Author(s):
Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar
Email(s):
pdeepasankar@vit.ac.in
DOI:
10.52711/0974-360X.2021.00655 
Address:
Saumya S1, Shalini S1, S. Leelavathy1, P. Deepa Sankar2*
1School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.
2VIT School of Agricultural Innovations and Advanced Learning (VAIAL), Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 7,
Year - 2021
ABSTRACT:
Objective: The study focuses on formulating and validating low-cost media for in vitro callus induction and development in Catharanthus roseus. Methods: Percentage response, nature and mass of in vitro grown C. roseus callus was recorded for the conventional MS media with casein hydrolysate (1g/L) and three low-cost media supplemented with casein hydrolysate (1g/L). The effect of two hormonal combinations, viz., 1.5mg/L 2,4-D + 1mg/L Kin and 1mg/L BAP + 1mg/L NAA had been studied for callus development. Results: Two media combinations including MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1 g/L) + Food grade agar (FGA, 7g/L) and MS + table sugar (30g/L) + casein hydrolysate (1g/L) + sago (80g/L) was observed to take less number of days to callus induction, 11 and 12 days respectively, than the conventional MS media + casein hydrolysate (1g/L) + gelrite (2.2g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L), which took 13 days to callus induction. MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) gave rise to high callus mass, averaging to 12.351g, at the end of 4th subculture and also, high percentage of cost reduction per liter of media was observed compared to conventional MS media with casein hydrolysate. Conclusion: The present study indicated MS (urea replacing NH4NO3) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) as a better media for developing and proliferating C. roseus callus In vitro.
Cite this article:
Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar. Low-Cost Media for In-vitro Callus Development in Catharanthus roseus (L) G. Don. Research Journal of Pharmacy and Technology. 2021; 14(7):3785-8. doi: 10.52711/0974-360X.2021.00655
Cite(Electronic):
Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar. Low-Cost Media for In-vitro Callus Development in Catharanthus roseus (L) G. Don. Research Journal of Pharmacy and Technology. 2021; 14(7):3785-8. doi: 10.52711/0974-360X.2021.00655 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-52
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