Author(s): Riham Omar Bakr, Soumaya Saad Zaghloul, Reham Ibrahim Amer, Dalia Abd Elaty Mostafa, Mahitab Helmy El Bishbishy

Email(s): ,

DOI: 10.52711/0974-360X.2021.00633   

Address: Riham Omar Bakr1*, Soumaya Saad Zaghloul1, Reham Ibrahim Amer2,3, Dalia Abd Elaty Mostafa2, Mahitab Helmy El Bishbishy1
1Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787, Giza, Egypt.
2Pharmaceutics Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787, Giza, Egypt.
3Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021

Objective: Aegle marmelos (L.) Correa has been widely used in Indian traditional medicine and has many reported pharmacological activities. The aim of this research was to formulate solid lipid nanoparticles (SLNs) of Aegle oil (AO) that enhanced the beneficial antimicrobial activity of the oil. Methods: The chemical composition of Aegle leaf essential oil was analysed by GC-MS. Additionally, a phytochemical study of A. marmelos methanolic leaf extract was conducted using Folin-Ciocalteu colorimetric assay for determination of total phenolic content as well as ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-ESI-MS-MS) analyses for identification of individual components. Six formulations of AO-loaded SLNs (AO-SLNs) were prepared by a double emulsification method. The particle size, zeta potential (ZP), polydispersibility index (PDI) and drug encapsulation efficiency (EE) of the SLNs were determined. The morphology of the SLNs was observed by transmission electron microscopy (TEM). The antimicrobial activity of AO and AO-SLNs was assessed using disc diffusion method. Results: Thirty-two compounds were identified in the Aegle oil, of which ?-carene and a-phellandrene were the most abundant (48.14% and 34.14%, respectively). The estimated total phenolic content was 968mg gallic acid equivalents (GAE)/g, while UPLC-ESI-MS-MS led to the tentative characterization of thirteen metabolites. The SLNs showed ZP, PDI and EE 125 ± 0.22nm, –37.85, 0.282, and 92%, respectively. AO and AO-SLNs showed significant antimicrobial activity, and the SLNs could sustain the release of AO from their gel vehicles. Conclusion: Our results provide evidence for the application of AO-SLNs in topical and transdermal delivery systems.

Cite this article:
Riham Omar Bakr, Soumaya Saad Zaghloul, Reham Ibrahim Amer, Dalia Abd Elaty Mostafa, Mahitab Helmy El Bishbishy. Formulation, Characterization and Antimicrobial efficacy of Aegle marmelos Essential oil nanogel. Research Journal of Pharmacy and Technology. 2021; 14(7):3662-8. doi: 10.52711/0974-360X.2021.00633

Riham Omar Bakr, Soumaya Saad Zaghloul, Reham Ibrahim Amer, Dalia Abd Elaty Mostafa, Mahitab Helmy El Bishbishy. Formulation, Characterization and Antimicrobial efficacy of Aegle marmelos Essential oil nanogel. Research Journal of Pharmacy and Technology. 2021; 14(7):3662-8. doi: 10.52711/0974-360X.2021.00633   Available on:

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