Haitham Qaralleh, Khaled M Khleifat, Ali M Khlaifat, Muhamad Al-limoun, Nafe M Al-Tawarah, Amir Menwer Alhroob, Ahmad B Alsaudi
Haitham Qaralleh1*, Khaled M Khleifat2, Ali M Khlaifat3, Muhamad Al-limoun4, Nafe M Al-Tawarah1, Amir Menwer Alhroob4, Ahmad B Alsaudi3
1Department of Medical Laboratory Sciences, Mutah University, Mutah, Karak, 61710, Jordan.
2Biology Department, Mutah University, Mutah, Karak, 61710, Jordan.
3Department of Nursing, Faculty of Prince Aysha for Applied Health and Nursing, Al-Hussein Bin Talal University, Ma'an, Jordan.
4Department of Laboratory Medical Sciences, Princess Aisha Bent Al-Hussein, Faculty of Nursing and Health Sciences, Al Hussein bin Talal University, Jordan.
Volume - 14,
Issue - 9,
Year - 2021
The Jordanian endemic medicinal plant, Cupressus sempervirens was obtained from Dhana Natural Reserve, Al-Tafilah, Jordan to investigate its antibacterial and antioxidant activities. The procuring of essential oil was made by processing of dry leaves of C. sempervirens using steam-distillation method giving 0.26% (w/w) yield. The analysis of obtained EO for its chemical constituents, was achieved by GC-MS. The equivalent of 94.02% of the entire EO has been extracted and consists of twenty-two compounds. The characterization of EO was made by their presence of three groups of chemical compounds namely Sesquiterpene hydrocarbons (71.0%), Oxygenated sesquiterpenes (11.5%) and Monoterpenes hydrocarbons (10.6%). The major constituent was germacrene-D (14.2%) along with the d-cadinene (11.0%), ß-pinene (10.0%) and isocedrol (9.8%). This conferring a chemotaxonomic value as well as a higher degree of polymorphism in the occurrence of these compounds in C. sempervirens as compared with the same species in different location worldwide. The efficiency of methanol extract and essential oil as antibacterial was evaluated against nine bacteria, using disc diffusion and MIC methods. Results showed that the methanol extract at 2000µg/disc of C. sempervirens caused the growth inhibition of Bacillus subtilis, Micrococcus luteus, Staphylococcus epidermidis and Staphylococcus aureus and producing inhibition zone ranges between 12-15mm. The MIC values recorded by essential oils of C. sempervirens were as follow: S. epidermidis and S. aureus (370µg/mL), Escherichia coli and Enterobacter aerogenes (1000µg/mL), Pseudomonas aeruginosa (2000µg/mL) and M. luteus, Klebsiella pneumonia and Salmonella typhi (3000µg/mL). In the present study, C. sempervirens extract exerted antioxidant efficiency with an IC50 value of 27.31µg/mL.
Cite this article:
Haitham Qaralleh, Khaled M Khleifat, Ali M Khlaifat, Muhamad Al-limoun, Nafe M Al-Tawarah, Amir Menwer Alhroob, Ahmad B Alsaudi. Chemical Composition, Antioxidant and Inhibitory Effect of Cupressus sempervirens Essential Oils and Methanolic Extract on Beta-lactamase producing Isolates. Research Journal of Pharmacy and Technology. 2021; 14(9):4673-9. doi: 10.52711/0974-360X.2021.00812
Haitham Qaralleh, Khaled M Khleifat, Ali M Khlaifat, Muhamad Al-limoun, Nafe M Al-Tawarah, Amir Menwer Alhroob, Ahmad B Alsaudi. Chemical Composition, Antioxidant and Inhibitory Effect of Cupressus sempervirens Essential Oils and Methanolic Extract on Beta-lactamase producing Isolates. Research Journal of Pharmacy and Technology. 2021; 14(9):4673-9. doi: 10.52711/0974-360X.2021.00812 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-26
1. Homady MH, Khleifat KM, Tarawneh KA, et al. Reproductive toxicity and infertility effect of Ferula hormonis extracts in mice. Theriogenology. 2002; 57(9): 2247–2256.
2. Zhang X. Traditional medicine: its importance and protection. Prot. Promot. Tradit. Knowl. Syst. Natl. Exp. Int. Dimens. Part. 2004; 1: 3–6.
3. Tarawneh KA, Irshaid F, Jaran AS, et al. Evaluation of antibacterial and antioxidant activities of methanolic extracts of some medicinal plants in northern part of Jordan. J. Biol. Sci. 2010; 10(4): 325–332.
4. Joshi RK. Chemical Constituents of Artemisia nilagirica (Clarke) from Western Himalaya of Uttarakhand, India. Asian J. Pharm. Anal. 2020; 10(4): 182–184.
