Author(s): Dhania Novitasari, Riris Istighfari Jenie, Febri Wulandari, Rohmad Yudi Utomo, Dyaningtyas Dewi Pamungkas Putri, Jun-ya Kato, Edy Meiyanto


DOI: 10.52711/0974-360X.2021.00760   

Address: Dhania Novitasari1, Riris Istighfari Jenie1,2, Febri Wulandari1, Rohmad Yudi Utomo1,3, Dyaningtyas Dewi Pamungkas Putri1,4, Jun-ya Kato5, Edy Meiyanto1,2*
1Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada (UGM), Sekip Utara, Yogyakarta 55281, Indonesia.
2Macromolecular Engineering Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy UGM, Sekip Utara, Yogyakarta 55281, Indonesia.
3Medicinal Chemistry Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, UGM, Sekip Utara, Yogyakarta 55281, Indonesia.
4Pharmacology and Toxicology Laboratory, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, 55281, Indonesia.
5Laboratory of Tumor Cell Biology, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021

Triple-negative breast cancer (TNBC) remains as the deadliest cancer type due to the lack of treatment options. Hence, several attempts have been made to develop new anticancer for TNBC therapy. This study intended to challenge curcumin analog (CCA)-1.1, which is derived from pentagamavunone-1 structure, against the 4T1 cell line and TNBC cell model, covering the cytotoxic activity in correlation with cell cycle progression, apoptosis induction, reactive oxygen species (ROS) generation, and senescence evidence. The cell viability, cell cycle profile, apoptosis induction, intracellular ROS level, and senescence induction were determined in vitro using trypan blue exclusion, propidium iodide (PI) staining, Annexin-PI staining, dichlorofluorescein diacetate staining, and senescence-associated-ß-gal method. CCA-1.1 showed cytotoxic activity on 4T1 cells, giving half maximal inhibitory concentration value of 3?M, but was less toxic on non-cancerous 3T3-L1 cells. CCA-1.1 induced rapid cell death and inhibited cell cycle progression at the mitotic phase. Instead, of causing apoptosis, CCA-1.1 induced mitotic catastrophe. Furthermore, CCA-1.1 itself increased the intracellular ROS level and induced senescence, possibly through catastrophic cell death. Altogether, our preliminary study strengthens the potency of CCA-1.1 for its anticancer activities against TNBC cells and prospective to be pharmaceutically developed as a novel candidate for cancer therapy.

Cite this article:
Dhania Novitasari, Riris Istighfari Jenie, Febri Wulandari, Rohmad Yudi Utomo, Dyaningtyas Dewi Pamungkas Putri, Jun-ya Kato, Edy Meiyanto. Curcumin-like structure (CCA-1.1) induces permanent mitotic arrest (Senescence) on Triple-negative breast cancer (TNBC) cells, 4T1. Research Journal of Pharmacy and Technology. 2021; 14(8):4375-2. doi: 10.52711/0974-360X.2021.00760

Dhania Novitasari, Riris Istighfari Jenie, Febri Wulandari, Rohmad Yudi Utomo, Dyaningtyas Dewi Pamungkas Putri, Jun-ya Kato, Edy Meiyanto. Curcumin-like structure (CCA-1.1) induces permanent mitotic arrest (Senescence) on Triple-negative breast cancer (TNBC) cells, 4T1. Research Journal of Pharmacy and Technology. 2021; 14(8):4375-2. doi: 10.52711/0974-360X.2021.00760   Available on:

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