Author(s):
Manu Mehta, Sarika Gupta, Ajay Duseja
Email(s):
sarika.ashish@gmail.com , manumehta1298@gmail.com , ajayduseja@yahoo.co.in
DOI:
10.52711/0974-360X.2021.00814
Address:
Manu Mehta1, Sarika Gupta2, Ajay Duseja3
1Pursuing PhD, Department of Bioscience Biotechnology, Banasthali Vidyapith, Jaipur, India.
2Department of Biosciences and Biotechnology, Banasthali Vidyapith, Jaipur, India.
3Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 9,
Year - 2021
ABSTRACT:
Background and AIM: Potential utilization of medicine from herbal origin is on the rise due to adverse effects of allopathic medicines. Phyllanthin extracted from Phyllanthus niruri has been shown to be effective in Hepatoprotective effect. Its therapeutic effect in non-alcoholic fatty liver disease (NAFLD) is not much explored. The present study aim was to evaluate the role of phyllanthin in mice fed with methionine choline deficient (MCD) diet. Materials and Methods: Four–five week-old C57lb/6 mice were fed MCD diet for 4 weeks to develop experimental model of NAFLD in small animal research laboratory, PGIMER, Chandigarh. Phyllanthin extract and commercially available phyllanthin was used to assess the impact on NAFLD animal model. After recovery animal weight, liver function test, and lipid profile were measured. Data were showed as mean and standard deviation. Statistical analysis was executing using SPSS v21. Results: Data revealed that administration of 200mg/kg and 400mg/kg crude extract significantly reduced (P<0.0001) bilirubin levels in MCDD mice (0.69±0.14 and 0.85±0.14) respectively. Administration of 400mg/kg phyllanthin significantly reduced SGOT in MCDD mice (23.3±11.87 vs. 50.17±7.68; P=0.002). Administration of 200mg/kg and 400 mg/kg crude extract significantly reduced SGPT in MCDD mice (29.4±14.39 and 21.80±6.12 respectively) (P<0.0001). Similarly, plant phyllanthin improved lipid profile in MCD animals. The plant phyllanthin does not have adverse effect on body weight. Conclusion: Out of the two test trails as plant phyllanthin and commercial phyllanthin, the former suggested hepatoprotective action, as it causes the reversal of damage in the hepatic profile due to NAFLD.
Cite this article:
Manu Mehta, Sarika Gupta, Ajay Duseja. Evaluation of Hepatoprotective effect of Phyllanthus niruri in experimental model of NAFLD. Research Journal of Pharmacy and Technology. 2021; 14(9):4685-0. doi: 10.52711/0974-360X.2021.00814
Cite(Electronic):
Manu Mehta, Sarika Gupta, Ajay Duseja. Evaluation of Hepatoprotective effect of Phyllanthus niruri in experimental model of NAFLD. Research Journal of Pharmacy and Technology. 2021; 14(9):4685-0. doi: 10.52711/0974-360X.2021.00814 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-28
REFERENCES:
1. Duseja A. Nonalcoholic fatty liver disease in India – a lot done, yet more required! Indian J Gastroenterol. 2010; 29: 217–225
2. Iqbal U, Perumpail BJ, Akhtar D, Kim D, Ahmed A. The Epidemiology, Risk Profiling and Diagnostic Challenges of Nonalcoholic Fatty Liver Disease. Medicines. 2019; 6: 41.
3. Yang JD, Abdelmalek MF, Pang H, Guy CD, Smith AD, Diehl AM et al. Gender and Menopause Impact Severity of Fibrosis Among Patients with Nonalcoholic Steatohepatitis. Hepatology 2014, 59, 1406-1414.
4. Yao, Yu-Jie Qiao, Ya-Li Zhao, Xu-Feng Tao et.al. Herbal medicines and nonalcoholic fatty liver disease. Hong World J Gastroenterol 2016; 30: 6890-6905
5. Khanna AK, Rizvi F, Chander R. Lipid lowering activity of Phyllanthus niruri in hyperlipemic mice. J Ethnopharmacol. 2002; 82: 19-22
6. Pearlman FC, Lee RT. Detection and measurement of total bilirubin in serum, with use of surfactants as solubilizing agents. Clin Chem. 1974; 20: 447-53
7. Thomas L. Alanine aminotransferase (ALT), Aspartate aminotransferase (AST). In: Thomas L, editor. Clinical Laboratory Diagnostics. 1st ed. Frankfurt: TH-Books Verlagsgesellschaft; 1998. p. 55-65
8. Bradley DW, Maynard JE, Emery G, Webster H. Transaminase activities in serum of long-term hemodialysis patients. Clin Chem. 1972; 18: 1442
9. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem. 1974; 20: 470-5
10. Rifai N, Bachorik PS, Alberts JJ. Lipids, lipoproteins and apolipoproteins. In: Burtis CA, Ashwood ER, editors Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia: W. Saunders Company; 1999. p. 809-61
11. Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res. 1970; 11: 583-95.
12. Townsend SA, Newsome PN. Non-alcoholic fatty liver disease in 2016. Br Med Bull. 2016; 119: 143–156
13. Ong, H., and Norzalina, J. Malay herbal medicine in Gemencheh, Negri Sembilan, Malaysia. Fitoterapia. 1999; 70: 10-14.
14. C.O. Okoli, I.C. Obidike, A.C. Ezike , P.A. Akah, and O.A. Salawu. Studies on the possible mechanisms of antidiabetic activity of extract of aerial parts of Phyllanthus niruri. Pharmaceutical Biology, 2011; 49: 248-255.
15. Kim S-B, Kang O-H, Lee Y-S, Han S-H, Ahn Y-S, Cha S-W, et al. Hepatoprotective Effect and Synergism of Bisdemethoycurcumin against MCD Diet-Induced Nonalcoholic Fatty Liver Disease in Mice. PLoS ONE 2016; 11: e0147745
16. Van Herck MA, Vonghia L, Francque SM. Animal Models of Nonalcoholic Fatty Liver Disease-A Starter's Guide. Nutrients. 2017; 9: 1072.
17. Al Zarzour RH, Ahmad M, Asmawi MZ, et al. Phyllanthus Niruri Standardized Extract Alleviates the Progression of Non-Alcoholic Fatty Liver Disease and Decreases Atherosclerotic Risk in Sprague-Dawley Mice. Nutrients. 2017; 9: 766.
18. Zahra AA, Mehmet B, Alshawsh MA, Ali HM, Hadi HA, Abdulla MA. Protective Role of Phyllanthus niruri Extract against Thioacetamide-Induced Liver Cirrhosis in Rat Model. Evidence-Based Complementary and Alternative Medicine. 2012: Article ID 241583.
19. Blumberg BS, Millman I, Venkateswaran PS, Thyagarajan SP. Hepatitis B Virus and hepatocellular carcinoma-treatment of HBV carriers with Phyllanthus amarus. Cancer Detect Prevent. 1989; 1.
20. Manjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM , Hegde A et al. Effect of Phyllanthus niruri Linn. Treatment on liver, kidney and testes in CCl4 induced hepatotoxic mice. Indian Journal of Experimental Biology. 2008; 46: 514-520