5. Althunibat OY, Al-Mustafa AH, Tarawneh K, et al. Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats. Process Biochem. 2010; 45(4): 581–585.
6. Mariyappan M, Bharathidasan R, Mahalingam R, et al. Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla. Asian J. Pharm. Res. 2011; 1(4): 111–113.
7. Paul S, Saha D. Comparative Study of the Efficacy of Barleria prionitis Leaf extracts against Bacteria. Asian J. Pharm. Res. 2012; 2(3): 107–110.
8. Khleifat K, Homady MH, Tarawneh KA, et al. Effect of Ferula hormonis extract on social aggression, fertility and some physiological parameters in prepubertal male mice. Endocr. J. 2001; 48(4): 473–482.
9. Althunibat OY, Qaralleh Q, Al-Dalin SYA, et al. Effect of Thymol and Carvacrol, the Major Components of Thymus capitatus on the Growth of Pseudomonas aeruginosa. J. Pure Appl. Microbiol. 2016; 10(1): 367–374.
10. Tellawi AM, Prescott MJ, Ayesh EM, et al. Conservation and sustainable use of biological diversity in Jordan. GCEP, Amman, Jordan. 2001; 118.
11. Cragg GM, Newman DJ, Snader KM. Natural products in drug discovery and development. J. Nat. Prod. 1997; 60(1): 52–60.
12. Tarawneh KA, Al‐Tawarah N, Abdel‐Ghani AH, et al. Characterization of verotoxigenic Escherichia coli (VTEC) isolates from faeces of small ruminants and environmental samples in Southern Jordan. J. Basic Microbiol. 2009; 49(3): 310–317.
13. Izzo AA, Di Carlo G, Biscardi D, et al. Biological screening of Italian medicinal plants for antibacterial activity. Phyther. Res. 1995; 9(4): 281–286.
14. Sacchetti G, Maietti S, Muzzoli M, et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem. 2005; 91(4): 621–632.
15. Qaralleh HN, Abboud MM, Khleifat KM, et al. Antibacterial activity in vitro of Thymus capitatus from Jordan. Pak. J. Pharm. Sci. 2009; 22(3).
16. Rammo RNN. Bactericidal and Anti-biofilm Formation of Aqueous Plant Extracts against Pathogenic Bacteria. Asian J. Pharm. Res. 2017; 7(1): 25–29.
17. Qaralleh HN. Chemical composition and antibacterial activity of Origanum ramonense essential oil on the β-lactamase and extended-spectrum β-lactamase urinary tract isolates. Bangladesh J. Pharmacol. 2018; 13(3): 280–286.
18. Tawaha KA, Alali FQ, Hudaib MM. Chemical composition and general cytotoxicity evaluation of essential oil from the flowers of Anthemis palestina Reut. ex Boiss., growing in Jordan. J. Essent. Oil Bear. Plants. 2015; 18(5): 1070–1077.
19. Kokate CK. A Textbook for Practical Pharmacognosy. edn. 2005.
20. Qaralleh H, Khleifat KM, Al-Limoun MO, et al. Antibacterial and synergistic effect of biosynthesized silver nanoparticles using the fungi Tritirachium oryzae W5H with essential oil of Centaurea damascena to enhance conventional antibiotics activity. Adv. Nat. Sci. Nanosci. Nanotechnol. 2019; 10(2): 25016.
21. Qaralleh H, Idid S, Saad S, et al. Antifungal and antibacterial activities of four Malaysian sponge species (Petrosiidae). J. Mycol. Médicale/Journal Med. Mycol. 2010; 20(4): 315–320.
22. Dudonné S, Vitrac X, Coutiere P, et al. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J. Agric. Food Chem. 2009; 57(5): 1768–1774.
23. Karahan F, Avsar C, Ozyigit II, et al. Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats. Biotechnol. Biotechnol. Equip. 2016; 30(4): 797–804.
24. Rahmani Z, Douadi A, Rahmani Z. in vitro inhibition of a-amylase enzyme, phytochemical study and antioxidant capacity for cupressus sempervirens extracts growing in arid climat. Asian J. Res. Chem. 2019; 12(6): 359–365.
25. Zengin G, Cakmak YS, Guler GO, et al. In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from Central Anatolia region of Turkey. Food Chem. Toxicol. 2010; 48(10): 2638–2641.
26. Tadros MJ, Alqudah AM, Arabiat YS. Comparative study between Cupressus sempervirens and Cupressus arizonica in seed germination and seedling vigour. Crop Res. 2010; 40(1/3): 174–181.
