Author(s): Hiral Vasavada, Sailaja Inampudi

Email(s): vasavadahiral74@gmail.com , inampudisailaja@rediffmail.com

DOI: 10.52711/0974-360X.2021.00810   

Address: Ms. Hiral Vasavada1, Dr. Sailaja Inampudi2
1Ph.D Scholar, Department of Biotechnology, Parul University of Applied Sciences, Parul University, Limda, Waghodia, Vadodara, Gujarat - 391769, India.
2Faculty of Department of Biotechnology, Parul University of Applied Sciences, Parul University, Limda, Waghodia, Vadodara, Gujarat - 391769, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021


ABSTRACT:
Different plant extracts are considerably safe from infectious agents and may be used for medical purposes. The present research was conducted against the six standard microorganisms to quantify the antimicrobial activities of peppermint (Mentha piperita) extracts. The traditional approaches of minimum bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) were used to approximate the antibacterial activities of ethanol, methanol, and chloroform peppermint extracts. The inhibitory function of the ethanol extract was comparable to that of chloroform (10 to 80mg/ml) and methanol (10 to 80mg/ml) against all gram-negative microorganisms. The minimum value of MIC was recorded for Streptococcus pyogenes (5mg/ml for extract of ethanol), followed by E. coli (10mg/ml for extract of ethanol) and then by Enterococcus faecalis (15mg/ml for extract of ethanol). With respect to the standard microorganisms, the MBC values were higher for both extracts than the corresponding MIC values. This work demonstrated the possible efficacy of antibacterial action on M. Piperita extracts from normal microorganisms (A. Baumenii, Escherichia coli, Streptococcus pyogenes, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsiella pneumoniae), particularly ethanol extract. In summary, the peppermint ethanol extract had important growth-inhibiting effects on observed standard micro-organisms, followed by chloroform and methanol extracts. Further to in vitro and in vivo studies on a wide variety of natural microorganisms and therapeutic isolates are required to investigate and standardize the inhibitory activity of peppermint extracts against the most dangerous human pathogenic agents.


Cite this article:
Hiral Vasavada, Sailaja Inampudi. Minimal Inhibitory Concentration and Minimal Bactericidal Concentration of Peppermint (Mentha piperita) extracts against standard microorganisms. Research Journal of Pharmacy and Technology. 2021; 14(9):4662-6. doi: 10.52711/0974-360X.2021.00810

Cite(Electronic):
Hiral Vasavada, Sailaja Inampudi. Minimal Inhibitory Concentration and Minimal Bactericidal Concentration of Peppermint (Mentha piperita) extracts against standard microorganisms. Research Journal of Pharmacy and Technology. 2021; 14(9):4662-6. doi: 10.52711/0974-360X.2021.00810   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-24


