Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein
Layla T. Yassen1, Saad L. Hamed2, Ban O. Abdulsattar3, Asmaa A. Hussein4
1,2,3Mustansiriyah University, College of Science, Department of Biology, Baghdad, Iraq.
4Al-Nahrain University, College of Science, Department of Biotechnology, Baghdad, Iraq.
Volume - 14,
Issue - 8,
Year - 2021
The aim of this study was purification and characterization of CTX-M-15 as a medically important enzyme from locally Klebsiella pneumoniae isolate, CTX-M-15 enzyme was subjected to two purification steps including: precipitation with 80% ammonium sulfate saturation and gel filtration chromatography by using Sepharose -6B column. Specific activity of purified enzyme has been increment up to 21.9 IU/mg with 7.3 purification folds and 69% enzyme recapture. Characterization study of purified enzyme demonstrated that the M.wt. of CTX-M-15 produced by K. pneumoniae was almost 32.2 kDa. The maximal enzyme activity at (pH 7.0), and enzyme settled at pH 6-7. The enzyme also revealed a full activity at a range of temperature between 30-37oC. Enzyme activity has inhibited powerfully in the existence of EDTA and calcium chloride, when added separately at a constant concentration. Moreover, copper chloride, and ferric chloride also caused a strong inhibition to the enzyme activity while cloxacillin showed a minor effect on enzyme activity.
Cite this article:
Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein. Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16. Research Journal of Pharmacy and Technology. 2021; 14(8):4080-4. doi: 10.52711/0974-360X.2021.00706
Layla T. Yassen, Saad L. Hamed, Ban O. Abdulsattar, Asmaa A. Hussein. Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16. Research Journal of Pharmacy and Technology. 2021; 14(8):4080-4. doi: 10.52711/0974-360X.2021.00706 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-12
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