T. P. Dugawale, C.C. Khanwelkar, P.P. Durgawale
T. P. Dugawale1*, C.C. Khanwelkar2, P.P. Durgawale3
1Ph.D. Candidate, Department of Pharmacology, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
2Professor and Head, Department of Pharmacology, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
3Research Officer, Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
Volume - 14,
Issue - 7,
Year - 2021
P. oleracea and P. quadrifida are closely related species belonging to family Portulaceae. They are found in almost parts of the globe growing as neglected weed. However, recent interest in these plants owing to their palatable sour taste and health benefits have made them acceptable as cultivable plants and potted herbs. Even though numerous studies indicate the presence of pharmacological compounds in these plants, reports on quality assurance practices followed for evaluating these plants as crude drugs have not been detailed. In this study, the plant species were harvested as weed, authenticated, extracted using different solvents and extraction techniques. The extracts were then evaluated and studied for their primary phytochemical composition. This was followed by isolation of beta sitosterol using thin layer chromatography and column chromatography. The results indicate the presence all major classes of phytochemicals such as steroids, flavanoids, alkaloids, saponins, polysaccharides, and tannins. The percentage yield, extractive values, ash values, moisture content were calculated for both the species. The isolation of beta-sitosterol may be used as a quality assurance procedure. The data presented in this study may be further used by researchers for primary evaluation of quality of crude drugs in the form of P. oleracea and P. quadrifida plants.
Cite this article:
T. P. Dugawale, C.C. Khanwelkar, P.P. Durgawale. Primary Phytochemical Evaluation of P. oleracea and P. quadrifida. Research Journal of Pharmacy and Technology. 2021; 14(7):3789-3. doi: 10.52711/0974-360X.2021.00656
T. P. Dugawale, C.C. Khanwelkar, P.P. Durgawale. Primary Phytochemical Evaluation of P. oleracea and P. quadrifida. Research Journal of Pharmacy and Technology. 2021; 14(7):3789-3. doi: 10.52711/0974-360X.2021.00656 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-53
1. Masoodi MH, Ahmad B, Mir SR, Zargar BA, Tabasum N. Portulaca oleracea L. a review. Journal of Pharmacy Research. 2011; 4(9): 3044-8.
2. Kamal-Uddin MD, Juraimi AS, Begum M, Ismail MR, Rahim AA, Othman R. Floristic composition of weed community in turf grass area of west peninsular Malaysia. International Journal of Agriculture and Biology. 2009; 1; 11(1): 13-20.
3. Uddin MK, Juraimi AS, Ismail MR, Brosnan JT. Characterizing weed populations in different turfgrass sites throughout the Klang Valley of Western Peninsular Malaysia. Weed Technology. 2010; 24(2): 173-81.
4. Uddin M, Juraimi AS, Hossain MS, Nahar M, Un A, Ali M, Rahman MM. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. The Scientific World Journal. 2014; 2014.
5. Syed S, Fatima N, Kabeer G. Portulaca oleracea L.: a mini review on phytochemistry and pharmacology. International journal of Biology and Biotechnology. 2016; 13(4): 637-41.
6. Uddin M, Juraimi AS, Ali M, Ismail MR. Evaluation of antioxidant properties and mineral composition of purslane (Portulaca oleracea L.) at different growth stages. International journal of molecular sciences. 2012; 13(8): 10257-67.
7. Kamil MS, Ahmed MD, Paramjyothi S. Neuropharmacological effects of ethanolic extract of Portulaca quadrifida Linn. in mice. International Journal of Pharm Tech Research. 2010; 2(2): 1386-90.
8. Mulla SK, Swamy P, 2010. Preliminary pharmacognostical and phytochemical evaluation of Portulaca quadrifida Linn. Int J Pharm Tech Res, 2, 3, 1699-1702.
9. Sukhdev SH, Suman PS, Gennaro L, Dev DR. Extraction technologies for medicinal and aromatic plants. United Nation Industrial Development organization and the international center for Science and High Technology. 2008; 116.
10. Majekodunmi SO. Review of extraction of medicinal plants for pharmaceutical research. Merit Res J Med Med Sci 2015. 2015; 3: 521-7.
11. Yao H, Li X, Liu Y, Wu Q, Jin Y. An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L. Journal of Ginseng Research. 2016 1; 40(4): 415-22.
12. Bonomini TJ, Góes JA, Machado MD, da Silva RM, Malheiros A. development and optimization of a microwave-assisted extraction of plumieride from Allamanda cathartica L. flowers. Química Nova. 2018; 41(1): 36-42.
13. Li Y, Li S, Lin SJ, Zhang JJ, Zhao CN, Li HB. Microwave-assisted extraction of natural antioxidants from the exotic Gordonia axillaris fruit: Optimization and identification of phenolic compounds. Molecules. 2017 6; 22(9): 1481.
14. Tomaniova M, Hajšlová J, Pavelka Jr J, Kocourek V, Holadova K, Klımova I. Microwave-assisted solvent extraction-a new method for isolation of polynuclear aromatic hydrocarbons from plants. Journal of Chromatography A. 1998 4; 827(1): 21-9.
15. Nana O, Momeni J, Boyom FF, Ngassoum MB. Influence of microwave assisted extraction on antioxidant and antiplasmodial activities of Trichilia roka extracts. The Journal of Phytopharmacology. 2015; 4(5): 248-252
16. Uttara J, Leena W, Mohini U. Microwave assisted extraction of crude drugs. Int J Pharm Bio Sci. 2010; 1: 330-2.
17. Norfaezah A, Zainab H, Othman H. Comparative study between microwave assisted extraction and soxhlet extraction techniques for bio-oil extraction from Jatropha curcas. Journal of Engineering Science and Technology. 2015; 1;10: 9-16.
18. Patil SG, Wagh AS, Pawara RC, Ambore SM. Standard tools for evaluation of herbal drugs: an overview. The Pharma Innovation. 2013; 1; 2(9).
19. Khandelwal K R, Practicle pharmacognosy Nirali Prakashan Pune 3rd Edition. Page 150-156,160.
20. Sutar RC, Kasture SB, Kalaichelvan VK. Identification, quantification and validation of β-sitosterol from Holoptelea integrifolia (roxb.) planch using high performance thin layer chromatography method. Int. J. Pharm. Pharm. Sci. 2014; 6(5): 249-52.
21. Harborne J B, Phytochemical methods, a guide to modern techniques of plant analysis, Springer 3rd ed 2003: 4-10; 217.
22. Dhole JA, Dhole NA, Lone KD, Bodke SS, Dhole JA. Preliminary phytochemical analysis and antimicrobial activity of some weeds collected from Marathwada region. Journal of research in Biology. 2011; 1: 19-23.