Author(s): Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein

Email(s): laylataha.23@yahoo.com

DOI: 10.52711/0974-360X.2021.00706   

Address: Layla T. Yassen1, Saad L. Hamed2, Ban O. Abdulsattar3, Asmaa A. Hussein4
1,2,3Mustansiriyah University, College of Science, Department of Biology, Baghdad, Iraq.
4Al-Nahrain University, College of Science, Department of Biotechnology, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 14,      Issue - 8,     Year - 2021


ABSTRACT:
The aim of this study was purification and characterization of CTX-M-15 as a medically important enzyme from locally Klebsiella pneumoniae isolate, CTX-M-15 enzyme was subjected to two purification steps including: precipitation with 80% ammonium sulfate saturation and gel filtration chromatography by using Sepharose -6B column. Specific activity of purified enzyme has been increment up to 21.9 IU/mg with 7.3 purification folds and 69% enzyme recapture. Characterization study of purified enzyme demonstrated that the M.wt. of CTX-M-15 produced by K. pneumoniae was almost 32.2 kDa. The maximal enzyme activity at (pH 7.0), and enzyme settled at pH 6-7. The enzyme also revealed a full activity at a range of temperature between 30-37oC. Enzyme activity has inhibited powerfully in the existence of EDTA and calcium chloride, when added separately at a constant concentration. Moreover, copper chloride, and ferric chloride also caused a strong inhibition to the enzyme activity while cloxacillin showed a minor effect on enzyme activity.


Cite this article:
Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein. Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16. Research Journal of Pharmacy and Technology. 2021; 14(8):4080-4. doi: 10.52711/0974-360X.2021.00706

Cite(Electronic):
Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein. Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16. Research Journal of Pharmacy and Technology. 2021; 14(8):4080-4. doi: 10.52711/0974-360X.2021.00706   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-12


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