Author(s): Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono

Email(s): rizebudi.amalia@fk.unair.ac.id , agus.sulistyono@fk.unair.ac.id

DOI: 10.52711/0974-360X.2021.00759   

Address: Rize Budi Amalia1*, Budi Prasetyo2, Ratna Dwi Jayanti1, Ivon Diah Wittiarika1, Widjiati3, Agus Sulistyono2
1Department of Midwifery, Faculty of Medicine University of Airlangga, Surabaya, Indonesia.
2Department of Obstetrics and Gynecology, Faculty of Medicine, University of Airlangga, Surabaya, Indonesia.
3Department of Anatomy, Faculty of Veterinary Medicine University of Airlangga, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Introduction: Stress during pregnancy affects physical and psychological change, resulting in wide range of mild to severe abnormalities including inflammation, abberant placentation, fetal growth defect, and the decrease quality of life in the fetus’ adulthood. Previous study showed that Folic Acid (FA) has the potential effect in the pregnant and fetus’ prosperities due to the protective properties in the embryogenesis. To that end, we created the stress mice model and treated with FA, assessment was obtained by the expression of IL-6 in placenta and birth weight observation to reflect the fetus quality. Methods: subjects were 21 pregnant mice (Mus musculus) that divided into three groups (n=7) comprised of normal pregnant mice, stress mice model, and stress mice model treated with FA groups. Stress induction started from gestational day (gd) 10 to gd-15 by chronic restrain stress and 379 lux bright light exposure each twice-a-day within 30 minutes to induce depressive and anxiety-like behaviours. Folic acid treatment was begun with the similar time with the stress induction by dose dependent 3 mg/kg bw/day once a day prior the stress induction. On gd-16, mice were euthanized and fetal weight was examined followed by placental tissue collection for immunochemistry staining. Results: Placental IL-6 expression were not statistically different in stressed pregnant-mice treated by folic acid (p=0.077). There was a significant difference in fetal weight (p= 0.0001). In addition, the expression of placental IL-6 was associated with fetal weight (p= 0.021). Conclusions: The FA treatment has the ability to increase the fetal birth weight but unable to decrease the IL-6 as the pro-inflammatory cytokine in stress-induced mice.


Cite this article:
Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono. Folic acid Supplementation on placental Interleukin-6 and offspring growth in prenatal stress mice model. Research Journal of Pharmacy and Technology. 2021; 14(8):4371-4. doi: 10.52711/0974-360X.2021.00759

Cite(Electronic):
Rize Budi Amalia, Budi Prasetyo, Ratna Dwi Jayanti, Ivon Diah Wittiarika, Widjiati, Agus Sulistyono. Folic acid Supplementation on placental Interleukin-6 and offspring growth in prenatal stress mice model. Research Journal of Pharmacy and Technology. 2021; 14(8):4371-4. doi: 10.52711/0974-360X.2021.00759   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-65


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