Author(s): Surekha. R, R. Ishwarya Kishore, Harini Srinivasamoorthy, V. Kishore Kumar, J. Athippathi

Email(s): editorjpsr@gmail.com

DOI: 10.52711/0974-360X.2021.00736   

Address: Surekha. R1, Dr. R. Ishwarya Kishore1*, Harini Srinivasamoorthy2, Dr. V. Kishore Kumar 3, Dr. J. Athippathi4
1Department of Physiology, ACS Medical College, Chennai 602105.
2Department of Microbiology, SRM Medical College Hospital and Research Centre, SRM IST, Kattankulathur, Chengalpet, 603211.
3MBBS, Saveetha Institute of Medical and Technical Science, Chennai 602105.
4B.S Biology, MD [Philipipines].
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Introduction: Chronic obstructive pulmonary disease (COPD) is a common disease characterized by persistent airflow obstruction with an enhanced chronic inflammatory response in the airways and the lung to noxious particles or gases. Oxygen deficiency (hypoxia) causes an increase in Reactive Oxygen Species (ROS). ROS can be neutralized with antioxidants, one of which is Bael leaf. Objective: To evaluate the antibacterial activity of plant leaf extract against the common isolated bacteria [Psuedomonas aeruginosa]. To know the Relaxant effect of bael leaf (Aegle marmelos) on oxidative stress in Sprague Dawley lung induced chronic systemic hypoxia. Methods: The study was conducted in SIMATS and Sri Akilandeswari Pharma College for the period of Six months from April 2020 to September 2020. 110 sputum samples were analysed for testing antibacterial activity. Neutralization of ROS has been determined by DPPH radical scavenging method. Contractile effect was done by the Organ bath method. Results: Among gram negative bacteria predominantly Pseudomonas aeruginosa is the commonest bacteria. The other important organisms are Klebsiella pneumoniae, E. coli, Acinetobacter species. Antibiotics showing high activity against Pseudomonas aeruginosa are Piperacillin tazobactam, Carbapenemase, and Quinolones. mucA gene mutation of P. aeruginosa could be used as predictors to identify poor prognosis in COPD patients. Analysis of antioxidant activity in Aegle marmelos shows 79.8% of reductions in DPPH free radicals for 100µg/ml. The minimum inhibitory concentration (MIC) value of the methanolic extract is around 256 µg/ml. 1mg/ml and 2mg/ml doses of the methanolic extract of this plant produced a positive relaxant effect in an isolated mouse bronchial rings, respectively. Conclusion: Methanolic extracts elicited the antagonistic effect against histamine and also relaxed the histamine-induced contractions, it can be concluded that relaxations induced by A. marmelos in mouse broncheal chain were due to the depression of H1-receptors. This study shows that Aegle marmelos can be effectively used in the treatment of COPD disorders.


Cite this article:
Surekha. R, R. Ishwarya Kishore, Harini Srinivasamoorthy, V. Kishore Kumar, J. Athippathi. Evaluation of Relaxant effect in Methanolic Extract of Aegle marmelos on Isolated Sprague Dawley Bronchial Tissue. Research Journal of Pharmacy and Technology. 2021; 14(8):4241-6. doi: 10.52711/0974-360X.2021.00736

Cite(Electronic):
Surekha. R, R. Ishwarya Kishore, Harini Srinivasamoorthy, V. Kishore Kumar, J. Athippathi. Evaluation of Relaxant effect in Methanolic Extract of Aegle marmelos on Isolated Sprague Dawley Bronchial Tissue. Research Journal of Pharmacy and Technology. 2021; 14(8):4241-6. doi: 10.52711/0974-360X.2021.00736   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-42


