Author(s): S. Sharmila, S. Preetha, E. Kowsalya, R. Kamalambigeswari, L. Jeyanthi Rebecca

Email(s): sharu312@gmail.com

DOI: 10.52711/0974-360X.2021.00796   

Address: S. Sharmila*, S. Preetha, E. Kowsalya, R. Kamalambigeswari, L. Jeyanthi Rebecca
Dept. of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India – 600073.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021


ABSTRACT:
Enzymes are biological molecules that significantly speed up the rate of virtually all of the chemical reactions that takes place within the cells. They are vital for life and serve as a wide range of important functions in the body. Solid state fermentation holds a high potential for the production of enzyme amylase using Aspergillus niger. In this work, different solid substrates such as groundnut shells, coconut coir and Palmyra sprout peels were used for the production of amylase as they are very cheap and also easily available raw materials. Then the maximum enzyme activities were analysed. Results showed that the enzyme activity of for which palmyra sprout peel was used as substrate had maximum activity in both crude sample (0.63µmol/ml.min) and in partially purified sample (1.42µmol/ml.min) and activity was found to be less for groundnut shell as substrate (crude sample 0.36µmol/ml.min) and in (treated sample 0.26µmol/ml.min) and also the specific activity was found to be maximum in palmyra sprout peel (29.2U/mg of protein) and less in groundnut shell (8.6U/mg of protein).`


Cite this article:
S. Sharmila, S. Preetha, E. Kowsalya, R. Kamalambigeswari, L. Jeyanthi Rebecca. Fermentative extraction of Amylase using waste Biomaterials as Substrate. Research Journal of Pharmacy and Technology. 2021; 14(9):4580-2. doi: 10.52711/0974-360X.2021.00796

Cite(Electronic):
S. Sharmila, S. Preetha, E. Kowsalya, R. Kamalambigeswari, L. Jeyanthi Rebecca. Fermentative extraction of Amylase using waste Biomaterials as Substrate. Research Journal of Pharmacy and Technology. 2021; 14(9):4580-2. doi: 10.52711/0974-360X.2021.00796   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-9-10


REFERENCES:
1.    Sharmila S, Jeyanthi Rebecca L, Merina Paul Das and Md Saduzzaman. Isolation and partial purification of Protease from plant leaves. Journal of Chemical and Pharmaceutical Research. 2012; 4(8): 3808-3812.
2.    Sharmila S, Jeyanthi Rebecca L, Susithra G, Dhanalakshmi V and Saduzzaman. Partial Purification of Protease from Seaweed. International Journal of Applied Biotechnology and Biochemistry. 2012; 2(1): 81-85.
3.    Kamalambigeswari R, Sharmila S, Kowsalya E, Selva Janani S, Deva V, Jeyanthi Rebecca L. Lipase production from Enterobacter Sp. Research J. Pharm. and Tech 2019; 12(9): 4417-4420.
4.    Priyanka S, Jayashree M, Shivani R, Anwesha S, Bhaskara Rao K. V. A Study on Marine Lipolytic Yeasts: Optimization and Characterization of Lipase Enzyme. Research J. Pharm. and Tech 2018; 11(2): 593-598
5.    Ashwini K, Shanmugamsampath Kumar. Partial-purification of Alpha-Amylase from Marine Streptomyces gancidicus-ASD_KT852565 . Research J. Pharm. and Tech. 2016; 9(6): 731-734.
6.    Monisha V, Preethika G, Subathra Devi C. Screening for Streptokinase producing Streptococcus sp. from Food and Soil samples. Research J. Pharm. and Tech 2018; 11(7): 2845-2847
7.    Archana L, Madhura Shevkar, Merlyn Keziah S, Subathra Devi C. Extraction of Serralysin: A Fibrinolytic enzyme from Serratia sp. isolated from soil. Research J. Pharm. and Tech 2018; 11(7): 2911-2913
8.    Thiruvengadam S, Jeevanantham S, Kamalesh R, Hamsini S, Kamali T. B, Karishma S, Jayalakshmi H. Designing and Development of Rice water based crude media and it’s application in fungal isolation and enzyme production. Research J. Pharm. and Tech 2018; 11(9): 3905-3908.
9.    Viswanathan K, Jeyanthi Rebecca L. Screening of Marine Actinomycetes for Fibrinolytic Enzymes. Research J. Pharm. and Tech 2018; 11(10): 4365-4369.
10.    Sowmya Hari. Screening of Enzymes from Actinomycetes and Fungi isolated from Plastic Dumped Soil. Research J. Pharm. and Tech. 2019; 12(5): 2261-2266.
11.    Viswanathan K, Jeyanthi Rebecca L. Screening of Amylase and Cellulase Enzymes from Marine Actinomycetes. Research J. Pharm. and Tech 2019; 12(8): 3787-3790.
12.    Lakshmi Priya S, Krishna Prema K. Screening and Isolation of Fibrinolytic Enzymes from Bacteria using Agro-waste for Thrombolytic Treatment. Research J. Pharm. and Tech. 2019; 12(4): 1963-1966
13.    Reeta Rani, Anil Kumar, Carlos R, Soccol Ashok Pandey. Recent advances in solid state fermentation, Biochem. Eng. J. 2009; 44(1): 13-18.
14.    Mitchell DA, and Losane BK. Definition, characterization and economic evaluation, in: H.W Doelle, C. Rolz (Eds), General principles of solid substrate fermentation, Rapid Publications of Oxford Ltd., UK.
15.    Damien M, Catherine J, Patrice D, Christopher B. Enhanced mechanical properties of partially beta-amylase trimmed starch for material application. Carbohydr. Poly. 2010; 80(3): 747-752
16.    Gupta R, Gigras P, Mohapatra H, Goswami VK and Chauhan B. Microbial α-amylases: :a biotechnological perspective. Process Biochem. 2003; 38: 1599 - 1616.
17.    Couto SR, and Sanromán MA. Application of solid-state fermentation to food industry- A review. Journal of Food Engineering. 2006; 76: 291-302.
18.    Gangadharan D, Sivaramakrishnan S, Nampoothiri KM, Sukumaran RK and Pandey A. Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens. Bioresour Technol. 2008; 99: 4597-4602.
19.    Bradford, MM. A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976; 72: 248-254.
20.    Dibyangana Raul, Tania Biswas, Suchita Mukhopadhyay, Shrayan Kumar Das, and Suvroma Gupta. Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) using solid state fermentation. Biochemistry Research International. 2014, 1-5.

