Author(s): Ashutosh Pal Jain, Gajendra Pratap Choudhary


DOI: 10.52711/0974-360X.2021.00662   

Address: Ashutosh Pal Jain1*, Gajendra Pratap Choudhary2
1Bhagyoday Tirth Pharmacy College, Sagar, Madhya Pradesh 470001, India.
2School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore, Madhya Pradesh 452001, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021

Background: The purpose of this study was to evaluate the antioxidant potential of Sphaeranthus indicus and Abrus precatorius leaves. Methods: Both plant leaves powder was exhaustively extracted by soxhlet apparatus with various solvents of increasing order of polarity, petroleum ether followed by chloroform, methanol and water. The total phenol, tannins and flavonoids content were determined spectrophotometrically. Quantitative estimation of phenolic and flavonoid compounds in the extracts was estimated by using the Folin–Ciocalteu reagent and aluminum chloride colorimetric method respectively. The antioxidant potential of all these extracts was evaluated by reducing ability assay and DPPH radical scavenging assay with vitamin C as a standard. The antioxidant activity increased with dose dependence of extracts (from 20 to 200µg/ml). Results: The order for reducing power of extracts was recorded as vitamin C > methanol > water ˜ chloroform > petroleum ether and the same order was found in the DPPH assay having 50% inhibitory concentration (IC50) values. The results revealed that the methanolic extract exhibited the highest percent inhibition of the reducing ability and DPPH radical scavenging assay as compared to the other extracts of both plant. Moreover, methanolic extract showed concentration dependent significant scavenging of DPPH, especially with S. indicus [IC50 values 112.23±0.39 µg/ml] and A. precatorius [IC50 values 101.44±0.41µg/ml], while that of the standard was 13.45±0.37µg/ml (vitamin C). Methanolic extract showed phenolics, flavonoids and tannins contents were significantly higher than all the extracts investigated in both plant. Conclusions: A correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant. The results suggest that methanolic leaves extract of S. indicus and A. precatorius are source of natural antioxidants and free radical scavenging capability.

Cite this article:
Ashutosh Pal Jain, Gajendra Pratap Choudhary. In vitro Antioxidant potential of Sphaeranthus indicus and Abrus precatorius leaves extracts. Research Journal of Pharmacy and Technology. 2021; 14(7):3821-5. doi: 10.52711/0974-360X.2021.00662

Ashutosh Pal Jain, Gajendra Pratap Choudhary. In vitro Antioxidant potential of Sphaeranthus indicus and Abrus precatorius leaves extracts. Research Journal of Pharmacy and Technology. 2021; 14(7):3821-5. doi: 10.52711/0974-360X.2021.00662   Available on:

