Author(s): Mefina Kuntjoro, Bambang Agustono, Eric Priyo Prasetyo, Sherman Salim, Fedik Abdul Rantam, Nike Hendrijantini

Email(s): nike-h@fkg.unair.ac.id

DOI: 10.52711/0974-360X.2021.00696   

Address: Mefina Kuntjoro1,2, Bambang Agustono2, Eric Priyo Prasetyo3, Sherman Salim2, Fedik Abdul Rantam4,5, Nike Hendrijantini2*
1Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Laboratory of Virology and Immunology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
5Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
Background: Diabetes Mellitus is a systemic disease characterized by an increase in blood glucose which, in the long term, enhances advanced glycation end product and leads to impaired osteogenesis. In prosthodontics, the osteogenic process plays an important role in successful treatment. Purpose: The purpose of this study is to determine the effect of Advanced Glycation End products (AGEs) present in Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) on osteogenesis and calcification. Materials and Methods: MSCs isolated from human umbilical cord were cultured and underwent expansion up to passage 5. The research sample was divided into two sub-groups; a treatment group (osteogenic medium+AGE-BSA medium) and a control group (osteogenic medium) each of which underwent three replications. Samples were examined immunocytochemically on days 1, 3, 7, 8, 9, 12, 14 and 21 to quantify the level of RUNX2 expression. Alizarin red staining was performed on day 21. Results: In the treatment group, RUNX2 expression increased on day 3, reaching a peak on days 7 and 14. That expression decreased on day 8. In the control group, the expression of RUNX2 reached its peak on day 8 before decreasing on day 9. The presence of alizarin red indicated calcification in the control medium, but less mineralization in the treatment group. Conclusion: The research indicated that AGE-BSA enhances the production of RUNX2 expression in hUCMSCs at both the initial and maturation stages. This finding was supported by the results of alizarin red staining which indicated that increased levels of RUNX2 produced less mineralization.


Cite this article:
Mefina Kuntjoro, Bambang Agustono, Eric Priyo Prasetyo, Sherman Salim, Fedik Abdul Rantam, Nike Hendrijantini. The Effect of Advanced Glycation End Products (AGEs) on Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with regard to Osteogenesis and Calcification. Research Journal of Pharmacy and Technology. 2021; 14(8):4019-4. doi: 10.52711/0974-360X.2021.00696

Cite(Electronic):
Mefina Kuntjoro, Bambang Agustono, Eric Priyo Prasetyo, Sherman Salim, Fedik Abdul Rantam, Nike Hendrijantini. The Effect of Advanced Glycation End Products (AGEs) on Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with regard to Osteogenesis and Calcification. Research Journal of Pharmacy and Technology. 2021; 14(8):4019-4. doi: 10.52711/0974-360X.2021.00696   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-2