Recomonded Articles:

Author(s): Basanti Majhi, Kunja Bihari Satapathy, Sagar Kumar Mishra

DOI: 10.5958/0974-360X.2019.00202.6         Access: Open Access Read More

Author(s): A. Khuntia, S.K. Mohanty

DOI: Not Available         Access: Open Access Read More

Author(s): Vaishvedhidha M.U, Ezilrani. P, Godwin Christopher. J

DOI: 10.5958/0974-360X.2015.00255.3         Access: Open Access Read More

Author(s): N. Raaman, S. Selvarajan, D. Balakrishnan, G. Balamurugan

DOI:         Access: Open Access Read More

Author(s): Govindasamy Vinodhini, Madhava Anil Kumar, Saptharishi Balamanikandan, Muthulingam Seenuvasan

DOI: 10.5958/0974-360X.2015.00010.4         Access: Open Access Read More

Author(s): Ravula Nanditha, Saravanan J, Praveen T K, Deepa S, Emdormi Rymbai

DOI: 10.5958/0974-360X.2020.00733.7         Access: Open Access Read More

Author(s): Ajay Raghuvanshi, D.M. Kar, Prabhat Das, Renu Bala

DOI: 10.5958/0974-360X.2018.00024.0         Access: Open Access Read More

Author(s): A. Rajasekaran, R. Arivukkarasu, Joyce Mathew P, Raja Bonagiri, Ramadasu Partha Saradhi

DOI: Not Available         Access: Open Access Read More

Author(s): Ajay Raghuvanshi, D.M. Kar, Prabhat Das, Renu Bala

DOI: 10.5958/0974-360X.2017.00524.8         Access: Open Access Read More

Author(s): Jennifer Fernandes, Abhishek Kumar, Pankaj Kumar

DOI: Not Available         Access: Open Access Read More

Author(s): Geetha Rani. R, Banu. N

DOI: 10.5958/0974-360X.2017.00102.0         Access: Open Access Read More

Author(s): M. Krishnaveni, M. Kalaivani, C. Ragina Banu, G. Krishna Kumari

DOI: 10.5958/0974-360X.2015.00086.4         Access: Open Access Read More

Author(s): Golamari Siva Reddy, Kamma Srinivasulu, Botlagunta Mahendran, Ronda Srinivasa Reddy

DOI: 10.5958/0974-360X.2018.00748.5         Access: Open Access Read More

Author(s): Manoj M Nitalikar, Shrikant M Nitalikar, Girish A Gunjotikar and Prashant D Aragade

DOI: Not Available         Access: Open Access Read More

Author(s): Suman Kumari, Vinita Gupta, Sanchita Singh, Y.K. Gupta

DOI: 10.5958/0974-360X.2019.00031.3         Access: Open Access Read More

Author(s): Prafulla Sabale, Tinkal Gupta, Vidya Sabale

DOI: 10.5958/0974-360X.2018.00555.3         Access: Open Access Read More

Author(s): Usha Satyapal, Deepali Mahajan, Pratima Tatke, Vikram Naharwar

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags