi P M
Anandhu K S, Manu Jose*, Sweety Kuriakose, Jayalakshmi P M
Nirmala College of Pharmacy, Muvatupuzha, Ernakulam, Kerala.
Volume - 14,
Issue - 9,
Year - 2021
Diabetes Mellitus is a metabolic disorder characterized by hypoglycemia, resulting from absolute or relative deficiency of insulin. Worldwide about 220million people affected. Low-cost herbal treatment is recommended due to their lesser side effect. The aim of the current study was to determine the antidiabetic activity of aqueous leaf extracts of Lagerstroemia speciosa (Lythraceae) and Aegle marmelos (Rutaceae) using alpha amylase inhibition assay and glucose diffusion inhibition assay. In alpha amylase inhibition assay, the leaf extracts of A.marmelos (at a concentration 100µg/ml) exhibited 54.68% of a amylase inhibitory activity with an IC 50 values 92.04µg/ml whereas the leaf extracts of L. speciosa (at a concentration of 100µg/ml) exhibited 60.36% of a amylase inhibitory activity with an IC50 value of 68.19µg/ml .However the mixture of plant extracts (at a concentration of 100µg/ml) exhibited 94.87% inhibition with an IC50 value of 13.145µg/ml. Both plant extracts showed appreciable a amylase inhibitory effects, but the mixture showed more inhibitory effects than the individual plant extracts. In glucose diffusion inhibition assay, the aqueous extract of A. marmelos leaves exhibited maximum glucose diffusion inhibition (76.886%) at 150 minutes as well as L. Speciosa leaf extract showed the maximum inhibition of 79.5357% at the same time interval. The mixture of plant extracts exhibited 87.4274% inhibition at 150 minutes which produces more effects than the two plants.
Cite this article:
Anandhu K S, Manu Jose, Sweety Kuriakose, Jayalakshmi P M. Phytochemical analysis and In vitro Antidiabetic activity of aqueous extract of Lagerstroemia speciosa and Aegle marmelos. Research Journal of Pharmacy and Technology. 2021; 14(9):4697-1. doi: 10.52711/0974-360X.2021.00816
Anandhu K S, Manu Jose, Sweety Kuriakose, Jayalakshmi P M. Phytochemical analysis and In vitro Antidiabetic activity of aqueous extract of Lagerstroemia speciosa and Aegle marmelos. Research Journal of Pharmacy and Technology. 2021; 14(9):4697-1. doi: 10.52711/0974-360X.2021.00816 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-30
1. Anees A Siddiqui, Shadab A Siddiqui, Suhail Ahmad. Diabetes: mechanism, pathophysiology and management. A review. International Journal of Drug Development and Research. 2011; 5(2); 1-23.
2. American Diabetes Association. Standards of medical care in diabetes. Diabetes Care. 2004; 27(suppl 1): s15-s35.
3. Frank B Hu. Globalization of diabetes. The role of diet, lifestyles and genes. Diabetes Care. 2011; 34(6): 1249-1257.
4. Narayan P. Yadav, C. S. Chanotia. Phytochemical and Pharmacological Profile of Leaves of Aegle Marmelos Linn. The Pharma Review. Nov - Dec.2009; 144-150.
5. Marypatience Muyuka. Invitro Studies on Antidiabetic Effect of Aegle Marmelos Plant Aqueous Extract. International Journal of Science and Research (IJSR). 2016; 5(5): 252-255.
6. Karthika U, Kaval Reddy Prasasvi, T. Diana Victoria, Didi Chinnu Raju. Antioxidant potential of aqueous extract of Aegle marmelos leaves. Research Journal of Pharmacy and Technology. 2016; 9(4): 391-393.
7. Reddy VP, Urooj A. Antioxidant properties and stability of Aegle marmelos leaves extracts. J Food Sci Technol 2013; 50(1): 135–140.
8. Nirmala Gupta, R C Agarwal, Vincy Shrivastava, Amit Roy, Pushpa Prasad. Invitro antioxidant activity and Phytochemical screening of Aegle marmelos extracts. Research Journal of Pharmacognosy and Phytochemistry. 2012; 2(4): 80-83.
