Author(s): Sudha N, Arambakkam Janardhanam Vanisree

Email(s): sudhaudhay2010@gmail.com

DOI: 10.52711/0974-360X.2021.00731   

Address: Sudha N*, Dr. Arambakkam Janardhanam Vanisree
Department of Biochemistry, University of Madras, Guindy Campus, Chennai - 600025, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Background: Nickel chloride, a widely occurring heavy metal compound is reported to be neuro toxic to organisms. It was shown to cause detrimental behaviour and biochemical changes. However, its effect on the nervous system of offspring of the victimized organisms and the combating strategies remain unexplained. Such studies could broaden our knowledge on the detrimental effect of toxic compounds and help in developing suitable combating strategies. Purpose: To evaluate the neuroprotective role of ethyl acetate extract (EA) of Curcuma amada in the brain of F1 progeny of Danio rerio (Zebrafish) which has been gestationally exposed to neurotoxicant nickel chloride. Methods: The group distribution of the study was Group I-Control Juvenile Fish, Group II-Juvenile fish obtained from female fishes subjected to nickel chloride exposure for 50 days, Group III-Juvenile fish obtained from female fish subjected to nickel chloride exposure and co- treated with EA extract of Curcuma amada (150µg) from 30th day to 50th day, Group-IV-Juvenile fish treated with EA extract of Curcuma amada (150µg) for 50 days. Neuroprotective role of ethyl acetate extract of Curcuma amada was evaluated in the juvenile fish (F1 progeny) by performing anxiety and memory test, visualizing ultra-structural changes of juvenile brain using transmission electron microscopy and biochemical assay of membrane bound enzymes. Result: In Colour preference test, there was marked reduction in anxiety behavior in the F1 progeny of exposed fish co treated with EA extract of Curcuma amada (Group III) compared to F1 progeny of metal exposed group (Group-II). In the novel object rejection test, there was a marked improvement in the memory and learning pattern of the EA extract of Curcuma amada treated fishes (Group III) compared to F1 progeny of metal exposed group (Group-II). TEM analysis showed clumped and condensed chromatin, peripheral margination and empty mitochondria with loss of cristae and lipid droplets in Nickel exposed model (Group-II) which was found to be alleviated in EA extract of Curcuma amada co-treated group (Group-III). Altered activities of Calcium ATPases and Sodium potassium ATPases was noted in Nickel chloride exposed Group (Group-II) which however were regulated by EA extract of Curcuma amada. Conclusion: The study suggests that Curcuma amada could combat the toxic effect of nickel chloride in the juvenile fish, the progeny of the exposed adult female.


Cite this article:
Sudha N, Arambakkam Janardhanam Vanisree. Neuroprotective role of Curcuma amada in F1 progeny of Juvenile Danio rerio (Zebrafish) from Nickel Chloride Induced Maternal Stress in Gestationally Challenged Adult Fishes. Research Journal of Pharmacy and Technology. 2021; 14(8):4215-0. doi: 10.52711/0974-360X.2021.00731

Cite(Electronic):
Sudha N, Arambakkam Janardhanam Vanisree. Neuroprotective role of Curcuma amada in F1 progeny of Juvenile Danio rerio (Zebrafish) from Nickel Chloride Induced Maternal Stress in Gestationally Challenged Adult Fishes. Research Journal of Pharmacy and Technology. 2021; 14(8):4215-0. doi: 10.52711/0974-360X.2021.00731   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-37


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