The immune system plays a role in protective mechanism against the foreign and its modulation either suppression or stimulation was implicated in several human diseases like infection, inflammatory and autoimmune diseases, etc. For which several allopathic immunomodulators have been used for treatment, exhibit potential side effects. Biological active compounds from natural sources used in the treatment of an immune-related disorder. Being the importance of natural compound. The present study was undertaken to evaluate the immunomodulatory activity of methanolic roots extract of Pavonia odorata and isolated compounds from Pavonia odorata. Immunomodulatory activities were determined by in vivo models- delayed-type hypersensitivity (DTH), carbon clearance assay (Phagocytic index), and cyclophosphamide-induced immunosuppression. The methanolic extracts of Pavonia odorata and isolated compound, in the concentration range 5, 10, 25, 50 and 100µg were also tested for phagocytic effect on neutrophils using the in vitro models-nitroblue tetrazolium (NBT) dye test. Mice treated with dose i.e. 200mg/kg (Group III) and dose 370mg/kg (Group-IV) increase response in footpad edema was found to be statistically significant (*p? 0.05), (**p? 0.01) and showed statistically significant (*p? 0.05) increase in WBC and platelets count and phagocytic index when compared to cyclophosphamide (20mg/kg) treated and control-treated mice. In cyclophosphamide induces immunosuppression, methanolic roots extract Pavonia odorata and isolated compound showed significant (P < 0.001) increase total differential leucocytes count DLC and hematological parameters RBCs, Hb, Platelets and WBCs significant increases when compared with cyclophosphamide group. Methanolic extract of Pavonia odorata and isolated compound in the concentration range 5, 10, 25, 50 and 100µg also showed significant (p? 0.001) phagocytic effect on neutrophils in the parameter studied. The present experimental finding demonstrated that isolated compound has superior immunomodulatory activity then methnolic extract of Pavonia odorata this effect presumably due to the greater ability of isolated compound to boost the innate and adaptive immune system.
Cite this article:
Shantilal Singune, Jai S Vaghela. Investigation of Immunomodulatory Activity of Methanolic Extract and Isolated Compound of Pavonia odorata Roots in Mice. Research Journal of Pharmacy and Technology. 2021; 14(7):3489-4. doi: 10.52711/0974-360X.2021.00605
Shantilal Singune, Jai S Vaghela. Investigation of Immunomodulatory Activity of Methanolic Extract and Isolated Compound of Pavonia odorata Roots in Mice. Research Journal of Pharmacy and Technology. 2021; 14(7):3489-4. doi: 10.52711/0974-360X.2021.00605 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-2
1. R. Sathish, P. Sravan Kumar, K. Natarajan, N. Sridhar. Hepatoprotective and Anti Pyretic Activities of Methanolic Extract of Butea Monosperma Lam Stem Bark In Wister Rats Asian journal of Pharmaceutical Research Asian J. Pharm. Res. 1(4): Oct. - Dec. 2011; Page 130-133.
2. Vivekanand Katare, Chandra Kishore Tyagi Phytochemical and Pharmacological Evaluation of Martynia annua for Immunomodulatory Potential Res. J. Pharmacognosy and Phytochem. 2020; 12(2): 94-100.
3. Anitha Kumari, K.G. Lalitha, T. Venkatachalam, P. Kalaiselvi, M.G. Sethuraman. Anti-inflammatory and Antinociceptive activity of Pavonia zeylanica Linn Asian journal of research in Pharmaceutical sciences 2011 Asian J. Res. Pharm. Sci. 1(4): Oct.-Dec. 2011; Page 113-116.
4. Madan Singh, Prashant Soni, Neeraj Upmanyu and Yogesh Shivhare. In-vitro Anti-arthritic Activity of Manilkara zapota Linn Asian J. Pharm. Tech. 1(4): Oct. - Dec. 2011; Page 123-124.
5. Rajesh B, Ramasubbualakshmi A, Gopalasathees Kumar K, Bharathi D, Nishanth R Phytochemical Analysis and Anti-arthritic Activity of Ficus carica Leaves Asian journal of research in Chemistry Asian J. Research Chem. 2020; 13(2): 151-154.
