Author(s): D. Pooja Reddy, S.B. Bhanja, Ashwini K Chauhan, B. Kranthi Kumar, Dibya Sunder Panda, B.B Panigrahi


DOI: 10.52711/0974-360X.2021.00642   

Address: D. Pooja Reddy1*, S.B. Bhanja1, Ashwini K Chauhan1, B. Kranthi Kumar1, Dibya Sunder Panda2, B.B Panigrahi3
1Department of Pharmaceutics, Malla Reddy College of Pharmacy, (Affiliated to Osmania University), Maisammaguda, Hyderabad, Telangana State, India.
2College of Pharmacy, Jouf University, Kingdom of Saudi Arabia.
3Hi-Tech College of Pharmacy, Bhubaneswar, Odisha.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021

At present, synthetic drugs form a major line of treatment in the management of many diseases and currently available as transdermal patches. Traditional medicine system is centuries old practice and again gaining importance. Hence, herbal products can be used to treat many diseases as transdermal patches. Neem leaves has antibacterial properties and can be used for controlling air borne bacterial contamination. Azadirachta Indica. A. Juss (neem) very useful traditional plant. The present study was carried out to extract, formulate and evaluate a transdermal patches containing Azadirachta indica A. Juss The total four Transdermal patches were prepared by solvent casting method and evaluated for physicochemical characteristics such as weight variation, thickness, drug content uniformity, folding endurance, In-vitro diffusion, ex-vivo permeation studies and anti-Bacterial screening test. The infrared spectroscopy showed that there was no incompatibility between drug and polymer. The In-vitro diffusion studies of Transdermal patches of Neem showed percentage of drug release from 65.2% to 92.06% at the end of 21hrs. The ex-vivo permeation study was carried out for optimized formulation (M2) using goat abdomen skin as barrier and showed percentage drug release 89.6% at the end of 21hrs. Release kinetics data showed that all the formulations followed zero order kinetics with non-Fickian diffusion mechanism. The anti-bacterial screening study showed good anti-bacterial activity against Bascillus subtilis and Pseudomonas aeruginosa and zone of Inhibition (ZOI) was compared against standard antibiotic drugs i.e. Penicillin and Streptomycin.

Cite this article:
D. Pooja Reddy, S.B. Bhanja, Ashwini K Chauhan, B. Kranthi Kumar, Dibya Sunder Panda, B.B Panigrahi. Methanolic Extraction, Formulation and Evaluation of Herbal Transdermal Patches of Azadirachta indica A. Juss. Research Journal of Pharmacy and Technology. 2021; 14(7):3709-5. doi: 10.52711/0974-360X.2021.00642

D. Pooja Reddy, S.B. Bhanja, Ashwini K Chauhan, B. Kranthi Kumar, Dibya Sunder Panda, B.B Panigrahi. Methanolic Extraction, Formulation and Evaluation of Herbal Transdermal Patches of Azadirachta indica A. Juss. Research Journal of Pharmacy and Technology. 2021; 14(7):3709-5. doi: 10.52711/0974-360X.2021.00642   Available on:

