Author(s): Akram Nezam, Dima Al Diab, Nouma Hasan

Email(s): akramyn99@gmail.com

DOI: 10.52711/0974-360X.2021.00637   

Address: Akram Nezam1*, Dima Al Diab1, Nouma Hasan2
1Department of Analytical and Food Chemistry, Faculty of Pharmacy, Tishreen University, Latakia, Syria.
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tishreen University, Latakia, Syria.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021


ABSTRACT:
Fruit juices are considered as an important source of phenolic compounds. Black mulberry, pomegranate and orange juices were selected to assess their anti-inflammatory activity. Total phenolic content was determined using Folin-Ciocalteu colorimetric method. The anti-inflammatory activity was evaluated using RBC membrane stabilization method. Ibuprofen was used as a standard drug. Total phenolic content of black mulberry juice, pomegranate juice and orange juice was 3.02 gGAE/l, 1.83 gGAE/l and 0.76 gGAE/l and the percentage inhibition of RBC haemolysis varied between (27.41-43.19%), (24.06-39.06%) and (10.12-22.54%), respectively. Black mulberry juice revealed the highest anti-inflammatory activity among the studied juices. This finding might be attributed to the high phenolic content of this juice compared to others.


Cite this article:
Akram Nezam, Dima Al Diab, Nouma Hasan. In-vitro Anti-inflammatory activity of Total Phenolic content of some fruit juices in Syria. Research Journal of Pharmacy and Technology. 2021; 14(7):3685-8. doi: 10.52711/0974-360X.2021.00637

Cite(Electronic):
Akram Nezam, Dima Al Diab, Nouma Hasan. In-vitro Anti-inflammatory activity of Total Phenolic content of some fruit juices in Syria. Research Journal of Pharmacy and Technology. 2021; 14(7):3685-8. doi: 10.52711/0974-360X.2021.00637   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-34


