Author(s):
Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono
Email(s):
rizebudi.amalia@fk.unair.ac.id , agus.sulistyono@fk.unair.ac.id
DOI:
10.52711/0974-360X.2021.00759 
Address:
Rize Budi Amalia1*, Budi Prasetyo2, Ratna Dwi Jayanti1, Ivon Diah Wittiarika1, Widjiati3, Agus Sulistyono2
1Department of Midwifery, Faculty of Medicine University of Airlangga, Surabaya, Indonesia.
2Department of Obstetrics and Gynecology, Faculty of Medicine, University of Airlangga, Surabaya, Indonesia.
3Department of Anatomy, Faculty of Veterinary Medicine University of Airlangga, Surabaya, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 8,
Year - 2021
ABSTRACT:
Introduction: Stress during pregnancy affects physical and psychological change, resulting in wide range of mild to severe abnormalities including inflammation, abberant placentation, fetal growth defect, and the decrease quality of life in the fetus’ adulthood. Previous study showed that Folic Acid (FA) has the potential effect in the pregnant and fetus’ prosperities due to the protective properties in the embryogenesis. To that end, we created the stress mice model and treated with FA, assessment was obtained by the expression of IL-6 in placenta and birth weight observation to reflect the fetus quality. Methods: subjects were 21 pregnant mice (Mus musculus) that divided into three groups (n=7) comprised of normal pregnant mice, stress mice model, and stress mice model treated with FA groups. Stress induction started from gestational day (gd) 10 to gd-15 by chronic restrain stress and 379 lux bright light exposure each twice-a-day within 30 minutes to induce depressive and anxiety-like behaviours. Folic acid treatment was begun with the similar time with the stress induction by dose dependent 3 mg/kg bw/day once a day prior the stress induction. On gd-16, mice were euthanized and fetal weight was examined followed by placental tissue collection for immunochemistry staining. Results: Placental IL-6 expression were not statistically different in stressed pregnant-mice treated by folic acid (p=0.077). There was a significant difference in fetal weight (p= 0.0001). In addition, the expression of placental IL-6 was associated with fetal weight (p= 0.021). Conclusions: The FA treatment has the ability to increase the fetal birth weight but unable to decrease the IL-6 as the pro-inflammatory cytokine in stress-induced mice.
Cite this article:
Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono. Folic acid Supplementation on placental Interleukin-6 and offspring growth in prenatal stress mice model. Research Journal of Pharmacy and Technology. 2021; 14(8):4371-4. doi: 10.52711/0974-360X.2021.00759
Cite(Electronic):
Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono. Folic acid Supplementation on placental Interleukin-6 and offspring growth in prenatal stress mice model. Research Journal of Pharmacy and Technology. 2021; 14(8):4371-4. doi: 10.52711/0974-360X.2021.00759 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-65
REFERENCES:
1. ME C-R. Effects of prenatal stress on pregnancy and human development: mechanisms and pathways. Obstet Med. 2013;6(2):52-57.
2. Radhakrishnan SA. Psychological Wellbeing in Pregnancy. Asian J. Nur. Edu. and Research. 2012;2(4):202-208.
3. Madhavanprabhakaran GK DSM, Nairy KS. Prevalence of pregnancy anxiety and associated factors. International Journal of Africa Nursing Sciences. 2015;3(2015):1-7.
4. Palaparthi. Kaveri UJl. A Comparative Study to Assess the Anxiety Level of Hospitalized and Non- Hospitalized Antenatal women with High Risk Pregnancy at Selected Hospital, Guntur District, Andhra Pradesh. Asian J. Nursing Education and Research. 2019;9(4):493-497.
5. Fisher J dMM, Patel V. Prevalence and determinants of common perinatal mental disorders in women in low and lower-middle-income countries: a systematic review. Bulletin of WHO. Vol 902012.
6. Organization WH. Maternal mental health and child health and development in low and middle income countries: report of the meeting, Geneva, Switzerland, 30 January - 1 February, 2008.: World Health Organization;2008.
