Author(s): Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao

Email(s): kvbhaskararao@vit.ac.in

DOI: 10.52711/0974-360X.2021.00735   

Address: Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao*
Marine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632014, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Protease constitutes the major group of catalytic enzymes which is involved in hydrolyzing peptide bond of proteins. Marine sediment sample were collected and protease producing bacterial isolates were identified by using casein as a substrate. The organisms were characterized by biochemical test and identified as Bacillus sp. In order to check for the production of protease enzyme, quantitative protease assay and Lowry’s method of protein estimation was carried out. The crude extract of protease was subjected for blood stain removal activity and the enzyme proved to be efficient which removed the stain in 15 min. The purpose of the current study is to isolate, identify, characterize and to carry out applications of protease enzyme from marine bacteria isolated from mangrove sediment samples.


Cite this article:
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao. Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria. Research Journal of Pharmacy and Technology. 2021; 14(8):4236-0. doi: 10.52711/0974-360X.2021.00735

Cite(Electronic):
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao. Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria. Research Journal of Pharmacy and Technology. 2021; 14(8):4236-0. doi: 10.52711/0974-360X.2021.00735   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-41


REFERENCES:
1.    Singh K, Bose H, Richa K, Karthik L, Gaurav K, Bhaskara Rao KV. Isolation and characterization of protease producing marine eubacteria. Journal of Agricultural Technology. 2012; 8(5): 1633-1649.
2.    Nurullah A, Fikret U. Production of extracellular alkaline proteases from Bacillus subtilis RSKK96 with solid state fermentation. Eurasian Journal of Bioscience, 2011; 5: 64-72.
3.    Sharma AK, Sharma V, Saxena J, Yadav B, Alam A, Prakash A. Optimization of protease production from bacteria isolated from soil. Applied Research Journal. 2015; 1(7): 388-394.
4.    Dalal R. Screening and Isolation of Protease producing bacteria from soil collected from different areas of Burhanpur region (MP) India. International Journal of Current Microbiology and Applied Sciences. 2015; 4(8): 597-606.
5.    Hanan SA. Isolation and Screening of Extracellular Proteases Produced by isolated Bacillus sp. Journal of Applied Pharmaceuticals Sciences. 2012; 2(9): 71-74.
6.    Ramanadevi V, Naik LS, Aruna K. Isolation and Biochemical characterization of protease isolated from Bacillus sp. SVN12. International journal of research in pure and applied microbiology. 2015; 3(3): 94-101.
7.    Richa K, Bose H, Singh K, Karthik L, Kumar G, Bhaskara Rao KV. Response surface optimization for the production of marine eubacterial protease and its application. Research Journal of Biotechnology. 2013; 8(4): 78-85.
8.    Galante YM, Formantici C. Enzyme Applications in detergency and in manufacturing industries. Current Organic chemistry. 2003; 7(13): 1399-1422.
9.    Lowry OH, Rosebrough NJ, Farr L, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951; 193: 265-275.
10.    Parekh S, Vinci VA, Strobel RJ. Improvement of microbial strains and fermentation processes. Applied Microbiology and Biotechnology. 2002; 54(3): 287-301.
11.    Ishikawa H, Ishimi K, Sugiura M, Sowa A, Fujiwara N. Kinetics and mechanism of enzymatic hydrolysis of gelatin layers of X-ray film and release of silver particles. Journal of Fermentation and Bioengineering. 1993; 76(4): 300-305.
12.    Anwar A, Saleemuddin M. Purification and characterization of a digestive alkaline protease from the larvae of Spilosoma obliqua. Archives of Insect Biochemistry and Physiology. 2002.
13.    Amoozegar MA, Malekadeh F, Malik KA. Production of amylase by newly isolated moderate halophile, Halobacillus sp. strain MA-2. Journal of Microbiol Methods. 2003; 52(3): 353-359.
14.    Govardhan CP, Margolin AP. Extremozymes for Industry. Chemistry and Industry. 1995; 17: 689-693.
15.    Sinha N, Satyanarayana T. Alkaline protease production by thermophilic Bacillus licheniformis. Indian Journal of Microbiology. 1991; 31: 425-430.
16.    Phadatare SU, Deshpande VV, Srinivasan MC. High activity alkaline protease from Conidiobolus coronatus (NCL 86.8.20): Enzyme production and compatibility with commercial detergents. Enzyme and Microbial Technology. 1993; 15: 72-76.
17.    Shivanand P, Jayaraman G. Production of extracellular protease from halotolerant bacterium, Bacillus aquimaris strain VITP4 isolated from Kumta coast. Process Biochemistry. 2009; 44: 1088-1094.
18.    Folin O, Ciocalteu V. On Tyrosine and Tryptophane Determinations in Proteins. Journal of Biological Chemistry. 1927; 73: 637-650
19.    Indarmawan T, Mustopa AZ, Budiarto BR, Tarman K. Antibacterial activity of extracellular protease isolated from and Algicolous fungus Xylaria psidii KT30 against Gram positive bacteria. HAYATI journal of Biosciences. 2016; 23: 73-78.
20.    Hmidet N, El-Hadj AN, Haddar A, Kanoun S, Alya SK, Nasri M. Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive. Biochemical Engineering Journal. 2009; 47(1-3): 71-79.

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