Author(s): R. Sai Nandhini, R. Nirmala Nithya, K. Vidhya

Email(s): sainandhini@veltech.edu.in

DOI: 10.52711/0974-360X.2021.00756   

Address: R. Sai Nandhini*, R. Nirmala Nithya, K. Vidhya
Department of Biotechnology, Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
The present study investigates the presence of phytochemical compounds in the ethanol and methanol extract of Curculigo orchiodes, it was elucidated using Gas chromatography-Mass spectrometry. Qualitative phytochemical compounds of both extracts reveal the presence of flavonoids, tannins, phenol, protein, cardiac glycosides, terpenoids, and carbohydrate and have a higher level compounds in ethanol extracts of C.orchiodes. Fifteen bioactive phytocompounds were identified in the ethanol and methanol extract of C.orchiodes. GCMS reveal the presence of 2-Furanmethanol, Cyclopentanone, 2-Methyl, Formic acid, 2-Propenyl ester, 2,5-Furandione, 3-Methyl, phenol 3-Methoxy-, 2-Methoxy-4-vinyl phenol, 1,2-Benzenediol, 3-methoxy-, n-Hexadecanoic acid and phytol which has medicinal property such as antibacterial, antiviral, anticancer and antioxidant. C.orchiodes was assayed for antibacterial activity against the tested microorganisms such as Escherichia Coli, Klebsiella Pneumonia, Pseudomonas aeruginosa using well diffusion method. Ethanol extract show the highest zone of inhibition ranging from 3.4±0.200 to 5.28±0.350 compared with standard antibiotics. From this study, results highlight that the ethanol extract of C.orchiodes will be a valuable source for the production of herbal medicine and maintaining human health.


Cite this article:
R. Sai Nandhini, R. Nirmala Nithya, K. Vidhya. GC-MS analysis of Phytochemical compounds in different extracts of Curculigo orchiodes. Research Journal of Pharmacy and Technology. 2021; 14(8):4355-0. doi: 10.52711/0974-360X.2021.00756

Cite(Electronic):
R. Sai Nandhini, R. Nirmala Nithya, K. Vidhya. GC-MS analysis of Phytochemical compounds in different extracts of Curculigo orchiodes. Research Journal of Pharmacy and Technology. 2021; 14(8):4355-0. doi: 10.52711/0974-360X.2021.00756   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-62