REFERENCES:
1.    Panchal I, Panigrahi B, Patel C. Recent Advancement towards Treatment of Diabetes. Res J Pharmacol Pharmacodyn [Internet]. 2010 [cited 2020 Jun 2]; 2(1): 12–22. Available from: http://rjppd.org/HTMLPaper.aspx?Journal=Research Journal of Pharmacology and Pharmacodynamics; PID=2010-2-1-24
2.    Bhanushali S, Modh K, Anand I, Patel C, Dave J. Novel Approaches for Diabetes Mellitus: A Review. Res J Pharmacol Pharmacodyn [Internet]. 2010 [cited 2020 Jun 2];2(2):141–7. Available from: http://rjppd.org/HTMLPaper.aspx?Journal=Research Journal of Pharmacology and Pharmacodynamics;PID=2010-2-2-29
3.    Skorecki K, Chertow GM, Marsden PA, Taal MW, Yu AS. Brenner and Rector’s The Kidney. 10th ed. Elsevier; 2016. 2463 p.
4.    Carneiro VL, Fraiz FC, Ferreira F de M, Pintarelli TP, Oliveira ACB, Boguszewski MC da S. The influence of glycemic control on the oral health of children and adolescents with diabetes mellitus type 1. Arch Endocrinol Metab. 2015; 59(6): 535–40.
5.    Panari H, Vegunarani M. Study on Complications of Diabetes Mellitus among the Diabetic Patients. Asian J Nurs Educ Res. 2016 Jun 28; 6(2): 182.
6.    Gaviraj EN, Ramarao A, Veeresham C, Shivakumar B, Kalyane N V., Biradar SM. Inhibitory activities of some folklore remedies on Aldose reductase of rat lens and generation of advanced glycation end products. Res J Pharm Technol. 2019 Apr 1; 12(4): 1947–52.
7.    Dhaon P, Shah VN. Type 1 diabetes and osteoporosis: A review of literature [Internet]. Vol. 18, Indian Journal of Endocrinology and Metabolism. Medknow Publications; 2014 [cited 2020 Mar 29]. p. 159–65. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24741510
8.    Parithimarkalaignan S, Padmanabhan T V. Osseointegration: An update. Vol. 13, Journal of Indian Prosthodontist Society. Wolters Kluwer -- Medknow Publications; 2013. p. 2–6.
9.    Hendrijantini N. Mekanisme regenerasi tulang mandibula osteoporosis dengan pemberian human umbilical cord mesenchymal stem cells. Universitas Airlangga; 2015.
10.    Diksha, Patyar S. Role of stem cells in treatment of different diseases. Vol. 11, Research Journal of Pharmacy and Technology. Research Journal of Pharmacy and Technology; 2018. p. 3667–78.
11.    Kumari R. Stem Cell. Int J Nurs Educ Res. 2018; 6(4): 443–6.
12.    Tiwari RK, Sharma V, Pandey R, Shukla SS. Stem cells: basics and its prospective uses in medical field. Res J Pharm Technol. 2018; 11(4): 1530–4.
13.    Wang L, Ott L, Seshareddy K, Weiss ML, Detamore MS. Musculoskeletal tissue engineering with human umbilical cord mesenchymal stromal cells. Vol. 6, Regenerative Medicine. 2011. p. 95–109.
14.    Selvi ST. Stem Cell Therapy. Int J Adv Nurs Manag. 2017; 5(4): 361–4.
15.    Azeem S, Raj S, Kajal K, Thiagarajan P. Umbilical cord stem cells: A review. Res J Pharm Technol. 2018 Jun 1; 11(6): 2709–14.
16.    Lu YQ, Lu Y, Li HJ, Cheng XB. Effect of advanced glycosylation end products (AGEs) on proliferation of human bone marrow mesenchymal stem cells (MSCs) in vitro. Vitr Cell Dev Biol - Anim. 2012; 48(9): 599–602.
17.    Lavrentieva A, Majore I, Kasper C, Hass R. Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. Cell Commun Signal. 2010; 8.
18.    Ejtehadifar M, Shamsasenjan K, Movassaghpour A, Akbarzadehlaleh P, Dehdilani N, Abbasi P, et al. The effect of hypoxia on mesenchymal stem cell biology [Internet]. Vol. 5, Advanced Pharmaceutical Bulletin. Tabriz University of Medical Sciences; 2015 [cited 2020 Mar 29]. p. 141–9. Available from: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L604742818
19.    Yamagishi S. Role of Advanced Glycation End Products (AGEs) in Osteoporosis in Diabetes. Curr Drug Targets [Internet]. 2011 Dec 26 [cited 2020 Mar 29]; 12(14):2096–102. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22023404
20.    Arai M, Shibata Y, Pugdee K, Abiko Y, Ogata Y. Effects of reactive oxygen species (ROS) on antioxidant system and osteoblastic differentiation in MC3T3-E1 cells. IUBMB Life. 2007;59(1):27–33.
21.    Hendrijantini N, Kresnoadi U, Salim S, Agustono B, Retnowati E, Syahrial I, et al. Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with Gelatin Solvent. J Biomed Sci Eng [Internet]. 2015 [cited 2018 Jan 1]; 08(07): 420–8. Available from: http://www.scirp.org/journal/doi.aspx?DOI=10.4236/jbise.2015.87039
22.    Kanase K, Shinde N, Bharti D, Undale V, Bhosale A. Diabetes Macroangiopathy- A Review. Res J Pharmacol Pharmacodyn [Internet]. 2009 [cited 2020 Jun 3];1(2):45–9. Available from: http://rjppd.org/AbstractView.aspx?PID=2009-1-2-13
23.    Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative Analysis of Mesenchymal Stem Cells from Bone. Stem Cells. 2006;1294–301.
24.    Ramakrishna V, Janardhan P, Sudarsanareddy L. Stem cells and regenerative medicine - a review. Annu Rev Res Biol. 2011;1(4):79–110.
25.    Dominici M, Blanc K Le, Mueller I, Marini FC, Krause DS, Deans RJ, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315–7.
26.    Komori T. Runx2, a multifunctional transcription factor in skeletal development [Internet]. Vol. 87, Journal of Cellular Biochemistry. 2002 [cited 2020 Feb 11]. p. 1–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12210716
27.    Hu L, Yin C, Zhao F, Ali A, Ma J, Qian A. Mesenchymal stem cells: Cell fate decision to osteoblast or adipocyte and application in osteoporosis treatment. Vol. 19, International Journal of Molecular Sciences. MDPI AG; 2018.
28.    Notsu M, Yamaguchi T, Okazaki K, Tanaka K, Ogawa N, Kanazawa I, et al. Advanced Glycation End Product 3 (AGE3) Suppresses the Mineralization of Mouse Stromal ST2 Cells and Human Mesenchymal Stem Cells by Increasing TGF- ␤ Expression and Secretion. 2014; 3(July): 2402–10.
29.    Kanazawa I. Association of the roles of advanced glycation end products and osteocalcin between bone metabolism and vascular failure. Vasc Fail [Internet]. 2017 Apr 10 [cited 2020 Mar 29]; 1(1): 30–8. Available from: https://www.jstage.jst.go.jp/article/vascfail/1/1/1_30/_article
30.    Sudiana IK. Hantaran sinyal pada proses inflamasi. Surabaya: Airlangga University Press; 2017. 14–15 p.
31.    Gregory CA, Gunn WG, Peister A, Prockop DJ. An Alizarin red-based assay of mineralization by adherent cells in culture: Comparison with cetylpyridinium chloride extraction. Anal Biochem. 2004 Jun 1; 329(1): 77–84.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available