1.    Karunamoorthi K, Jegajeevanram K, Jerome X, Vijayalakshmi J, Tamil traditional medicinal system - Siddha: an indigenous health practice in the international perspectives. Int J Genu Tradi Medi. 2(2); 2012: 111.
2.    Legal status of traditional medicine and complementary/ alternative medicine: a worldwide review (document who/edm/trm/2001.2). Geneva, world health organization, 2001
3.    Dawit A, Traditional medicine in Ethiopia: the attempt being made to promote it for effective and better utilization. SINET. Ethiopian J Sci .9(4); 1986: 61-69.
4.    Kong JM, Goh NK, Chia LS, Chia TF, Recent advances in traditional plant drugs and orchids. Acta Pharmacol Sin.4 (2); 2003: 7-21.
5.    Joy PP, Thomas J, Mathew S, Skaria BP Medicinal plants, Tropical Horticulture. Kolkata India. 2(4); 2001: 1-12.
6.    Hamsaveni G, Saraswathi S, Purushotaman K.K, Antimalarials from Indian medicinal plants. J Res in Ayurveda Siddha.2 (3); 1981: 286295.
7.    Ravishankar S, Indian Systems of Medicine: A Brief Profile Complementary and Alternative Medicines V.J. African. J Tradi medi .4 (3); 200: 319 – 337.
8.    Qazi majaz A, Molvi khurshid I, Herbal medicine: a comprehensive review. Int J Pharma Res. 8(2); 2016: 1-5.
9.    Alok Maithani1, Versha P, Geeta P, Ishan D, A Review Introduction Azadirachta indica (Neem) Leaf, J Pharma Res.4(6);2011: 1824-1827.
10.    Sayan B, Nagalakshmi S, Formulation characterization and in-vitro diffusion studies of herbal extract loaded mucoadhesive buccal patches, Ind J Pharma Sci Res.5(11); 2014: 4965-497.
11.    Itelima, Nwokedi V.C., Ogbonna A.I., Nyam M.A., Phytochemical Screening and Antimicrobial Activity, Evaluation of Aqueous and Ethanolic Extracts of The Leaf of Azadirachta indica Juss (Neem) On Some Microorganisms, J Pharma Sci.3(1); 2016: 056-060.
12.    Mariana, Carlos H. G, Jaqueline Ma, Taís M.B, Phytochemical Screening of Azadirachta indica A. Juss For Antimicrobial Activity, Ind J Pharmal Sci Res.11(4);2017: 3617-36122.
13.    Mitali G, Formulation and evaluation of transdermal patch of an antihypertensive drug. The International J. Pharm Sci .4(3); 2013: 3456-3682
14.    Sanjay, et al, Preparation of cervediol transdermal patches and effect of propylene glycol permeation. Int Pharm and Pharm Sci. 2(1); 2010: 137-143
15.    Satyabrata B, Brij Mohan Singh R, Sudhakar M, Panigrahi BB, Design, Development and Evaluation of Transdermal Patches of Ramipril, Int J Pharma Sci Res.3(2);2014: 350-360.
16.    Wahid, Sridhar BK, Sivakumar S, Preparation and evaluation of transdermal drug delivery system of etoricoxib using modified chitosan, Ind J Pharma Sci. 70(4);2008: 55-60.
17.    Srinivas, Nayanabhirama U. Formulation development, in vitro and ex vivo evaluation of membrane-controlled systems of glibenclamide. Ind J Pharmal Sci. 8(5); 2005: 26-38.
18.    Sheba R, Nakka D, Rajan R, Eu Sheau Z, Development and In vitro Evaluation of Self-Adhesive Matrix-Type Transdermal Delivery System of Ondansetron Hydrochloride. Trop J Pharma Res.14 (2); 2015: 211-218.
19.    Cherukuri S, Rajeswari B, Kiranmai M, Formulation and evaluation of transdermal drug delivery of topiramate. Int J Pharma Invest. 7(1); 2017:1-8.
20.    Tanwar, et al, Development and evaluation of cervediol transdermal patches. Acta Pharm. 5(1);2001: 151-159.
21.    Paulo Costa, et al, Modeling and comparison of dissolution profiles. Euro J Pharm Sci.1(3);2001: 1233-1333
22.    Mukherge, et al, Design Development Physicochemical and in–vitro, ex-vivo, Evaluation of Transdermal patches containing Diclofenac Diethyl Ammonium salt. Journal of Pharmaceutical Sciences. 91; 2002: 2076-2089.
23.    Parthasarathy H, Smruti T, Evaluation of Antimicrobial Activity of Azadirachta Indica, Syzygium aromaticum and Cinnamomum zeyalnicum Against Oral Microflora, Asian J Exp Sci.27(2); 2013:1316.
24.    Soniya A, Vidya P, Maji Jose M.D.S, Antimicrobial Potential of the extracts of the twigs of Azadirachta indica (Neem): An in -vitro study. J Medi Plants Studies.2 (6); 2014: 53-57.
25.    Mariana C, Carlos H.G, Jaqueline M, Taís M.B, Phytochemical screening of Azadirachta indica A. Juss For Antimicrobial Activity. J Medi Plants Studies.11(4);2017: 117-12
26.    Lalita Chauhan, et al, Formulation and evaluation of Novel herbal antidiabetic transdermal patch, Inno Pharm Pharmacother. 6(4); 2018:61-64

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