Author(s): Atul S. Sayare, Priti B. Undre, Prashant D. Ghode, Sujata V. Singh, Shweta P. Ghode

Email(s): atulsayare@gmail.com

DOI: 10.52711/0974-360X.2021.00604   

Address: Atul S. Sayare1*, Priti B. Undre1, Prashant D. Ghode1, Sujata V. Singh1, Shweta P. Ghode2
1Department of Pharmaceutical Quality Assurance, JSPM’s Rajarshi Shahu College of Pharmacy and Research, Pune, Maharashtra, India – 411033.
2Rasiklal M. Dhariwal Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India – 411019.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021


ABSTRACT:
A simple, specific, precise, and accurate RP-HPLC method has been developed and validated for the estimation of Clomiphene citrate in bulk and pharmaceutical dosage form using C18 column Shimadzu (250mm × 4.5mm × 5µm) with a mobile phase consisting of 900mL of HPLC grade methanol and 100mL of HPLC grade acetonitrile. The mobile phase was sonicated for 10 min and filtered through a 0.45µm membrane filter at a flow rate of 1.0mL/min. The detection was carried out at 295nm and retention time of Clomiphene citrate was found to be 3.44 min. Linearity was observed in the concentration range of 10–50µg/mL (coefficient of determination R2=0.999) with regression equation y =20321x + 60021. The method was validated as per ICH guidlines.


Cite this article:
Atul S. Sayare, Priti B. Undre, Prashant D. Ghode, Sujata V. Singh, Shweta P. Ghode. Development and Validation of RP-HPLC Method for the Estimation of Clomiphene Citrate in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2021; 14(7):3483-8. doi: 10.52711/0974-360X.2021.00604

Cite(Electronic):
Atul S. Sayare, Priti B. Undre, Prashant D. Ghode, Sujata V. Singh, Shweta P. Ghode. Development and Validation of RP-HPLC Method for the Estimation of Clomiphene Citrate in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2021; 14(7):3483-8. doi: 10.52711/0974-360X.2021.00604   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-7-1


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