Anna Buzlama, Solaiman Doba, Sali Daghir, Karpova Evgenija Leonidovna, Ghadeer Balloul
Anna Buzlama1, Solaiman Doba1*, Sali Daghir2, Karpova Evgenija Leonidovna1, Ghadeer Balloul3
1 Department of Pharmacology and Clinical Pharmacology, Faculty of Pharmacy, Voronezh State University, Russia.
2Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Tishreen University, Latakia, Syria.
3Department of Pharmaceutical Technology, Faculty of Pharmacy, Belgorod State National Research University, Russia.
Volume - 14,
Issue - 8,
Year - 2021
In this study, the antiulcer activity of a gel based on high-viscosity chitosan was studied in models of NSAID and ethanol-induced ulcerogenesis. To simulate damage to the gastric mucosa in the NSAID model, diclofenac sodium was administered to experimental animals at a dose of 50mg/kg. An antiseptic intestinal and astringent agent was chosen as reference drug: bismuth tripotassium dicitrate at a dose of 0.017g/kg. The studied gel was used in 3 doses (0.08, 0.16 and 0.24ml/100g of body weight). In ethanol model, ulcers were induced by a single administration of ethanol 96% at a dose of 5ml/kg. Omeprazole at a dose of 20mg/kg was used as reference drug in this model. Chitosan-based gel was administrated in this model at a dose of 0.16ml per 100g of animals, which corresponds to the minimum available dose with antiulcer activity in the NSAID model. All investigated substances were injected intragastrically using a gastric tube. As a result of this research, it was found that the chitosan-based gel is effective in the NSAID gastropathy model but not effective in the ethanol model. In the NSAID gastropathy model, after a single oral administration of chitosan-based gel at doses of 0.16 and 0.24ml/ 100g, sufficient antiulcer activity was revealed, which was 2.4 and 4.694, respectively, and exceeded the effect of the reference drug, bismuth tripotassium dicitrate. In the ethanol model, the results of experimental studies showed that the reference drug, omeprazole, provides antiulcer activity with a calculated value of the antiulcer activity index of 2.18. After the administration of a chitosan-based gel at a dose of 0.16 ml per 100g of body weight of animals, compared with the control, the calculated value of antiulcer activity was 1.18, which characterizes the absence of an antiulcer effect.
Cite this article:
Anna Buzlama, Solaiman Doba, Sali Daghir, Karpova Evgenija Leonidovna, Ghadeer Balloul. Study of Antiulcer activity of a hydrogel based on chitosan. Research Journal of Pharmacy and Technology. 2021; 14(8):4101-6. doi: 10.52711/0974-360X.2021.00710
Anna Buzlama, Solaiman Doba, Sali Daghir, Karpova Evgenija Leonidovna, Ghadeer Balloul. Study of Antiulcer activity of a hydrogel based on chitosan. Research Journal of Pharmacy and Technology. 2021; 14(8):4101-6. doi: 10.52711/0974-360X.2021.00710 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-16
1. Anurova MN, Bakhrushina EO, Diomina NB. Design of the composition and technology of long-acting oral nimesulide gel Pharmacy. 2016; 6: 30-34. (in Russ.)
2. Satyanarayana DA, Kulkarni PK, Shivakumar HG. Gels and jellies as a dosage form for dysphagia patients: A Review. Current Drug Therapy. 2011; 6: 79-86.
3. Zagorulko EY, teslev AA. Gels for oral administration. Part 1. Medical products and dietary supplements (review). Drug development & registration. 2017; (3): 42-48. (in Russ.)
4. Arai K, Kinumaki T, Fujita T. Toxicity of chitosan. Bulletin Of Tokai Regional Fisheries Research Laboratory. 1968; 56: 89-94.
5. Scriabin KG, Vikhoreva GA, Varlamov VP. Chitin and chitosan: obtaining, properties and application. [Chitin and Chitosan: Preparation, Properties and Applications]. Moscow Science. Russia. 2002. (in Russ.)
6. Nemtsev SV. Complex technology of chitin and chitosan from the shell of crustaceans. [Integrated technology of chitin and chitosan from crustacean shells]. Publishing house VNIRO. Russia. 2006. (in Russ.)