REFERENCES:
1.    Narayana Aiyer MA and Kolammal M, Pharmacognosy of Ayurvedic Drugs. Trivandrum, Department of Pharmacognosy, University of Kerala.1963; 6–9.
2.    Yoganarasimhan SN. Medicinal Plants of India, Tamil Nadu, Bangalore, Cyber media.2000; 2: 169.
3.    Agrahari AK, Panda SK, Meher A and Padhan AR. Studies on the Anti-inflammatory properties of Curculigo orchioides Gaertn. Root tubers. Int. J. Pharm. Sci. and Res.2010; 1(8): 139-143.
4.    Wala BB and Jasrai YT. Micropropagation of an Endangered Medicinal Plant: Curculigo orchioides Gaertn, Plant Tissue Culture.2003; 13.
5.    Venukumar MR and Latha MS. Hepatoprotective effect of the methanolic extract of Curculigo orchioides in carbon tetrachloride treated male rats. Indian Journal of Pharmacology. 2002; 34: 269-275.
6.    Bafna AR and Mishra SH. Immunostimulatory effect of methanol extract of Curculigo orchioides on immunosuppressed mice. Journal of Ethnopharmacology. 2006; 104: 1-4.
7.    Raaman N, Selvarajan S, Gunasekaran CP, Ilango V, Mohammed Iiliyas and Balamurugan G. In vitro cytotoxic and anticancer activities of Curculigo orchioides Gaertn. Rhizomes. Journal of Pharmacy Research. .2009; 8: 1272-1273.
8.    Nagesh KS and Shanthamma C. Antibacterial activity of Curculigo orchioides rhizome extract on pathogenic bacteria. African Journal of Microbiology Research.2009; 1: 005-009.
9.    Jiao L, Peng cao D, Qin LP, Han T, Zhang QY, Zhu Z and yan F. Antiosteoporotic Activity of phenolic compounds from Curculigo orchioides. Phytomedicine.2009; 16: 874–881.
10.    Rao KS and Mishra SH. Antihepatotoxic principles from the rhizomes of Curculigo orchioides Gaertn. Indian Drugs.1997; 34: 68–71.
11.    Min X, Xiaohui Z, Zhaixiang D and Ming O. Effect of Yang tonifying herbs on myocardial b-adenoceptors of hypothyroid rabbits. J Ethnopharmacol.1998; 60: 43–51.
12.    Mital N Manvar. Antibacterial Activity of Leaves and Flowers of Ipomoea aquatica Forsk. (Convolvulacea), Asian Journal of Pharmaceutical Research. 2018; 8(2).
13.    Abhipsa V, Manasa M, Poornima G, Rekha C and Prashith Kekuda TR. In vitro Antibacterial Efficacy of Selected Plant Extracts, Streptomycin and their Combination. Asian J. Research Chem. 2018; 5(6).
14.    Arun P, Purushotham KG, Johnsy Jayarani, Vasantha Kumari and Chamundeeswari. Screening Antibacterial Activity of Various Extracts of Lawsonia inermis. Research J. Pharmacognosy and Phytochemistry. 2010; 2(3): 185-187.
15.    Salem Edrah, Eman Osela and Ashok Kumar. Preliminary phytochemical and antibacterial studies of Convolvulus arvensis and Thymus capitatus plants extracts. Research J. Pharmacognosy and Phytochemistry. 2013; 5(5): 220-223.
16.    Rao RVK, Ali N and Reddy MN. Occurrence of both sapogenin [yuccagenin] and alkaloid lycorine in Curculigo orchioides. Indian J. Pharm. Sci.1978; 3: 104-105.
17.    Garg SN, Mishra LN and Agarwal SK. Corchioside A- an orcinol glycoside from Curculigo orchioides. Phytochemistry.1989; 28: 1771-1772.
18.    Kubo M, Namba K, Nagamoto N, Nagao T, Nakanishi J, Uno H and Nishimura H. A new phenolic glucoside, curculigoside from rhizomes of Curculigo orchioides. Planta Med.1983; 1: 52-55.
19.    Nema RK and Ramawat KG. Isolation and identification of a new molecule from Curculigo orchioides (Hypoxidaceae). J. Chem. Pharm. Res. 2010; 2: 610-617.
20.    Tiwari RD and Misra G. Structural studies of the constituents of the rhizomes of Curculigo orchioides. Planta Med. 1976; 3: 291-294.
21.    Wu Q, Fu D X, Hou AJ, Lei GQ and Liu ZJ. Antioxidative phenols and phenolic glycosides from Curculigo orchioides. Chem Pharm Bull.2005; 53: 1065–1067.
22.    Kumari S, Sharma Y and Sharma N. New phytoconstituents from the rhizomes of Curculigo orchioides. Pharm Biol.2004; 42: 131–134.
23.    Gupta M, Achari B and Pal BC. Glucosides from Curculigo orchioides. Phytochemistry. 2005; 66: 659–663.
24.    Manore D, Pillai S, Joshi A and Punashiya R. Preliminary Phytochemical Screening and Antibacterial Activity of Ethyl Acetate Extract of Cuscuta reflexa Roxb. Research J. Pharm. and Tech. 2012; 5 (1).