6. Ukpo G. Ogbonnia S. Ehianeta T. Bashir S. Immunostimulatory and biochemical effects of ethanolic extract of Mangifera indica stem bark on dexamethasone-induced immunosuppressed male rats. Int J Pharm Pharm Sci, 2013, 5(2): 569-572.
7. Gayathiri. K, Gopi Sudheer Kumar. J, Kavimani. Evaluation of Immunomodulatory activity of Salvia hispanica. L Seeds Research J. Pharm. and Tech 2017; 10(12): 4255-4260.
8. Palaksha M. N, Ravishankar K, Vijaya Nandini. Evaluation of the Immunomodulatory Effect of Eclipta prostrata whole Plant Extract on Albino Rats Res. J. Pharmacology and Pharmacodynamics. 2017; 9(2): 41-45.
9. DS Hugar, Sanjeev Heroor and Zakir Hussain. Screening of Immunomodulatory Activity of Extracts of Seeds of Jatropha curcas Linn. On Human Neutrophils Research Journal of Pharmacognosy and Phytochemistry. 2(1): Jan.-Feb. 2010, 70-74.
10. Kuttan G. Immunomodulatory effect of come naturally occurring sulfur-containing compounds. Journal of Ethnopharmacol. 2000, 72: 93-99.
11. Bodhankar S, Makare N, Rangari V. Immunomodulatory activity of alcoholic extract of Mangifera indica L. in mice. J Ethnopharmacol. 2001, 78: 133-137.
12. Pezzuto JM. Plant-derived anticancer agents. Biochemical Pharmacology. 1997, 53: 121–133.
13. Diasio RB, LoBuglio AF. The Pharmacological Basis of Therapeutics. In Goodman, Gilman’s, editors, McGraw-Hill (NY), 1996, 12: 1291–1307.
14. Patil US. Jaydeokar V. Bandawane DD. Immunomodulators: A Pharmacological Review. Int J Pharm Pharm Sci, 2012, 4(1): 30-36.
15. The wealth of India- Raw material, Vol II. N-Pe, Information and Publication Directorate, CSIR, New Delhi, 1992.
16. Thamil Selvan V, Kakoti BB, Gomathi P, Ashok Kumar D, Aminul Islam, Gupta M, Mazumder UK. Cytotoxic and antitumor activities of Pavonia odorata against Ehrlich ascites carcinoma cells bearing mice. Pharmacologyonline, 2007; 2: 453-477.
17. Srikumar R., Jeya P.N., Manikandan S., Sathya N.G. and D.R. Shella. Effect of Triphala on oxidative stress and cell-mediated immune response against noise stress in rats. Mol. Cell Biochem. 2006; 283: 67-74.
18. OECD: Guideline, 423, acute oral toxicity: Environmental Health and Safety Monograph series on Testing and Assessment No. 24, 2000.
19. Satish Verma K, Asima Shaban, Reena Purohit, Madhvi Chimata L, Geeta Rai, and Om Prakash Verma. Immunomodulatory activity of Withania somnifera (L.). Journal of Chemical and Pharmaceutical Research. 2012; 4(1): 559-561.
20. Ghule B, Murugananthan G, Nakhate P, Yeole P. Immunositmulant effect of Capparis zeylanica Linn. Leaves. J Ethnopharmacol. 2006, 108: 311-315.
21. Hudson L, Hay FC. Practical immunology. (Blackwell. London. 1980, 73-92.
22. Ziauddin, M., N. Phansalkar, P. Patki, S Diwanay, and B Patwardhan, 1996 on Studies on the Immunomodulatory effects of ashwagandha J. Ethnopharmacol.,50: 69-76.
23. Vellaythum Ravichandrian and Patil Vishal S Invitro evaluation of immunomodulatory of Acorus calamus on human Neutrophils International Research Journal of Pharmacy, 2015 6 (7), 450-452.
24. Delvas TJ, Roitt IM, Encyclopedia of immunology 2nd ed London, Academic Press 1998 198-23.
25. Mitra SK, Guptamandsarma DNK, the immunomodulatory effect of IM 133 Phytother Res 1999 13 341-3.
26. Geo-Vigila A and Baskaran X: Immunomodulatory effect of coconut protein on cyclophosphamide-induced immune-suppressed swiss albino mice. Ethnobotanical Leaflets 2008; 12: 1206-12.