Nadar Sowmya, Chouhan Raghavendra Singh, Kosha Patel, Harshil Patel, Tanvi Dodiya, Muthu Kumaradoss Mohan Maruga Raja
email@example.com , firstname.lastname@example.org , email@example.com , firstname.lastname@example.org , email@example.com , firstname.lastname@example.org
Nadar Sowmya, Chouhan Raghavendra Singh, Kosha Patel, Harshil Patel, Tanvi Dodiya, Muthu Kumaradoss Mohan Maruga Raja*
Department of Pharmacognosy and Phytochemistry, Parul Institute of Pharmacy and Research, Parul University, Vadodara, Gujarat, India.
Volume - 14,
Issue - 9,
Year - 2021
According to the literature survey most of the studies done on Adarak (Zingiber officinale rhizome) were performed on alcoholic extracts or isolated entities of ginger but no profound work has been done on the traditionally prepared or commonly consumed way of ginger. So, in this current study, fresh ginger rhizomes were traditionally pounded to make Adarak juice. The different concentration (0.05%, 0.5%, 1%, 2.5%, 5%, 10%) were taken as sample to perform onion root tip inhibition assay and brine shrimp lethality bioassay. It showed antimitotic inhibition in the range of 0.05-10% concentration with an IC50 value of 0.37 % on number of rootlets was 0.08 % on length of rootlets. The traditionally prepared Adarak juice showed cytotoxicity in the range of 0.05-2.5% concentration with LC50 value of 1.59%. The traditionally prepared Adarak juice possesses cytotoxic and antimitotic activity.
Cite this article:
Nadar Sowmya, Chouhan Raghavendra Singh, Kosha Patel, Harshil Patel, Tanvi Dodiya, Muthu Kumaradoss Mohan Maruga Raja. In-vitro and in vivo studies of traditionally prepared Zingiber officinale juice for Antimitotic and Lethality activity. Research Journal of Pharmacy and Technology. 2021; 14(9):4652-6. doi: 10.52711/0974-360X.2021.00808
Nadar Sowmya, Chouhan Raghavendra Singh, Kosha Patel, Harshil Patel, Tanvi Dodiya, Muthu Kumaradoss Mohan Maruga Raja. In-vitro and in vivo studies of traditionally prepared Zingiber officinale juice for Antimitotic and Lethality activity. Research Journal of Pharmacy and Technology. 2021; 14(9):4652-6. doi: 10.52711/0974-360X.2021.00808 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-22
1. Jaiswal YS and Williams LL. A glimpse of Ayurveda – The forgotten history and principles of Indian traditional medicine. Journal of Traditional and Complementary Medicine. 7 (1); 2017: 50-53.
2. Patwardhan B and Khambholja K. Drug discovery and Ayurveda: Win-win relationship between contemporary and ancient sciences. In Drug discovery and development – Present and Future, Edited by Kapetanovic I. In Tech, Croatia. 2011; 1st ed: pp. 9-10.
3. Jainab NH and Mohan Maruga Raja MK. In silico molecular docking studies on the chemical constituents of Clerodendrum phlomidis for its cytotoxic potential against breast cancer markers. Research Journal of Pharmacy and Technology. 11(4); 2018: 1612-1618.
4. Cardellina JH, Fuller RW, Gamble WR, Westergaard C, Boswell J, Munro MHG, et al. Evolving strategies for the selection dereplication and prioritization of antitumor and HIV inhibitory natural products extracts. In Bioassay methods in natural product research and development, Edited by Bohlin L and Bruhn JG. Kluwer Academic Publishers, Dordrecht. 1999: pp. 25-36.
5. Vedashree M, Asha MR, Roopavati C and Naidu MM. Characterization of volatile components from ginger plant at maturity and its value addition to ice cream. Journal of Food Science and Technology. 57; 2020: 3371–3380.
6. Nair KP. Turmeric (Curcuma longa L.) and Ginger (Zingiber officinale Rosc.) - World's invaluable medicinal spices. Springer Nature, Switzerland. 2019.
7. Markam R and Bajpai AK. Functionalization of ginger derived nanoparticles with chitosan to design drug delivery system for controlled release of 5-amino salicylic acid (5-ASA) in treatment of inflammatory bowel diseases: An in vitro study. Reactive and functional polymers. 149; 2020: 104520.
8. Singh CB, Manglembi N, Swapana N and Chanu SB. Ethnobotany, phytochemistry and pharmacology of Zingiber cassumunar Roxb. (Zingiberaceae). Journal of Pharmacognosy and Phytochemistry. 4 (1); 2015: 1-6.
9. Kizhakkayil J and Sasikumar B. Diversity, characterization and utilization of ginger: A review. Plant Genetic Resources. 9 (3); 2011: 464-477.
