Author(s): Supiana Dian Nurtjahyani, Rasyadan Taufiq Probojati, Arif Nur Muhammad Ansori, Mochammad Amin, Retno Handajani

Email(s): diananin39@gmail.com

DOI: 10.52711/0974-360X.2021.00734   

Address: Supiana Dian Nurtjahyani1*, Rasyadan Taufiq Probojati2,3, Arif Nur Muhammad Ansori4, Mochammad Amin5, Retno Handajani6
1Faculty of Teaching and Education, Ronggolawe University, Tuban, 62381, East Java, Indonesia.
2Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, 61171, East Java, Indonesia.
3Faculty of Agriculture, Universitas Kadiri, Kediri, 64115, East Java, Indonesia.
4Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, East Java, Indonesia.
5Institute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, East Java, Indonesia.
6Faculty of Medicine, Universitas Airlangga, Surabaya, 60115, East Java, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
This study aimed to analyzed the haplotype network and constructed the phylogenetic tree topology of hepatitis C virus (HCV) in Tuban, Indonesia and those from other countries, to determine the visualize genealogical relationship and inference about gene connected. The HCV isolates were collected from blood transfusion center of Tuban, East Java, Indonesia in 2015 and as a comparison, also the sequences of HCV isolates were retrieved from the GenBank®, National Center of Biotechnology Information (NCBI), USA database. To constructed of distribution map was performed through median joining analysis using Haplotype Network v4.6, whereas to constructed and phylogenetic model analyses were constructed based on the NS5B and 5’UTR regions using MEGA X, maximum-likelihood method based on the Tamura-Nei model. In the HCV NS5B region haplotype network showed high haplotype diversity (Hd=1.00) in 22 haplotypes. Based on phylogenetic analysis 3 sample isolates Tuban (BDT-55-p23, BDT-79-p23 and BDT-112-p23) were identified one group and closely related to isolates from Indonesia. However, isolates Tuban also have the same sequence characters as the isolates from Thailand. It was also confirmed in haplotype network that the three isolates are connected to each other. The identification of HCV genotypes circulating in blood donors in the Tuban of East Java confirmed were closely related to HCV isolates from Indonesia and Thailand. Therefore, this result might contribute in a better medical management towards HCV.


Cite this article:
Supiana Dian Nurtjahyani, Rasyadan Taufiq Probojati, Arif Nur Muhammad Ansori, Mochammad Amin, Retno Handajani. Haplotype Network Analysis and Phylogenetic Tree Construction of Hepatitis C Virus (HCV) Isolated from Tuban, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(8):4231-5. doi: 10.52711/0974-360X.2021.00734

Cite(Electronic):
Supiana Dian Nurtjahyani, Rasyadan Taufiq Probojati, Arif Nur Muhammad Ansori, Mochammad Amin, Retno Handajani. Haplotype Network Analysis and Phylogenetic Tree Construction of Hepatitis C Virus (HCV) Isolated from Tuban, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(8):4231-5. doi: 10.52711/0974-360X.2021.00734   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-40


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