7. S) MLAK. Maternal Stress and Maternal and Foetal Outcome: A Comparative Study. Asian J. Nur. Edu. and Research. 2015;5(1):12-14.
8. Anilkumar B. Jarali SA. Depression during Pregnancy and Postpartum Period among Adolescent and Adult Mothers. Int. J. Nur. Edu. and Research. 2014;2(4):297-303.
9. Spiers JG CH, Sernia C, Lavidis NA. Front Neurosci. 2014; 8:456. Activation of the hypothalamic-pituitary-adrenal stress axis induces cellular oxidative stress. Front Neurosci. 2015;19(8):456.
10. Schieber M CNRRFiRSaOSCb-. Review: ROS Function in Redox Signaling and Oxidative Stress. Current biology. 2-14;24(10):453-426.
11. Swain D. Why doesn’t Mother Reject Fetus? The Immunological Concept of Pregnancy. Asian J. Nur. Edu. and Research. 2013;3(3):183-187.
12. Mittal M SM, Tran K, Reddy SP, Malik AB. Reactive Oxygen Species in Inflammation and Tissue Injury. Antioxid Redox Signal. 2014;20(7):1126-1167.
13. Garcia-Oscos F SH, Hall S, et al. The Stress-Induced Cytokine Interleukin-6 Decreases the Inhibition/Excitation Ratio in the Rat Temporal Cortex via Trans-Signaling. Biol Psychiatry. 2016;71(7):574-582.
14. Leff Gelman P, Mancilla-Herrera, I., Flores-Ramos, M. et al. The cytokine profile of women with severe anxiety and depression during pregnancy. BMC Psychiatry 2019;19(104).
15. Dahlgren J SA, Jansson T, Holmang A. Interleukin-6 in the Maternal Circulation Reaches the Rat Fetus in Mid-gestation. Pediatric research. 2006;60(2).
16. Barua S KS, Junaid MA. Folic acid supplementation in pregnancy and implications in health and disease. Journal of Biomedical science. 2014(77).
17. Krishna Kondragunta. V KV, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016;6(4):213-215.
18. Chitayat DC MD, Amitai Y, Kennedy D, Vohra S, Rieder M, Koren G. Folic acid supplementation for pregnant women and those planning pregnancy: 2015 update. J Clin Pharmacol. 2016;56(2):170-175.
19. Greenberg JA BS, Guan Y, Yu YH. Folic Acid supplementation and pregnancy: more than just neural tube defect prevention. Rev Obstet Gynecol. 2011;4(2):52-59.
20. Marques AH B-MA, Teixeira AL, Silverman MN. Maternal stress, nutrition and physical activity: Impact on immune function, CNS development and psychopathology. Brain Res. 2015;2015(1617):28-46.
21. Fekete K, Berti, C., Trovato, M. et al. Effect of folate intake on health outcomes in pregnancy: a systematic review and meta-analysis on birth weight, placental weight and length of gestation. Nutr J. 2012;11(75).
22. Grundwald NJ BP-. Prenatal stress programs neuroendocrine stress responses and affective behaviors in second generation rats in a sex-dependent manner. Psychoneuroendocrinology. 2015;62(2015):204-2016.
23. MEC R. Effects of prenatal stress on pregnancy and human development: mechanisms and pathways. Obstet Med. 2013;6(2):52-57.
24. Chen YY GM, Grattton R, Powell TL, Jansson T. Down-regulation of placental folate transporters in intrauterine growth restriction. J Nutr Biochem. 2018;59(2018):136-141.
25. Rondó P, Ferreira, R., Nogueira, F. et al. Maternal psychological stress and distress as predictors of low birth weight, prematurity and intrauterine growth retardation. Eur J Clin Nutr 2003;57(266-272).
26. Bhattacharjee A PS, Pal S, Maji B, Syamal AK, Mukherjee S. Synergistic protective effect of folic acid and vitamin B12 against nicotine-induced oxidative stress and apoptosis in pancreatic islets of the rat. Pharm Biol. 2016;54(3):433-444.