Author(s):
Dahliatul, Qosimah, Sri Widyarti, Beltran MAG, Muhaimin, Rifa’i
Email(s):
rifa123@ub.ac.id
DOI:
10.52711/0974-360X.2021.00638 
Address:
Dahliatul, Qosimah1, Sri Widyarti2, Beltran MAG3, Muhaimin, Rifa’i2
1Laboratory of Mikrobiology dan Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Indonesia.
2Laboratory of Animal Physiology, Structure, Growth, Faculty of Science, Universitas Brawijaya, Indonesia.
3College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac, Philippines.
*Corresponding Author
Published In:
Volume - 14,
Issue - 7,
Year - 2021
ABSTRACT:
An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease
characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both. Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice. An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice were sacrificed Furthermore, the pancreas was taken for insulin examination, and the spleen was checked with the relative number of CD11b-IL6 and T CD4 cells using a flow cytometer. Data were quantitatively analyzed using the One Way ANOVA test (p=0.05). The results showed that doses of coriander in T1 treatment causes an increase in insulin levels, decreased blood sugar levels, weight gain, proliferation and activation of CD4+ T cells, and decrease inflammation (CD11b cell-IL6) on the tissue. This research concludes that extracts of coriander can reduce Hyperglycemia in mice by modulation of cellular immunity and increased levels of insulin
Cite this article:
Dahliatul, Qosimah, Sri Widyarti, Beltran MAG, Muhaimin, Rifa’i. Improved cellular immunity and increased insulin in streptozotosin-induced mice using ethanol coriander (Coriandrum sativum) extract. Research Journal of Pharmacy and Technology. 2021; 14(7):3689-4. doi: 10.52711/0974-360X.2021.00638
Cite(Electronic):
Dahliatul, Qosimah, Sri Widyarti, Beltran MAG, Muhaimin, Rifa’i. Improved cellular immunity and increased insulin in streptozotosin-induced mice using ethanol coriander (Coriandrum sativum) extract. Research Journal of Pharmacy and Technology. 2021; 14(7):3689-4. doi: 10.52711/0974-360X.2021.00638 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-35
REFERENCES:
1. Al-Snafi, A.E. A review on chemical constituents and pharmacological activities of Coriandrum sativum. OSR Journal of Pharmacy. 2013; 6(7): 17-42
2. Bahadoran Z, Mirmiran P, Azizi F. Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. Journal of Diabetes and Metabolic Disorders. 2013; 12:43
3. Bhat SP, Kumar WRA. Oriandrum Sativum on Pain and Inflammation. JRPC. 2014; 4(4): 939-945
4. Chizoba EF. A Comprehensive Review on Coriander and its Medicinal properties. International Journal of Scientific Research and Reviews. 2015; 4(2): 28 – 50
5. Damasceno DC, Netto AO, Iessi I., Gallego F, Corvin, SB, Dallaqua, B, Sinzato YK, Bueno A, Calderon MP, Rudge MVC. Streptozotocin-Induced Diabetes Models: Pathophysiological Mechanisms and Fetal Outcomes. Biomed Res Int. 2014; 819065.
6. Deepa B, Anuradha CV. Antioxidant Potential of Coriandrum sativum L Seed Extract. Indian Journal of Experimental Biology. 2011; 4930: 38
7. Frederico EHFF, Cardoso, A.L.B.D., Guimarães, C.A.S., Neves, R.F., Sá-Caputo, D.C., Moreira-Marconi, E., Dionello, C.F., Morel, D., Paineiras-Domingos, L.L., Costa-Cavalcanti, R.G., Sousa-Gonçalves, C.R., Arnóbio, A and Bernardo-Filho, M. 2016. Possible Benefits of the Coriandrum sativum in the Management of Diabetes in Animal Model: A Systematic Review Herb Med
8. Furman BL. Streptozotocin-Induced Diabetic Models in Mice and Rats. Current Protocols in Pharmacology. 2015; 5.47.1-5.47.20
9. Gomes KB. IL-6 and type 1 diabetes mellitus: T cell responses and increase in IL-6 receptor surface expression. Ann Transl Med. 2017; 5(1): 16
10. Güler T, Ertaş ON, Çiftç M, Dalkılıç B. The effect of coriander seed (Coriandrum sativum L.) as diet ingredient on the performance of Japanese quail. South African Journal of Animal Science. 2005; 35 (4)
11. Gvazava IG, Rogovaya OS, Borisov M., Vorotelyak EA, Vasiliev AV. Pathogenesis of Type 1 Diabetes Mellitus and Rodent Experimental Models. Acta Naturae. 2018; 10(1): 24–33.