1.    Aruoma OI. Free radicals, oxidative stress, and antioxidants in human health and disease. Journal of the American Oil Chemists Society. 1998; 75: 199–212.
2.    Ordonez AAL. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts Food Chemistry. 2006; 97: 452–458.
3.    Ames B. Micronutrients prevent cancer and delay aging. Toxicology Letters.1998; 102: 5–18.
4.    Fabricant DS and Farnsworth N R. The value of plants used in traditional medicine for drug discovery. Environmental health perspectives. 2001; 109(1): 69-75.
5.    Maxwell SRJ. Prospects for the use of antioxidant therapies. Drugs.1995; 49(3): 345-361.
6.    Zachariah SM et al. Phytochemical and pharmacological screening of Sphaeranthus indicus Linn. for antimicrobial activity. International Journal of Pharmaceutical Sciences and Research. 2010; 1(10): 169.
7.    Vimalanathan S et al. Medicinal plants of Tamil Nadu (Southern India) are a rich source of antiviral activities. Pharmaceutical biology. 2009; 47: 422-429.
8.    Arivoli S et al. Larvicidal activity of fractions of Sphaeranthus indicus Linnaeus (Asteraceae) ethyl acetate whole plant extract against Aedes aegypti Linnaeus 1762, Anopheles stephensi Liston 1901 and Culex quinquefasciatus Say 1823 (Diptera: Culicidae)." Int. J. Mosq. Res.2016; 3: 18-30.
9.    Nanda BK et al. Analgesic and antipyretic activity of whole parts of Sphaeranthus indicus Linn. Journal of Chemical and Pharmaceutical Research.2009; 1: 207‑12.
10.    Sundari K et al. Hepatoprotective effect of ethanolic extracts of Sphaeranthus indicus (Linn.) on paracetamol‑induced liver toxicity in rats. International Journal of Applied Biology and Pharmaceutical Technology. 2011; 2: 315‑21.
11.    Nayak SS et al. Antitussive and synergistic effects of sleeping time induced by standard sedatives of Sphaeranthus indicus plant extracts. Journal of Advanced Pharmaceutical Research.2010; 1: 123‑32.
12.    Sadaf, F et al. Healing potential of cream containing extract of Sphaeranthus indicus on dermal wounds in Guinea pigs. Journal of ethnopharmacology. 2006; 107(2): 161-163.
13.    Sarpate RV et al. Bronchodilatory effect of Sphaeranthus indicus Linn. against allergen induced bronchospasm in guinea pigs. Pharmacognosy magazine. 2009; 5(19): 74.
14.    Galani VJ and Patel BG. Effect of hydroalcoholic extract of Sphaeranthus indicus against experimentally induced anxiety, depression and convulsions in rodents. International journal of Ayurveda research. 2010; 1: 87‑92.
15.    Bafna AR and Mishra SH. Protective effect of bioactive fraction of Sphaeranthus indicus Linn. against cyclophosphamide induced suppression of humoral immunity in mice. Journal of ethnopharmacology. 2006; 104(3): 426-429.
16.    Ghaisas M et al. Preventive effect of Sphaeranthus indicus during progression of glucocorticoidinduced insulin resistance in mice. Pharmaceutical biology. 2010; 48(12): 1371-1375.
17.    Mathew JE et al. Mast cell stabilizing effects of Sphaeranthus indicus. Journal of ethnopharmacology. 2009; 122(2): 394-396.
18.    Akinloye BA and Adalumo LA. Abrus precatorius leaves-a source of glycyrrhizin. Nigerian Journal of Pharmaceutical Research. 1981; 12: 405.
19.    Nwodo OF and Alumanah EO. Studies on Abrus precatorius seeds. II: antidiarrhoeal activity. Journal of ethnopharmacology. 1991; 31(3): 395-8.
20.    Ratnasooriya WD et al. Premakumara.Sperm antimotility properties of a seed extract of Abrus Precatorius. Journal of ethnopharmacology. 1991; 33(1-2): 85–90.
21.    Taur DJ et al. Antiasthmatic related properties of Abrus precatorius leaves on various models. Journal of Traditional and Complementary Medicine. 2017; 30: 1-5.
22.    Georgewill OA and Georgewill UO. Evaluation of the anti-inflammatory activity of extract of Abrus precatorious. Eastern Journal of Medicine. 2009; 14(1): 23.
23.    Zore GB et al. Activity-directed-fractionation and isolation of four antibacterial compounds from Abrus precatorius L roots. Natural product research. 2007; 21(10): 933-940.
24.    Wakawa HY et al. Effects of Aqueous Extract of Leaves of Abrus precatorius (Linn) on some Biochemical Parameters in Normal and Alloxan Induced Diabetic Rats. Asian Journal of Biochemistry. 2017; 12(2): 44-48.
25.    Adesina SK. Studies on some plants used as anticonvulsants in Amerindian and African traditional medicine. Fitoterapia. 1982; 53: 147–162.
26.    Ghosh D and Maiti TK. Immunomodulatory and anti-tumor activities of native and heat denatured Abrus agglutinin. Immunobiology. 2007; 212(7): 589-599.
27.    Palvai VR et al. Abrus precatorius Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability. International Journal of Medicinal Chemistry. 2014; 1-7.
28.    Winterbourn CC. Nutritional antioxidants: their role in disease prevention. The New Zealand medical journal.1995; 108(1011): 447-449.
29.    Folin O and Ciocalteu V. On tyrosine and tryptophane determinations in proteins. 1927; Journal of biological chemistry. 73 (2): 627-650.
30.    Makkar HPS et al. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture. 1993; 61: 161–165.
31.    Olajuyigbe OO and Afolayan, AJ. Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata Willd. subsp. mucronata Willd. BMC complementary and alternative medicine. 2011; 11(1): 130.
32.    Sunil C and Ignacimuthu S. In vitro and in vivo antioxidant activity of Symplocos cochinchinensis S. Moore leaves containing phenolic compounds. Food and chemical toxicology. 2011; 49(7): 1604-1609.
33.    Nikolova M.Screening of radical scavenging activity and polyphenol content of Bulgarian plant species.Pharmacognosy research. 2011; 3(4): 256.
34.    Larson RA. The antioxidants of higher plants. Phytochemistry. 1988; 27(4): 969-978.
35.    Kumar S and Pandey AK. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal. 2013; 2013.
36.    Meir S et al. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of agricultural and food chemistry. 1995; 43(7): 1813-1819.
37.    Özen T and Kinalioğlu, K. Determination of antioxidant activity of various extracts of Parmelia saxatilis. Biologia. 2008; 63(2): 211-216.
38.    Matthäus B. Antioxidant activity of extracts obtained from residues of different oil seeds. Journal of Agricultural and Food Chemistry. 2002; 50(12): 3444-3452.

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