9. Upadhya S, Shanbhag KK, Suneetha G, Balachandra Naidu M, Upadhya S. A study of hypoglycemic and antioxidant activity of Aegle marmelos in alloxan induced diabetic rats. Indian J Physiol Pharmacol. 2004; 48(4): 476–480.
10. Prince PS, Rajadurai M. Preventive effect of Aegle marmelos leaf extract on isoprenaline induced myocardial infarction in rats: biochemical evidence. J Pharm Pharmacol .2005; 57(10): 1353–1357.
11. Rahul Vikram Singh, Prabhakar Semwal, Taranjeet Kapoor. Medicinal Potential of six different plant species of Dehradun District, Uttarakhand. Asian Journal of Research in Pharmaceutical Sciences. 2014; 4(3): 134-139.
12. Benni JM, Jayanthi MK, Suresha RN. Evaluation of the anti-inflammatory activity of Aegle marmelos (Bilwa) root. Indian J Pharmacol. 2011;43(4): 393–397.
13. Sivakumar G, Gopalasatheeskumar K, Gowtham K, Sindhu E, Akash Raj K, Rajaguru B et al. Phytochemical Analysis, Antioxidant and Antiarthritic activities of different solvent extract of Aegle marmelos L. Unripe fruit. Research Journal of Pharmacy and Technology. 2020;13(6): 2759-2763
14. Manjula A U, Prema Sampath Kumar. Invitro evaluation of Biological activity of Aegle marmelos (L) fruit. Research Journal of Pharmacy and Technology. 2016;9(4): 407-414
15. Raja SB, Murali MR, Devaraj SN. Differential expression of ompC and ompF in multidrugresistant Shigella dysenteriae and Shigella flexneri by aqueous extract of Aegle marmelos, altering its susceptibility toward β-lactam antibiotics. Diagn Microbiol Infect Dis. 2008; 61(3): 321–328.
16. G Alagamuthu, R Kirubha. Biogenic Synthesis of silver nanoparticles using Aegle marmelos fruit extract and their antibacterial potential. Asian Journal of Research in Chemistry. 2013; 6(9): 839-844.
17. Lampronti I, Martello D, Bianchi N, Borgatti M, Lambertini E, Piva R, et al. In vitro antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinal plant Aegle marmelos Correa. Phytomedicine. 2003; 10(4): 300–308.
18. Das SK, Roy C. The protective role of Aegle marmelos on aspirin-induced gastro-duodenal ulceration in albino rat model: a possible involvement of antioxidants. Saudi J Gastroenterol. 2012; 18(3): 188–194.
19. Chandaka Madhu, K Hindu, C D Sudeepthi, P Maneela, K Venkata Reddy, B Bhagyasree. Antiulcer activity of aqueous extract of Aegle maemelos leaves on Rats. Asian Journal of Pharmaceutical Research. 2012; 2(4): 132-135.
20. Kaniz Fatima Urmi, Ashrafuddin Chowdhury, Farhana Diba, Khandaker Ashfaqur Rahman, Razibul Habib, Kaiser Hamid. Evaluation of Antioxidant and cytotoxic potential of different extracts from the leaves of Aegle marmlos L. Research Journal of Pharmacy and Technology. 2013; 6(4): 384-387.
21. Eric Wei Chiang Chan, Lea Ngar Tan, Siu Kuin Wong. Phytochemistry and Pharmacology of Lagerstroemia speciosa: A Natural Remedy for Diabetes. International Journal of Herbal Medicine. 2014; 2 (2): 100-105.
22. Mohit Kotnala, G.S Chakraborthy, Avijit Mazumder. Lagerstroemia Species: A Current Review. International Journal of PharmTech Research. 2013; 5(3): 906-909.
23. Custer CD, Ranelle RA, Josephine LDY, Amelia SA and Elmer-Rico EM. Hypoglycemic activity of irradiated Banaba (Lagerstroemia speciosa) Leaves. Journal of Applied Science Research. 2005; 1(1): 95-98.