27. Chandrasekaran S, Geetha R V. Antibacterial Activity of the Three Essential Oils on Oral Pathogens-An In-vitro Study. Res. J. Pharm. Technol. 2014; 7(10): 1128–1129.
28. Uçar G, Uçar MB, Fakir H. Composition of volatile foliage isolates from Cupressus sempervirens varieties (Var. horizontal Mill. and pyramidalis Nyman) growing in Turkey. J. Essent. Oil Res. 2007; 19(6): 562–565.
29. Elansary HO, Salem MZM, Ashmawy NA, et al. Chemical composition, antibacterial and antioxidant activities of leaves essential oils from Syzygium cumini L., Cupressus sempervirens L. and Lantana camara L. from Egypt. J. Agric. Sci. 2012; 4(10): 144.
30. Rguez S, Djébali N, Slimene I Ben, et al. Cupressus sempervirens essential oils and their major compounds successfully control postharvest grey mould disease of tomato. Ind. Crops Prod. 2018; 123: 135–141.
31. Selim SA, Adam ME, Hassan SM, et al. Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.). BMC Complement. Altern. Med. 2014; 14(1): 179.
32. Zhang J, Rahman AA, Jain S, et al. Antimicrobial and antiparasitic abietane diterpenoids from Cupressus sempervirens. Res. reports Med. Chem. 2012; 2012(2): 1–6.
33. Boukhris M, Regane G, Yangui T, et al. Chemical composition and biological potential of essential oil from Tunisian Cupressus sempervirens L. J. Arid L. Stud. 2012; 22(1): 329–332.
34. Chaudhary HJ, Shahid W, Bano A, et al. In vitro analysis of Cupressus sempervirens L. plant extracts antibaterial activity. J. Med. Plants Res. 2012; 6(2): 273–276.
35. Tumen I, Senol FS, Orhan IE. Evaluation of possible in vitro neurobiological effects of two varieties of Cupressus sempervirens (Mediterranean cypress) through their antioxidant and enzyme inhibition actions. Turkish J. Biochem. Biyokim. Derg. 2012; 37(1).
36. Mahmood Z, Ahmed I, Saeed MUQ, et al. Investigation of physico-chemical composition and antimicrobial activity of essential oil extracted from lignin-containing Cupressus sempervirens. BioResources. 2013; 8(2): 1625–1633.
37. Hassanzadeh Khayyat M, Emami SA, Rahimizadeh M, et al. Chemical constituents of Cupressus sempervirens L. cv. Cereiformis Rehd. essential oils. Iran. J. Pharm. Sci. 2005; 1(1):3 9–42.
38. Al-Asoufi A, Khlaifat A, Al Tarawneh A, et al. Bacterial Quality of Urinary Tract Infections in Diabetic and Non Diabetics of the Population of Ma’an Province, Jordan. Pak. J. Biol. Sci. 2017; 20(4): 179–188.
39. Khleifat KM, Abboud MM, Al-Mustafa AH, et al. Effects of carbon source and Vitreoscilla hemoglobin (VHb) on the production of β-galactosidase in Enterobacter aerogenes. Curr. Microbiol. 2006; 53(4): 277–281.
40. Mazari K, Bendimerad N, Bekhechi C. Chemical composition and antimicrobial activity of essential oils isolated from Algerian Juniperus phoenicea L. and Cupressus sempervirens L. J. Med. Plants Res. 2010; 4(10): 959–964.
41. Al-Snafi AE. Medical importance of Cupressus sempervirens-A review. IOSR J. Pharm. 2016; 6(6): 66–76.
42. Toroglu S. In vitro antimicrobial activity and antagonistic effect of essential oils from plant species. J. Environ. Biol. 2007; 28(3): 551–559.
43. Mamtha B, Kavitha K, Srinivasan KK, et al. An in vitro study of the effect of Centella asiatica [Indian pennywort] on enteric pathogens. Indian J. Pharmacol. 2004; 36(1): 41.
44. Tiwari P, Patel RK. Estimation of total phenolics and flavonoids and antioxidant potential of Ashwagandharishta prepared by traditional and modern methods. Asian J. Pharm. Anal. 2013; 3(4): 147–152.
45. Cowan MM. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999; 12(4): 564–582.
46. Tiwari P. Phenolics and flavonoids and antioxidant potential of balarishta prepared by traditional and modern methods. Asian J. Pharm. Anal. 2014; 4(1): 5–10.
47. Epand RF, Savage PB, Epand RM. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochim. Biophys. Acta (BBA)-Biomembranes. 2007; 1768(10): 2500–2509.
48. Krishna K V, Karuppuraj V, Perumal K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Anal. 2016; 6(4): 213–215.