Author(s):
Dharani Dharan, K. Venkatesh, S.S. Meenambiga, Dhivya Dhanasekar, P. Arumugam
Email(s):
meenambiga.se@velsuniv.ac.in
DOI:
10.52711/0974-360X.2021.00653
Address:
Dharani Dharan1, K. Venkatesh1, S.S. Meenambiga1*, Dhivya Dhanasekar2, P. Arumugam2
1Department of Bio-Engineering, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai – 600117.
2ARMATS Biotek Training and Research Institute, Guindy, Chennai, Tamil Nadu – 600032.
*Corresponding Author
Published In:
Volume - 14,
Issue - 7,
Year - 2021
ABSTRACT:
The presented work represents phytochemical analysis, Antioxidant assay and Antimicrobial activity of Bhut jolokia pepper (Capsicum chinense Jacq) extracted by ethanol as solvent. The ethanolic extract of Bhut jolokia pepper showed the presence of terpenoids, steroids, saponins and flavonoids. Antimicrobial assay was done with varying concentration (250-1000µg/ml) of pepper extract using tetracycline as control by well diffusion method, the extract at 750µg/ml shown best inhibition zone and Staphylococcus aureus showed the highest zone of inhibition at all concentration compared to other bacteria species with maximum zone of inhibition of 27mm. The DPPH scavenging assay for antioxidant activity at 517nm showed positive activity for scavenging, scavenging increased with the concentration of extract. Thus, Bhut jolokia could be effective in treating diseases caused by Staphylococcus aureus from antimicrobial assay result.
Cite this article:
Dharani Dharan, K. Venkatesh, S.S. Meenambiga, Dhivya Dhanasekar, P. Arumugam. Phytochemical analysis, Antimicrobial and Antioxidant assay of Bhut Jolokia pepper. Research Journal of Pharmacy and Technology. 2021; 14(7):3775-8. doi: 10.52711/0974-360X.2021.00653
Cite(Electronic):
Dharani Dharan, K. Venkatesh, S.S. Meenambiga, Dhivya Dhanasekar, P. Arumugam. Phytochemical analysis, Antimicrobial and Antioxidant assay of Bhut Jolokia pepper. Research Journal of Pharmacy and Technology. 2021; 14(7):3775-8. doi: 10.52711/0974-360X.2021.00653 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-50
REFERENCES:
1. Bosland PW, Baral JB. ‘Bhut Jolokia’—The world's hottest known chile pepper is a putative naturally occurring interspecific hybrid. Hort Science. 2007;42(2): 222-224.
2. Bosland PW, Coon D, Reeves G. ‘Trinidad Moruga Scorpion’ pepper is the world’s hottest measured chile pepper at more than two million Scoville heat units. Hort Technology. 2012; 22(4): 534-538.
3. Michaud M, Michaud J. Wild Thing: The Naga Morich Story. In Vegetables: Proceedings of the Oxford Symposium on Food and Cooking 2008 2009 (Vol. 26). Oxford Symposium.
4. Nelson EK, Dawson LE. The constitution of capsaicin, the pungent principle of Capsicum. III. Journal of the American Chemical Society. 1923;45(9): 2179-2181.
5. Mena et al. Evaluation of capsaicin, Ascorbic acid, α-Carotene and ß-Carotene in Bhut Jolokia (Capsicum chinense Jacq.) genotypes from North East India.
6. Tiwari et al. Adaptability and production of hottest chilli variety under Gwalior agro-climatic conditions. Current Science. 2005; 88(10): 1545-1546.
7. Brito-Argáez et al. Characterization of a Capsicum chinense seed peptide fraction with broad antibacterial activity. Asian Journal of Biochemistry. 2009;4(3): 77-87.
8. Omolo et al. Antimicrobial properties of chili peppers. Journal of Infectious Diseases and Therapy. 2014 Jun 6.
9. Baruah et al. A review on recent researches on Bhut jolokia and pharmacological activity of capsaicin. International Journal of Pharmaceutical Sciences Review and Research. 2014;24(2): 89-94.
10. Bhagowati RR, Changkija S. Genetic variability and traditional practices in Naga King Chili landraces of Nagaland. Asian Agri-History. 2009;13(3): 171-180.