7. Kumar MNVR. A review of chitin and chitosan applications. Reactive and Functional Polymers. 2000; 46 (1): 1-27.
8. Sionkowska A, et al. Preparation and characterization of collagen / chitosan / hyaluronic acid thin films for application in hair care cosmetics. Pure and Applied Chemistry. 2017; 89 (12): 1829-1839.
9. Gomaa YA, et al. Chitosan microparticles incorporating a hydrophilic sunscreen agent. Carbohydrate Polymers. 2010; 81: 234-242.
10. Dodane V and Vilivalam VD. Pharmaceutical applications of chitosan. Pharmaceutical Science & Technology Today. 1998; 1 (6): 246-253.
11. Mohammedi Z. Chitosan and Chitosan Oligosaccharides: Applications in Medicine, Agriculture and Biotechnology. International Journal of Bioorganic Chemistry. 2017; 2 (3): 102-106.
12. Onsoyen E and Skaugrud O. Metal recovery using chitosan. J. Chem. Technol. and Biotechnol. 1990; 49 (4): 395-404.
13. Buzlama A, Doba S, et al. Pharmacological and biological effects of chitosan. Research Journal of Pharmacy and Technology. 2020; 13 (2): 1043-1049.
14. Fedosov PA, et al. Preclinical study of the efficacy and safety of wound healing gel containing chitosan, taurine and allantoin. Research Result. 2017; 3 (2): 14-28.
15. Xie W. et al. Antioxidant activity of water-soluble chitosan derivatives. Bioorganic & Medicinal Chemistry Letters. 2001; 11 (13): 1699-1701.
16. Pogorielov MV and Sikora VZ. Chitosan as a hemostatic agent: current state. European Journal of Medicine. Series B. 2015; 2 (1): 24-33.
17. Friedman AJ, at al. Antimicrobial and anti-inflammatory activity of chitosan-alginate nanoparticles: a targeted therapy for cutaneous pathogens. The Journal of Investigative Dermatology. 2013; 133: 1231-1239.
18. Alsarra IA. Chitosan Topical Gel Formulation in the Management of Burn Wounds. International Journal of Biological Macromolecules. 2009; 45: 16.
19. Ozcelik E, et al. Protective effect of chitosan treatment against acetaminophen-induced hepatotoxicity. The Kaohsiung Journal of Medical Sciences. 2014: 30: 286-290.
20. Xie Y, et al. The study of antibacterial effect of chitosans and their derivatives on Helicobacter pylori in vitro and influence factor. National Digital Science Library November. 2005; 21 (11): 1343 – 1347
21. Zaki NI, Hassanin LA, Khattab AAM. Evaluation of Newly Formulated Antiulcer Drug on Experimental Ulcer Model. J. Drug Res. Egypt. 2014; 35(1): 81-92.
22. Ito M, Ban A, Ishihara M. Anti-ulcer effects of chitin and chitosan, healthy foods, in rats. Jpn J Pharmacol. 2000; 82(3): 218-25.
23. Buzlama AV, et al. Experimental pharmacology - principles, models, analysis: monograph. Voronezh State University Publishing and Printing Center, Voronezh. 2013.
24. Hajrezaie M et al. Biochanin A Gastroprotective Effects in Ethanol-Induced Gastric Mucosal Ulceration in Rats. PLOS One. 2015; 10(3): e0121529.
25. Slivkin AI, Chernov YN, Buzlama AV. Pallet for planimetric measuring of objects in biology and medicine. RF Utility Model. Patent № 114147. publ. 2012. (in Russ.)
26. Buzlama AV, et al. Preclinical studies of medicinal substances: a tutorial. GEOTAR-Media, Moscow. 2017. (in Russ.)
27. Elizarova ON. A manual on toxicology for laboratory assistants. Medicine, Moscow. 1974. (in Russ.)
28. Suleimanov SM, et al. Methods of morphological research: method. allowance. Voronezh State Agricultural University, Voronezh. 2012. (in Russ.)
29. Sepetliev DA. Statistical Methods in Scientific Medical Research. Medicine, Moscow. 1968. (in Russ.)