Moath Alqaraleh, Violet Kasabri, Ibrahim Al-Majali, Ahmad Aljaafreh, Nihad Al-Othman, K. Khleifat, Nafe M Al‐Tawarah, Haitham Qaralleh, Alia S. Khwaldeh, Sundus Alalawi, Mohammad al majali
Moath Alqaraleh1*, Violet Kasabri2, Ibrahim Al-Majali3, Ahmad Aljaafreh3, Nihad Al-Othman4, K. Khleifat5, Nafe M Al‐Tawarah3, Haitham Qaralleh3, Alia S. Khwaldeh6, Sundus Alalawi2, Mohammad al majali7
1Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan.
2Department of Pharmacy, Faculty of Pharmacy. The University of Jordan, Amman, Jordan.
3Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan.
4Division of Anatomy, Biochemistry, and Genetics. Faculty of Medicine and Health Sciences, An-Najah National University, Nablus.
5Department of Biology, Mutah University, Karak, Mutah, 61710 Jordan.
6Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid, Jordan.
7Department of Biological Sciences, Faculty of Science. The University of Jordan, Amman, Jordan.
Volume - 14,
Issue - 7,
Year - 2021
Background and aims: Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and Methods: the in vitro antioxidant activity of BCAAs was assessed using free radical 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays. For further check, a qRT-PCR technique was madefor detection the extent of alterations in gene expression of antioxidative enzymes (catalase and glutathione peroxidase (Gpx)) in lipopolysaccharides (LPS(-induced macrophages RAW 264.7 cell line. Additionally, BCAAs antioxidant activity was evaluated based on plasma H2O2 levels and xanthine oxidase (XO) activity in prooxidative LPS-treated mice. Results: Different concentrations of BCAAs affected on DPPH radical scavenging activity but to lesser extent than the ascorbic acid. Besides, BCAAs obviously upregulated the gene expression levels of catalases and Gpx in LPS-modulated macrophage RAW 264.7 cell line. In vivo BCAAs significantly minimized the level of plasma H2O2 as well as the activity of XO activity under oxidative stress. Conclusion: our current findings suggest that BCAAs supplementation may potentially serve as a therapeutic target for treatment of oxidative stress occurs with atherosclerosis, IR-diabetes, MetS and tumorigenesis.
Cite this article:
Moath Alqaraleh, Violet Kasabri, Ibrahim Al-Majali, Ahmad Aljaafreh, Nihad Al-Othman, K. Khleifat, Nafe M Al‐Tawarah, Haitham Qaralleh, Alia S. Khwaldeh, Sundus Alalawi, Mohammad al majali. Branched chain amino Acids as in vitro and in vivo Anti-Oxidation Compounds. Research Journal of Pharmacy and Technology. 2021; 14(7):3899-4. doi: 10.52711/0974-360X.2021.00677
Moath Alqaraleh, Violet Kasabri, Ibrahim Al-Majali, Ahmad Aljaafreh, Nihad Al-Othman, K. Khleifat, Nafe M Al‐Tawarah, Haitham Qaralleh, Alia S. Khwaldeh, Sundus Alalawi, Mohammad al majali. Branched chain amino Acids as in vitro and in vivo Anti-Oxidation Compounds. Research Journal of Pharmacy and Technology. 2021; 14(7):3899-4. doi: 10.52711/0974-360X.2021.00677 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-74
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