10. Rajurkar RM, Jain RG, Bedmohta PA, Khadbadi SS. Antioxidant activity of phenolic extract from Ginger (Zingiber officinale Roscoe) Rhizome. Asian J. Research Chem. 2(3); 2009: 260-261.
11. Debjit B, Chiranjib P, Tripathi KK, Chandira MR, Sampath Kumar KP. Zingiber officinale, the herbal and traditional medicine and its therapeutically importance. Research J. Pharmacognosy and Phytochemistry. 2(2); 2010: 102-110.
12. Susanta R, Saptarshi D, Bhabagrahi R. Evaluation of broncho-protective effect of Zingiber officinale Roscoe in histamine induced broncho-spasm. Research J. Pharm. and Tech. 3(2); 2010: 589-591.
13. Sivakumar M, Srikanth J. In-silico and in-vitro evaluation of xanthine oxidase inhibition of Zingiber officinale for hypouricemic activity. Research J. Pharm. and Tech. 2019; 12(1): 314-320.
14. Vaishali. RU, Asawari ST, Pooja VK. Combination of Zingiber officinale and Brassica oleraceae extracts shows better anti-arthritic activity. Research J. Pharm. and Tech. 2020; 13(3): 1175-1178.
15. Ndanusa AH, Rohini K, Uma Sankar A, Khin Mar A. Effect of Zingiber officinale on lipid profile of Sprague-Dawley rats induced with streptozotocin. Research J. Pharm. and Tech. 2020; 13(8): 3577-3579.
16. Padmavathi P, Raja Sankar, Kokilavani N. A study to assess the effectiveness of ginger powder on dysmenorrhoea among adolescents in a selected school at erode. Asian J. Nur. Edu. & Research. 2012; 2(2): 79-82.
17. Devangamath BG, Raddi SA. A randomized control trial to assess the efficacy of dry ginger powder on management of nausea and vomiting among antenatal mothers attending selected urban health centres of Belgaum, Karnataka – one group pretest posttest pre experimental study. Asian J. Nur. Edu. and Research. 2016; 6(1): 17-26.
18. Camargo JNA, Bertan AS, Almeida IV, Vicentini VEP, Dusman E and Tonin LTD. Antitumoral activity, antioxidant capacity and bioactive compounds of ginger (Zingiber officinale). Acta Scientiarum: Technology. 42; 2019: e45724.
19. Jeena K, Liju VB and Kuttan R. Antitumor and cytotoxic activity of ginger essential oil (Zingiber officinale roscoe). International Journal of Pharmacy and Pharmaceutical Science. 7 (8); 2015: 341-344.
20. Ashraf SM, Sebastian J and Rathinasamy K. Zerumbone, a cyclic sesquiterpene, exerts antimitotic activity in HeLa cells through tubulin binding and exhibits synergistic activity with vinblastine and paclitaxel. Cell proliferation in basic and clinical science. 52 (2); 2018: e12558.
21. Iqbal J, Abbasi BA, Mahmood T, Kanwal S, Ali B, Shah SA and Khalil AT. Plant-derived anticancer agents: A green anticancer approach. Asian Pacific Journal of Tropical Biomedicine. 7 (12); 2017: 1129-1150.
22. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE and McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 45 (5); 1982: 31-34.
23. Sarah QS, Anny FC and Misbahuddin M. Brine shrimp lethality assay. Bangladesh Journal of Pharmacology. 12; 2017: 186-189.
24. Asaduzzaman M, Rana MS, Raqibul Hasan SM, Hossain MM and Das N. Cytotoxic (brine shrimp lethality bioassay) and antioxidant investigation of Barringtonia acutangula (L.). International Journal of Pharma Sciences and Research. 6 (8); 2015: 1179-1185.
25. Lokanatha Rai RM, Basavaraju YB and Sadashivamurthy B. Biological assay and antimitotic activity of novel analogues of β-apopicropodophyllin. Indian Journal of Pharmaceutical Sciences. 69 (1); 2007: 116-118.
26. Devarajan A and Mohan Maruga Raja MK. Cytotoxic, antimitotic, and antiproliferation studies on Rasam: A South Indian traditional functional food. Pharmacognosy Magazine. 13 (Suppl 3); 2017: S452–57.
27. Khanna N and Sharma S. Allium Cepa root chromosomal aberration assay: A review. Indian Journal of Pharmaceutical and Biological Research. 1 (3); 2013: 105-109.
28. Patil NS, Patil KB, Patil MR, Ahirrao RA. Antimitotic Activity of fruits of Momordica dioica by using Allium cepa root tip assay. Asian Journal of Pharmaceutical Research. 8 (4); 2018: 221-224.
29. Devarajan A, Mohan Maruga Raja MK. Identification and analysis of chemical constituents of rasam by gas chromatography–mass spectrometry (GC-MS). Research Journal of Pharmacy and Technology. 10 (12); 2017: 4183-4187.