REFERENCES:
1.    Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C. Antibiotic resistance—the need for global solutions. The Lancet infectious diseases. 2013; 13(12): 1057-98.
2.    Allcock S, Young EH, Holmes M, Gurdasani D, Dougan G, Sandhu MS, Solomon L, Török ME. Antimicrobial resistance in human populations: challenges and opportunities. Global Health, Epidemiology and Genomics. 2017; 2.
3.    Uchil RR, Kohli GS, Kate Khaye VM, Swami OC. Strategies to combat antimicrobial resistance. Journal of clinical and diagnostic research: JCDR. 2014; 8(7): ME01.
4.    Goldberg AM, Hartung T. Protecting more than animals. Scientific American. 2006; 294(1) :84-91.
5.    Laxminarayan R. How broad should the scope of antibiotics patents be?. American Journal of Agricultural Economics. 2002; 84(5): 1287-92.
6.    Horrigan L, Lawrence RS, Walker P. How sustainable agriculture can address the environmental and human health harms of industrial agriculture. Environmental health perspectives. 2002; 110(5): 445-56.
7.    Kohanski MA, Dwyer DJ, Collins JJ. How antibiotics kill bacteria: from targets to networks. Nature Reviews Microbiology. 2010; 8(6): 423-35.
8.    George J. Bioactive Screening and Antimicrobial Activity of Selected Three Medicinal Plants on Chosen Microbes. Research Journal of Pharmacy and Technology. 2014; 7(11): 1264-9.
9.    Anup K, Mohan K, Suraj S, Sandip F, Bhushan F, Prashant W. Antimicrobial Activity of Some Important Medicinal Plants of India against Some Plant and Human Pathogens. Research Journal of Pharmacy and Technology. 2010; 3(3): 924-6.
10.    Hindi NK, Abdul-Husin IF, Al-Mahdi ZK, Ewadh RM, Hossain AO, Kadhim MJ, Alnasraui AH, Al-Yaseri AA. Effectiveness of Aqueous extract of Green, Black and Red Tea Leaves against some types of Gram positive and negative bacteria. Research Journal of Pharmacy and Technology. 2017; 10(6): 1957-62.
11.    Singh R, Shushni MA, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry. 2015; 8(3): 322-8.
12.    Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML, Senthilkumar M, Sathya K, Sreeramanan S. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). Journal of Medicinal Plants Research. 2012 Jan 16; 6(2): 331-5.
13.    Jeyakumar E, Lawrence R, Pal T. Comparative evaluation in the efficacy of peppermint (Mentha piperita) oil with standards antibiotics against selected bacterial pathogens. Asian Pacific Journal of Tropical Biomedicine. 2011; 1(2): S253-7.
14.    Bupesh G, Amutha C, Nandagopal S, Ganeshkumar A, Sureshkumar P, Murali K. Antibacterial activity of Mentha piperita L. (peppermint) from leaf extracts-a medicinal plant. Acta Agriculturae Slovenica. 2007; 89(1): 73.
15.    Sarbeen JI. Preliminary phytochemical analysis of Peppermint Oil and Tulsi Oil. Research Journal of Pharmacy and Technology. 2015; 8(7): 929-31.
16.    Nuraskin CA, Idroes R, Soraya C. Study of Inhibition of Methanol Extract of Laban Leaf (Vitex Pinnata) against Sreptococcus mutans with Microdilution. Research Journal of Pharmacy and Technology. 2019; 12(12): 6037-40.
17.    Nair DU, Saraswathy MP, Kishore N, Pully NR. Phytochemical and Antimicrobial Evaluation of Luffa cylindrica Linn. Leaf and Flower Extracts–An In-Vitro Study. Research Journal of Pharmacy and Technology. 2010; 3(2): 438-41.
18.    Thilagavathi T, Kathiravan G. Phytochemical Analysis and Antimicrobial Activity of Ethanolic Leaf Extract of Ficus racemosa Linn. Research Journal of Pharmacy and Technology. 2017; 10(2): 537-40.
19.    Anowi CF, Cardinal NC, Ike C, Ezeokafor E. Antimicrobial properties of the ethyl acetate extract of the stem bark of Nauclea latifolia. Int. J. Adv. Pharm. Biol. Chem. 2012; 1: 183-91.
20.    Sharma H, Karnwal A. Impact of herbal extracts in biocontroling of four human pathogenic bacteria-an in-vitro study. Research Journal of Pharmacy and Technology. 2018; 11(7): 2895-900.
21.    Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evidence-Based Complementary and Alternative Medicine. 2016; 2016.
22.    Harfouch RM, Darwish M, Al-Asadi W, Mohammad AF, Gharib NM, Haroun M. Antibacterial Activity of Essential Oils of Rosmarinus officinalis, Salvia officinalis and Anthemis nobilis Widespread in the Syrian Coast. Research Journal of Pharmacy and Technology. 2019; 12(7): 3410-2.
23.    Andoğan BC, Baydar H, Kaya S, Demirci M, Özbaşar D, Mumcu E. Antimicrobial activity and chemical composition of some essential oils. Archives of Pharmacal research. 2002; 25(6): 860-4.
24.    Thosar N, Basak S, Bahadure RN, Rajurkar M. Antimicrobial efficacy of five essential oils against oral Pathogens: An in vitro study. European Journal of Dentistry. 2013; 7(S 01): S071-7.
25.    Castillo-Juárez I, González V, Jaime-Aguilar H, Martínez G, Linares E, Bye R, Romero I. Anti-Helicobacter pylori activity of plants used in Mexican traditional medicine for gastrointestinal disorders. Journal of Ethnopharmacology. 2009; 122(2): 402-5.
26.    Zaidi S, Dahiya P. In vitro antimicrobial activity, phytochemical analysis and total phenolic content of essential oil from Mentha spicata and Mentha piperita. International Food Research Journal. 2015; 22(6): 2440.
27.    Chao SC, Young DG, Oberg CJ. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research. 2000; 12(5): 639-49.
28.    Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine. 2006; 6(1): 39.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available