25.    Saravana Kumar A, Avijit Mazumder, Vanitha J, Ganesh M, Venkateshwaran K, Saravanan VS and Sivakumar T. Antibacterial Activity of Methanolic Extract of Sesbania Grandiflora (Fabaceae). Research J. Pharm. and Tech. 2008; 1(1).
26.    Nirmala nithya R and Sai nandhini R. Screening and evaluation of secondary metabolites present in piper cubeba. AJPCR.2019; 12: 81-83.
27.    Sai nandhini R and Nirmala nithya R. Comparative studies on phytochemical screening of four different flowers – Couroupita guianensis, Bauhinia purpurea, Stenolobium stans, and Plumeria rubra. AJPCR. 2019; 11: 119-121
28.    Harsha Leena K, Kambhoja S and Vishesh Upadhyay. Evaluation of Antibacterial Activity of the Stem Bark of Alstonia scholaris Linn. R.Br. Research J. Pharmacognosy and Phytochemistry. 2011; 3(4): 180-183.
29.    Purushotham KG, Arun P, Johnsy Jayarani J, Vasanthakumari R and Chamundeeswari D. In Vitro Antibacterial and Antifungal Assay of Tectona grandis-A Screening Study. Research J. Pharmacognosy and Phytochemistry. 2010; 2(1): 57-60
30.    Arun Kumar, Ruchika Guleria and Anu Bhardwaj. Antibacterial Activity of Methanolic and Ethanolic Extracts of Leaves and Fruits of Ficus palmata Forssk. Research J. Pharmacognosy and Phytochemistry. 2012; 4(6): 310-313.
31.    Hameed IH, Hussein HJ, Kareem MA and Hamad NS. Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using gas chromatography-mass spectrometry (GC-MS), Journal of Pharmacognosy and Phytotherapy. 2015; 4: 107-125.
32.    Asif. A Review on Phytochemical and Ethnopharmacological Activities of Curculigo orchioides. Mahidol University Journal of Pharmaceutical Sciences. 2012; 39:1-10.
33.    Ruban P and Gajalakshmi K. In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens. Asian Pac J Trop Biomed. 2012; 5: 399-403.
34.    Mohan KR Konduri and Venkata Reddy Bogolu. Phytochemical Screening and In vitro Antibacterial activity of Some Medicinal Plants Belonging to Guntur, India. Research J. Pharm. and Tech. 2011; 7(11).
35.    Imad Hadi Hameed, Jassani, Mohammad J and Al Ghaidaa Jihadi mohammad. Anti-bacterial, Antifungal Activity and Chemical Analysis of Punica grantanum (Pomegranate peel) Using GC-MS and FTIR Spectroscopy. International Journal of Pharmacognosy and Phytochemical Research. 2016; 3: 480-494.
36.    Ruwanthi Premathilaka and Shanika Wiranjanee Silva. Bioactive compounds and antioxidant activity of Bunchosia armeniaca. World Journal of Pharmacy and Pharmaceutical Sciences. 2015; 10: 1237-1247.
37.    Ravikumar VR, Gopal V and Sudha T. Analysis of Phytochemical Constituents of Stem Bark Extracts of Zanthoxylum Tetraspermum Wight and Arn. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2012; 4: 391-402.
38.    Jeeva S and Krishnamoorthy AS. Antifungal Potential of Myco-molecules of Coprinopsis cinerea (Schaeff) S. Gray s.lat. against Fusarium spp. Madras Agric. J. 2018; 3: 56-60.
39.    Grace OM, Light ME, Lindsey KL, Moholland DA and Staden JV. Antibacterial activity and isolation of antibacterial compounds from fruit of the traditional African medicinal plant, Kigelia Africana. S Afr J Bot. 2012; 68: 220-222.
40.    Sharafzadeh S, Morteza Khosh-Khui and Javidnia K. Aroma profile of leaf and stem of lemon balm (Melissa officinalis L.) grown under greenhouse conditions. Adv Environ Biol. 2011; 5: 547-550.
41.    Parasuraman S, Raveendran R and Madhavrao C. GC-MS analysis of leaf extracts of Cleistanthus collinus Roxb. (Euphorbiaceae). Int J Ph Sci. 2009; 1: 284-286.
42.    Abdul Waheed, Muhammad Mansha Chohan, Dildar Ahmed and Nisar Ullah. The first report on the in vitro antimicrobial activities of extracts of leaves of Ehretia serrata. Saudi journal of biological sciences. 2018.
43.    Sridharan S, Meenaa V, Kavitha V and Nayagam AAJ. GC-MS study and phytochemical profiling of Mimosa pudica Linn. J Pharm Res. 2011; 4: 741-742.
44.    Inoue Y, Hada TA, Shiraishi K, Hirore H, Hamashima and Kobayashi S. Antimicrobial agents and Chemotherapy. 2005; 5: 1770-1774.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available