Author(s): Dharmendra B. Sharma, Parth Aphale, Vineet Sinnarkar, Sohan S. Chitlange, Asha Thomas

Email(s): parth.aphale@gmail.com

DOI: 10.52711/0974-360X.2021.00728   

Address: Dr. Dharmendra B. Sharma1, Dr. Parth Aphale2, Dr. Vineet Sinnarkar3, Prof. Dr. Sohan S. Chitlange4, Dr. (Mrs.) Asha Thomas5
1Principal, Professor and H.O.D., Department of Forensic Medicine and Toxicology, Dr. D.Y. Patil Homoeopathic Medical College and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India.
2Associate Professor and H.O.D, Department of Homoeopathic Pharmacy, Dr. D.Y. Patil Homoeopathic Medical College and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India.
3Associate Professor, Department of Forensic Medicine and Toxicology, Dr. D.Y. Patil Homoeopathic Medical College and Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India.
4Principal, Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research, Dr. D.Y. Patil Unitech Society, Pimpri, Pune, India.
5Professor and H.O.D, Department of Pharmaceutical Chemistry, Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research, Dr. D.Y. Patil Unitech Society, Pimpri, Pune, I

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Background: Chromatography is one of the important laboratory technique in which the components of a mixture are separated on an adsorbent in order to analyze, identify, purify and quantify a mixture. Thin Layer Chromatography (TLC)is used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound. High Performance Thin Layer Chromatography is a sophisticated and automated form of Thin Layer Chromatography (TLC). The procedure simultaneously processes the sample and standard that results in better analytical precision and accuracy at a faster pace. Pharmacological/ Toxicological action of Nux Vomica is because of its active principles present in the seeds namely strychnine, brucine etc. This research paper aims to corelate the active principles present in Nux Vomica with the toxicological action of the same. Materials and Methods: 1. Standard Nux Vomica mother tincture was tested for its alkaloid markers and its correlation with the toxicological action was studied. 2. Analysis of the mother tincture was done using High Performance Thin Layer Chromatography. 3. Stationary phase consisted of TLC Aluminium sheets with silica gel 60 F253 pre-coated layer (20cm x 10cm), thickness-0.2mm, no. of tracks-18, band length-6mm. 4. Mobile Phase consisted of Chloroform: Methanol (9.5:0.5). 5. The plate was developed in developing chamber and observed under U.V. Light. Results: Colours seen on the HPTLC Plates of samples are greenwhich corresponds to strychnine, dark blue which corresponds to brucine, orange to alkaloids fluorescent green to sterols and pink to fatty acids which are evident on the chromatogram. Conclusion: Therapeutic action of Nux Vomica as noted in Homoeopathic Materia Medica is because of the active principles like strychnine, brucine, alkaloids, sterols, fatty acids present in it which is evident from the chromatogram.


Cite this article:
Dharmendra B. Sharma, Parth Aphale, Vineet Sinnarkar, Sohan S. Chitlange, Asha Thomas. Standardization of Homoeopathic Mother Tincture of Strychnous nux vomica with the help of High-Performance Thin Layer Chromatography and correlation of its alkaloid markers with its Toxicological action. Research Journal of Pharmacy and Technology. 2021; 14(8):4203-6. doi: 10.52711/0974-360X.2021.00728

Cite(Electronic):
Dharmendra B. Sharma, Parth Aphale, Vineet Sinnarkar, Sohan S. Chitlange, Asha Thomas. Standardization of Homoeopathic Mother Tincture of Strychnous nux vomica with the help of High-Performance Thin Layer Chromatography and correlation of its alkaloid markers with its Toxicological action. Research Journal of Pharmacy and Technology. 2021; 14(8):4203-6. doi: 10.52711/0974-360X.2021.00728   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-34


REFERENCES:
1.    Mandal P. and Mandal B., A Textbook of Homoeopathic Pharmacy, thoroughly revised and enlarged 3rd Edition 2012, Page. 95-103: 346
2.    Dharmendra. B. Sharma et.al. Qualitative Analysis of Calendula officinalis Homeopathic Mother Tincture with the help of High Performance Thin Layer Chromatography. Research J. Pharm. and Tech 2020; 13(3): 1113-1116.
3.    Abid Kamal et.al. Simultaneous HPTLC determination of strychnine and brucine in Strychnous nux vomica seed, J Pharm Bioallied Sci, 2012; 4(2): 134-139
4.    Bardale R, Principles of Forensic Medicine and Toxicology, 1st Edition 2011, J.P. Publication, Page-535-537
5.    Boricke W, New Manual of Homoeopathic Materia Medica and Repertory, Augmented edition based on 9th edition, page 463, 464

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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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