11. Arora et al. An overview about versatile molecule capsaicin. Int J Pharm Sci Drug Res. 2011;3(4): 280-286.
12. Ahuja KD, Ball MJ. Effects of daily ingestion of chilli on serum lipoprotein oxidation in adult men and women. British journal of nutrition. 2006;96(2): 239-242.
13. Adams MJ, Ahuja KD, Geraghty DP. Effect of capsaicin and dihydrocapsaicin on in vitro blood coagulation and platelet aggregation. Thrombosis research. 2009;124(6): 721-723.
14. Yang et al. Capsaicin mediates cell death in bladder cancer T24 cells through reactive oxygen species production and mitochondrial depolarization. Urology. 2010;75(3): 735-741.
15. Thoennissen et al. Capsaicin causes cell-cycle arrest and apoptosis in ER-positive and-negative breast cancer cells by modulating the EGFR/HER-2 pathway. Oncogene. 2010;29(2): 285-296.
16. Okumura et al. Effect of caffeine and capsaicin on the blood glucose levels of obese/diabetic KK-Ay mice. Journal of oleo science. 2012;61(9): 515-523.
17. Hassan et al. Antioxidant and antiapoptotic effects of capsaicin against carbon tetrachloride-induced hepatotoxicity in rats. Toxicology and industrial health. 2012;28(5): 428-438.
18. Meeran SB, Subburaya U, Narasimhan G. In Silico and In Vitro Screening of Ethanolic Extract of Fruits of Withania coagulans against Diabetes. Research Journal of Pharmacy and Technology. 2020;13(2): 631-635.
19. Trease GE, Evans WC. Trease and Evanspharmacognosy 14th edition Suvnder Company Limited. London Pp191-293. 1996.
20. Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology. 1998;62(2): 183-193.
21. Salama et al. In-vitro Antioxidant, Antimicrobial and Anticancer Activities of Banana leaves (Musa acuminata) and Olive leaves (Olea europaea L.) as by-products. Research Journal of Pharmacy and Technology. 2020;13(2): 687-696.
22. Raj KJ, Shalini K. Flavonoids-a review of biological activities. 1999; 36(1): 668-676.
23. Saidu AN, Garba R. Antioxidant activity and phytochemical screening of five species of capsicum fruits. International Research Journal of Biochemistry and Bioinformatics. 2011;1(9): 237-241.
24. Gayathri N, Gopalakrishnan M, Sekar T. Phytochemical screening and antimicrobial activity of Capsicum chinense Jacq. International Journal of Advances in Pharmaceutics. 2016;5(1): 12-20.
25. Shibi et al . Antimicrobial and Anti-cancer Activity of Capsicum chinense Bhut Jolokia fruit: An In-vitro Analysis. International Journal of Current Science Research. 2017:3(12): 1458-1466.
26. Chhapekar SS, Ahmad I, Abraham SK, Ramchiary N. Analysis of bioactive components in Ghost chili (Capsicum chinense) for antioxidant, genotoxic, and apoptotic effects in mice. Drug and Chemical Toxicology. 2018 Jul:1(0): 182-191.
27. Sarpras M, Gaur R, Sharma V, Chhapekar SS, Das J, Kumar A, Yadava SK, Nitin M, Brahma V, Abraham SK, Ramchiary N. Comparative analysis of fruit metabolites and pungency candidate genes expression between Bhut Jolokia and other Capsicum species. PLoS One. 2016:11(12): 1-19.
28. Choudhary RK, Saroha AE, Swarnkar PL. Radical Scavenging Activity of Phenolics and Flavonoids in Some Medicinal Plants of India. J. Pharm. Res. 2011;4(3): 712-713.
29. Das J, Deka M, Gogoi K. Antimicrobial Activity of Chilli Extracts (Capsicum chinense) Against Food Borne Pathogens Escherichia coli and Staphylococcus aureus. IJRAR-International Journal of Research and Analytical Reviews. 2018;5(4): 717-720.
30. Agarwal et al. Antimicrobial property of capsaicin. Inter. Res. J. Biol. Sci. 2017;6(7): 7-11.
31. Amruthraj NJ, Raj JP, Lebel LA. Polar Aprotic Extraction of Capsaicinoids from Capsicum chinense Bhut jolokia fruit for Antimicrobial activity. International Journal of Biological and Pharmaceutical Research. 2013;4(12): 959-964.