REFERENCES:
1.    Chen, L., et al., Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 2018. 9(6): p. 7204.
2.    Medzhitov, R., Inflammation 2010: new adventures of an old flame. Cell, 2010; 140(6): 771-776.
3.    Cai, C., et al., Anti-inflammatory activity of N-butanol extract from Ipomoea stolonifera in vivo and in vitro. PloS one, 2014; 9(4).
4.    Murakami, M. and T. Hirano, The molecular mechanisms of chronic inflammation development. Frontiers in immunology, 2012; 3: 323.
5.    Freire, M.O. and T.E. Van Dyke, Natural resolution of inflammation. Periodontology 2000, 2013; 63(1): 149-164.
6.    Siju, P., et al., In-Vitro Anti-inflammatory Activity of Fractions of Ailanthus excelsa Roxb. by HRBC Membrane Stabilization. Asian Journal of Pharmacy and Technology, 2015; 5(1): 29-31.
7.    Sahu, R.K., et al., Anti-inflammatory action of Ougeinia oojeinensis (Roxb.) Hochr. bark by HRBC membrane stabilization. Research Journal of Pharmacy and Technology, 2008; 1(1): 57-58.
8.    Roy, A. and R.K. Sahu, Screening of Anti-inflammatory Potential of Berberis coriaceae Leaves by HRBC Membrane Stabilization. Research Journal of Pharmacology and Pharmacodynamics, 2012; 4(1): 5-6.
9.    Leelaprakash, G. and S.M. Dass, Invitro anti-inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development and Research, 2011; 3(3): 189-196.
10.    Navale, G., et al., Membrane Stabilization assay for Anti-inflammatory activity yields misleading results for samples containing traces of Methanol. Asian Journal of Pharmaceutical Research, 2019; 9(3): 169-171.
11.    Gorla, U.S., et al., Evaluation of anti-inflammatory activity of Hydroalcoholic extract of Ananas cosmosus fruit peel by HRBC membrane stabilisation. Asian Journal of Pharmaceutical Research, 2018; 8(1): 33-35.
12.    Wongrakpanich, S., et al., A comprehensive review of non-steroidal anti-inflammatory drug use in the elderly. Aging and disease, 2018; 9(1): 143.
13.    Suleyman, H., B. Demircan, and Y. Karagoz, Anti-inflammatory and side effects of cyclo-oxygenase inhibitors. Pharmacological reports, 2007; 59(3): 247.
14.    Bally, M., et al., Risk of acute myocardial infarction with NSAIDs in real world use: bayesian meta-analysis of individual patient data. bmj, 2017; 357: j1909.
15.    Vadnere, G., et al., Evaluation of HRBC Membrane Stabilization Activity of Cicer arietinum Linn. Pod Wall. Research Journal of Pharmacy and Technology, 2011; 4(9): 1465-1467.
16.    Haminiuk, C.W., et al., Phenolic compounds in fruits–an overview. International Journal of Food Science and Technology, 2012; 47(10): 2023-2044.
17.    Gutiérrez-Grijalva, E.P., 1. Review: dietary phenolic compounds, health benefits and bioaccessibility. Archivos Latinoamericanos de Nutrición, 2016; 66(2).
18.    Tungmunnithum, D., et al., Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 2018; 5(3): 93.
19.    AL-Azawi, A.H., W.Y. Salih, and Z.H. Hassan, Phytochemical and Antioxidant Activity of (Arachis hypogaea L.) Skin Extract Scultiv Atedin Iraq. Research Journal of Pharmacy and Technology, 2017; 10(11): 3721-3726.20.    
20.    Pourreza, N., Phenolic compounds as potential antioxidant. Jundishapur Journal of Natural Pharmaceutical Products, 2013. 8(4): p. 149.
21.    Watak, S. and S.S. Patil, Evaluation and Comparison of Antioxidant activity of herbomineral Complex. Research Journal of Pharmacognosy and Phytochemistry, 2012; 4(3): 171-177.
22.    Ambriz-Pérez, D.L., et al., Phenolic compounds: Natural alternative in inflammation treatment. A Review. Cogent Food and Agriculture, 2016; 2(1): 1131412.
23.    Macé, S., L. Truelstrup Hansen, and H. Rupasinghe, Anti-bacterial activity of phenolic compounds against Streptococcus pyogenes. Medicines, 2017; 4(2):  25.
24.    Chávez, J.H., et al., Evaluation of antiviral activity of phenolic compounds and derivatives against rabies virus. Veterinary microbiology, 2006; 116(1-3): 53-59.
25.    Basli, A., N. Belkacem, and I. Amrani, Health benefits of phenolic compounds against cancers. Phenolic compounds–Biological activity. London, UK: Intech Open, 2017; 193-210.
26.    Al Asaad, N. and D. Al Diab, Determination of total antioxidant activity of fruit juices widely consumed in Syria. Research Journal of Pharmacy and Technology, 2017; 10(4): 957-962.
27.    Frontela-Saseta, C., et al., Anti-inflammatory properties of fruit juices enriched with pine bark extract in an in vitro model of inflamed human intestinal epithelium: The effect of gastrointestinal digestion. Food and chemical toxicology, 2013; 53: 94-99.
28.    Dussossoy, E., et al. Anti-oxidative and anti-inflammatory effects of the Morinda Citrifolia fruit (noni). in III International Symposium on Human Health Effects of Fruits and Vegetables-FAVHEALTH 2009 1040. 2009.
29.    Vermerris, W. and R. Nicholson, Phenolic Compounds Biochemistry, Publisher Springer, New York. 2006.
30.    Sakat Sachin, S., N. Tupe Preeti, and R. Juvekar Archana, In-vitro anti-inflammatory activity of aqueous and methanol extracts of Erythrina indica Lam leaves. Pharmacologyonline, 2009; 3: 221-229.
31.    Gözlekçi, Ş., et al., Total phenolic distribution of juice, peel, and seed extracts of four pomegranate cultivars. Pharmacognosy Magazine, 2011; 7(26): 161.
32.    Anahita, A., R. Asmah, and O. Fauziah, Evaluation of total phenolic content, total antioxidant activity, and antioxidant vitamin composition of pomegranate seed and juice. International Food Research Journal, 2015; 22(3).
33.    Rapisarda, P., et al., Antioxidant effectiveness as influenced by phenolic content of fresh orange juices. Journal of Agricultural and Food Chemistry, 1999; 47(11): 4718-4723.
34.    Akhavan, H., et al., Phenolic compounds and antioxidant activity of juices from ten Iranian pomegranate cultivars depend on extraction. Journal of Chemistry, 2015. 2015.
35.    Chen, H., et al., Anti-inflammatory and antinociceptive properties of flavonoids from the fruits of black mulberry (Morus nigra L.). PloS one, 2016; 11(4).
36.    Manukumar, H. and K. Thribhuvan, In-vitro Evaluation of physicochemical, antioxidant and anti-inflammatory activity of pomegranate (Punica grantum L.) juice and seed hydro extracts. Int. J. Pharm. Bio. Sci, 2014; 5(1): 131-141.
37.    Ghanim, H., et al., Orange juice or fructose intake does not induce oxidative and inflammatory response. Diabetes Care, 2007; 30(6): 1406-1411.
38.    Oyekachukwu, A., et al., Anti-inflammatory effects of the chloroform extract of Annona muricata leaves on phospholipase A2 and prostaglandin synthase activities. Transl Biomed, 2017; 8(4): 137.
39.    Rajurkar, R., et al., Anti-inflammatory action of Abutilon indicum (L.) sweet leaves by HRBC membrane stabilization. Research Journal of Pharmacy and Technology, 2009; 2(2): 415-416.
40.    Shinde, U., et al., Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 1999; 70(3): 251-257.
41.    Chaudhuri, S., et al., Interaction of flavonoids with red blood cell membrane lipids and proteins: antioxidant and antihemolytic effects. International Journal of Biological Macromolecules, 2007; 41(1): 42-48.
42.    Asgary, S., G. Naderi, and N. Askari, Protective effect of flavonoids against red blood cell hemolysis by free radicals. Experimental and Clinical Cardiology, 2005; 10(2): 88.

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