12. Hackett E, Jacques N. Tipe 2 Diabetes Pathophysiology and Clinical Features. Clinical Pharmacist. 2009; 1
13. Hossain K., Dayem AA, Han J, Yin Y, Kim K, Saha SK, Gwang-Mo Y, Choi, HY, Ssang-Goo Cho. Molecular Mechanisms of the Anti-Obesity and Anti-Diabetic Properties of Flavonoids. International Journal of Molecular Sciences. 2016; 17, 569
14. Hajiaghaalipour MF, Khalilpourfarshbafi M, Arya A. Modulation of Glucose Transporter Protein by Dietary Flavonoids in Type 2 Diabetes. International Journal of Biological Sciences. 2015; 11(5): 508-524.
15. Kakoolaa DN, Curcio-Brinta A, Lenchika N, Gerling IC. Molecular pathway alterations in CD4 T-cell of non obesediabetic (NOD) mice in the pre insulitis phase of autoimmune diabetes. Results in Immunology. 2014; 4. 30-45
16. Kawata A, Yukio M, Seiji S and Seiichiro F. Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species. In vivo. 2018; 32: 255-264
17. Nair V, Singh S, Gupta YK. Evaluation of disease modifying activity of Coriandrum sativum in experimental models. Indian J Med Res. 2012; 135(2): 240–245.
18. Qosimah D, Dhita EA, Ma AGB, Aulanni’am A. Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus. Veterinary World. 2019; 12(6): 849-854.
19. Rachmawati I, M. Rifa’i. In Vitro Immunomodulatory Activity of Aqueous Extract of Moringa oleifera Lam. Leaf to the CD4 +, CD8+ and B220+ Cells in Mus musculus. J. Exp. Life Sci. 2014; 4:1
20. Schaschkow A, Mura C, Dal S, Langlois A, Seyfritz E, Sookhareea C, Bietiger W., Peronet C, Jeandidier N, Pinget M, Sigrist S, Maillard E. Impact of the Type of Continuous Insulin Administration on Metabolism in a Diabetic Rat Model. Journal of Diabetes Research. 2016; Article ID 8310516, 10 pages.
21. Sharma B, Joshi SC, Jasuja ND, Singh SK, Gaurav S. Evaluation of Anti-Diabetic and Hepatoprotectivity of Coriandrum Sativum In Alloxan Induced Experimental Animals: A Histopathology Study. International Journal of Pharmaceutical Sciences And Research. 2016; 7(11): 4510-4515
22. Sogara PPU, Fatimawali, Bodhi W. Pengaruh Ekstrak Etanol Buah Ketumbar (Coriandrum sativum L.) Terhadap Penurunan Kadar Gula Darah Tikus Putih Yang Diinduksi Aloksan. Pharmacon Jurnal Ilmiah Farmasi – UNSRAT. 2014; 3: 3
23. Tai N, Wong FS, Wen L. The role of gut microbiota in the development of type 1, obesity and type 2 diabetes mellitus. Rev Endocr Metab Disord. 2015; 16(1): 55–65
24. Tian HL, Xu ZV, Wei LS, Zhao RT. Correlations between blood glucose level and diabetes signs in streptozotocin-induced diabetic mice. Global Journal of Pharmacology. 2010; 4(3): 111-116
25. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutr Metab (Lond). 2015; 12: 60.
26. Xia C, Rao X, Zhong J. Role of T Lymphocytes in Type 2 Diabetes and Diabetes-Associated Inflammation. J Diabetes Res. 2017: 6494795.
27. Wangchuk P, Apte SH, Smout MJ, Groves PL, Loukas A, Doolan DL. Defined Small Molecules Produced by Himalayan Medicinal Plants Display Immunomodulatory Properties. Int J Mol Sci. 2018; 19(11): 3490.
28. Wang-Fische Y and Tina G. Improving the Reliability and Utility of Streptozotocin-Induced Rat Diabetic Model. Research Article. Journal of Diabetes Research. 2018, Article ID 8054073, 14 pages
29. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 2004; 27(5):1047-53.