24. Suzuki Y, Unno T, Ushitani M, Hayashi K and Kakuda T. Antiobesity activity of extracts from Lagerstroemia speciosa leaves on female KK-AY mice. Journal of Nutritional Science and Vitaminology. 1999; 45: 791-795.
25. Suri S, Rawat AKS, Srivastava SK, Tuli R, Malhotra S and Gupta SK. Ellagic acid and gallic acid from Lagerstroemia speciosa inhibit HIV-1 infection through inhibition of HIV-1 protease and reverse transcriptase activity. Indian Journal Medical Research. 2013; 137(3): 540-548.
26. Fatema N, Ahmad S, Kamrunnahar. Evaluation of anti-microbial, antioxidant and cytotoxic activities of Methanolic extracts of Lagerstroemia speciosa leaves and barks. Journal of Applied Pharmaceutical Sciences. 2012; 2(10): 142-147.
27. Laruan LMV, Teodora B, Kryssa B, Meiba P, Orlando A, Jayjay M, Sonny C and Vilma V. Phytochemical and anti-bacterial study of Lagerstroemia speciosa (L.) Pers. and its ethnomedicinal importance to indigenous communities of benguuet province, Philippines. Indian Journal of Traditional Knowledge. 2013; 12(3): 379-383.
28. Priya TT, Sabu MC and Jolly CI: Free radical scavenging and anti-inflammatory properties of Lagerstroemia speciosa (L). Inflammatory Pharmacology. 2008; 16: 182-187.
29. Morshed A, Md. Hasibul H, Shakil S, Nahar K, Rahman S, Ferdaudsi D, Hossain T, Ahmad I, Chowdhury MH and Rahmatullah M. Evaluations of Antinociceptive activity of two Bangladeshi medicinal plants, Kalanchoe pinnata (Lam.) Pers. and Lagerstroemia speciosa (L.) Pers. American Eurasian Network of Scientific Information. 2010; 4(2): 193-197.
30. Unno T, Sakane I and Kakuda T. Inhibition of xanthine oxidase by an aqueous extract of banaba leaves (Lagerstroemia speciosa). Journal of the Japanese Society for Food Science and Technology. 2000; 47: 740-743.
31. Prabhu VV, Chidambaranathan N, Nalini G, Venkataraman S, Jayaprakash S and Nagarajan M. Evaluation of Anti Fibrotic Effect of Lagerstroemia spesiosa (L) Pers on Carbon Tetrachloride induced liver fibrosis. Current Pharmaceutical Research. 2010; 1(1): 7-12.
32. Syed J, Rakesh KN, Dileep N, Poornima G, Prashith Kekuda TR and Mukunda S. Total phenolic content and antioxidant activity of seed extract of Lagerstroemia speciosa. Chemical Science Transactions. 2013; 2(1): 75-80.
33. Nayak BS, Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complementary and Alternative Medicine. 2006; 6(1): 1-6.
34. J. B. Harborne, Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis, Chapman and Hall, London, UK, 1973.
35. C. M. Ejikeme, C. S. Ezeonu, and A. N. Eboatu. Determination of physical and phytochemical constituents of some tropical timbers indigenous to Niger Delta Area of Nigeria. European Scientific Journal. 2014; 10(18): 247-270.
36. B. A. Boham and A. R. Kocipai. Flavonoids and condensed Tannins from Leaves of Hawaiian Vaccinium vaticulatum and V. calycinium. Pacific Science. 1994; 48: 458-463.
37. Prasanth D, Padmaja R, Samiulla DS. Effects of certain plants on alpha amylase activity. Fitoterapia. 2001; 72: 179-181.
38. B. O. Obadoni and P. O. Ochuko. Phytochemical studies and comparative efficacy of the crude extracts of some hemostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences. 2002; 8(8): 203–208.
39. Rastogi Archit, Mahalingam Gayathri, Munsuami Punnaga. An invitro investigation into the Mechanism of antidiabetic activity of selected Medicinal plants. International Journal of Drug Development and Research. 2013; 5(3): 221-226.