REFERENCES:
1.    Antonisen, N et al. Antibiotic therapy in exacerbations of chronic obstructive pulmonary disease. Ann. Intern. Med. 106: 196– 204.
2.    Aleemullah MF et al. Bacteriological Profile of Patients with AECOPD- Hospital Based Study. Int J Curr Microbiol App Sci. 2016; 5: p. 84-90.
3.    Bulpa P et al. Invasive pulmonary aspergillosis in patients withchronic obstructive pulmonary disease. Eur Respir J. 2007; 30: 782–800.
4.    Borthakur AK et al. Antibacterial Evaluation of Common Bacteriological Profile (Aerobic) in Acute Exacerbation of Chronic Obstructive Pulmonary Disease (AECOPD) in Tertiary Care Hospital (Silchar Medical College and Hospital). 2017; 6(3): 648-52
5.    Patel Mihir Kumar Maheshbhai, T. Sivabalan, Neesha Kiran Shinde. Effectiveness of Pranayama’s on Respiratory Health Status among Chronic Obstructive Pulmonary Disease (COPD) Patients Admitted in Pravara Rural Hospital, Loni (Bk). Asian J. Nur. Edu. and Research. 3(1): Jan.-March 2013; Page 33-36.
6.    Heera Jayasheela. Efficacy of Deep Breathing Exercise on dyspnoea among chronic obstructive pulmonary disease patients. Int. J. Nur. Edu. and Research. 2020; 8(1): 32-34.
7.    Heera Jayasheela. Awareness regarding Deep Breathing Exercise among the Chronic Obstructive Pulmonary Disease patients.
8.    Rahul Vikram Singh, Prabhakar Semwal, Taranjeet Kapoor. Medicinal Potential of Six Different Plant Species of Dehradun District, Uttarakhand. Asian J. Res. Pharm. Sci. 4(3): July-Sept. 2014; Page 134-139.
9.    Basu S et al. A epidemiological study of bacterial microbiology inacute exacerbation of chronic obstructive pulmonary diseasepatients in Kolkata, India. Asian journal of pharmaceutical and Clinical Research. 2013; 6: 112-1
10.    Chawla K et al. Bacteriological profile and their antibiogram fromcases of acute exacerbations of chronic obstructive pulmonarydisease: a hospital-based study. J Clinical Diagnostic Res. 2008; 2: 612-6.
11.    Manjula A.U., Prema Sampath Kumar. In vitro Evaluation of Biological Activity of Aegle marmelos (L.) Fruit. Research J. Pharm. and Tech. 2016; 9(4): 407-414. doi: 10.5958/0974-360X.2016.00075.5
12.    S. S. M. Umamageswari, B. Manipriya, M. Kalyani. Evaluation of Antibacterial activity of Zinc oxide Nanoparticles against Biofilm Producing Methicillin Resistant Staphylococcus aureus (MRSA). Research J. Pharm. and Tech. 2018; 11(5): 1884-1888. doi: 10.5958/0974-360X.2018.00350.5
13.    W. Wu, H. Badrane, S. Arora, H.V. Baker, S. Jin, MucA-mediated coordination of type III secretion and alginate synthesis in Pseudomonas aeruginosa, J. Bacteriol. 186 (22) (2004) 7575–7585.
14.    P. Maheshwari, I. Somasundaram. Health Related Quality of Life Measurement in Asthma and Chronic Obstructive Pulmonary Disease. Research J. Pharm. and Tech. 2016; 9(5): 518-520.
15.    Sivakumar G, Gopalasatheeskumar K, Gowtham K, Sindhu E, Akash Raj K, Rajaguru B, Sriram K, Kalaichelvan V K. Phytochemical analysis, Antioxidant and Antiarthritic activities of different solvent extract of Aegle marmelos L. unripe fruit. Research J. Pharm. and Tech. 2020; 13(6): 2759-2763.
16.    Karthika. U, Kaval Reddy Prasasvi, T. Diana Victoria, Didi Chinnu Raju. Antioxidant potential of aqueous extract of Aegle marmelos leaves. Research J. Pharm. and Tech. 2016; 9(4): 391-393.
17.    Bari MR, Hironet al. Microbes responsible for acute exacerbation of COPD. Mymensingh Med J. 2010; 19: 576–585
18.    Madhavi S et al Bacterial etiology of acute exacerbations ofchronic obstructive pulmonary disease. Journal of Microbiology and Biotechnology Research. 2012; 2(3): 440-44.
19.    Collee JG, Marmion BP, Fraser AG, Simmons A, editors. Mackieand Mc Cartney Practical Medical Microbiology. 14th ed. London:
20.    Clinical and Laboratory Standards Institute. 2011. Performance standards for antimicrobial susceptibility testing; Twenty first informational supplements. M100- S21. Wayne, PA: CLSI; 2011.
21.    Erkan, L. et al., 2008. Role of bacteria in acute exacerbations of chronic obstructive pulmonary disease, Int J Chron Obstruct Pulmon Dis. 3: 463 467.
22.    Gerard Rakesh et al. Bacterial agents causing acute exacerbations in Chronic Obstructive Pulmonary Disease (COPD) patients, their antibiograms to Extended Spectrum Beta- Lactamase (ESBL)production in a tertiary care hospital, India. Int J Curr Microbiol App Sci. 2013; 2(11): 273-82.
23.    Surat L, Chalita P, Cholpisut T, Somsak T, Suphara TI, Wanwasan N (Chemical constituents from Aegle marmelos. J Braz Chem. 2011; 22(13): 228-232
24.    Shihas Azeez, Sareena Kalathathoduvil, Shagoofa Basheer, Shafana Parveen Kankadyil, Dilip Chandrashekar. Use of inhaler device in patients with Asthma or Chronic obstructive pulmonary disease: Errors and Adherence problems. Research J. Pharm. and Tech. 2020; 13(9): 4130-4134.
25.    V. Arul, A. Miyazaki, R. Dhananjayan. Mechanism of the contractile effect of the alcoholic extract of Aegle marmelos corr. On isolated guinea pig ileum and tracheal chain. Phytomedicine. 11( 7): 679-68.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available