Author(s):
Jigyasa Pathak, Aswathi M. P., B. R. Patel, Harisha C. R., Shukla Vinay J.
Email(s):
jigyasapathak30@gmail.com
DOI:
10.52711/0974-360X.2021.00517 
Address:
Jigyasa Pathak1, Aswathi M. P.2, B. R. Patel3, Harisha C. R.4, Shukla Vinay J.5
1,2Ph. D. Scholar, Dept. of Dravyaguna, IPGT & RA, Jamnagar.
3Associate Professor, Dept. of Dravyaguna, IPGT & RA, Gujarat Ayurved University, Jamnagar.
4Head, Pharmacognosy Laboratory, IPGT & RA, Jamnagar.
5Head, Pharmaceutical Chemistry Laboratory, IPGT & RA, Jamnagar.
*Corresponding Author
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Background: Desmodium velutinum (Fabaceae) is one of the wild edible plants used traditionally to treat diarrhoea, haematuria, infertility and also externally used to treat scorpion bites. Though various parts of the plant have been used for therapeutic purpose, panchanga of the plant has not been studied for its Pharmacognostical and phytochemical characteristics in detail, following scientific parameters. Hence in the present study Desmodium velutinum whole plant has been studied for its pharmacognostical and phytochemical characters and compared with commonly used species Desmodium gangeticum. Methods: Whole plant of Desmodim velutinum was collected from Paikmal, Odisha and Desmodium gangeticum was collected from Junagarh, Gujarat in the month of November 2019 following good collection practices and shade dried. Pharmacognostical study and analytical study were performed following standard pharmacopoeial procedures. Results: Whole plant (Panchanga) of former is sweetish and latter is bitter. Powder microscopy of Desmodium gangeticum showed presence of rhomboidal crystals and lignified annular and spiral vessels. Desmodium velutinum showed presence of prismatic crystals and lignified pitted vessels. Physico-chemical analysis showed higher loss on drying in Desmodium gangeticum, and higher water soluble extractive values of Desmodium velutinum. Phytochemical estimation showed the presence of Proteins, tannins, and carbohydrates in both samples. HPTLC study showed similar Rf value 0.82 and 0.94 at 254 and 0.03 and 0.41 at 366nm. Conclusion: Basing upon the analytical and microscopic similarities Desmodium velutinum may be considered as substitute of Desmodium gangeticum.
Cite this article:
Jigyasa Pathak, Aswathi M. P., B. R. Patel, Harisha C. R., Shukla Vinay J. Microscopic and Phytochemical analysis of Desmodium velutinum (Willd) DC and Desmodium gangeticum (L.) DC. Panchanga powder. Research Journal of Pharmacy and Technology. 2021; 14(6):2950-6. doi: 10.52711/0974-360X.2021.00517
Cite(Electronic):
Jigyasa Pathak, Aswathi M. P., B. R. Patel, Harisha C. R., Shukla Vinay J. Microscopic and Phytochemical analysis of Desmodium velutinum (Willd) DC and Desmodium gangeticum (L.) DC. Panchanga powder. Research Journal of Pharmacy and Technology. 2021; 14(6):2950-6. doi: 10.52711/0974-360X.2021.00517 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-7
REFERENCE:
1. H.O. Saxena, H.O. Brahma-The Flora of Orissa. Vol 1. April 1994. Regional Research Laboratory and Orissa Forest Division Corporation. 499, 514
2. Ezike AC, P.A. Akah, C.O. Okoli, I.K. Ufere, E. Ezeudu CFO, C. Ashara, I.N. Igbokwe. Studies on Gastrointestinal effects of Desmodium velutinum: A traditional remedy for Diarrhea. American Journal of Pharmacology and Toxicology. 2014;9(2):114 - 24.
3. Basant Lal Gupta. Forest Flora of the Chakrata, Dehradun and Saharanpur forest divisions, Uttar Pradesh. 3rd edition. Delhi: The manager of Publications ;1969. Pg 184
4. Mohan Aswathi, Pathak Jigyasa, Patel B.R. An appraisal on ethnomedicinal claims and Pharmacological activities of Desmodium velutinum (Willd.) D.C. International Journal of Pharmaceutical Research 2020;12(2). 622-30
5. Guha Bakshi D.N. Sensarma P. A lexicon of medicinal plants in India Vol 2. Calcutta: Naya Prokash;, Sep 2001.Pg 48,49
6. Singh Suman, Parmar Neha, Patel B.R. A review on Shalaparni (Desmodium gangeticum DC.) and Desmodium species (Desmodium triflorum DC. and Desmodium laxiflorum DC)-Ethnomedicinal perspectives. Journal of Medicinal Plant Studies 2015; 3(4): 38-43
7. Trease and Evans WC. Pharmacognosy. 16th ed. London: Saunders Elsevier; 2009. 567-68.
8. Khandelwal KR. Practical Pharmacognosy. 19th ed. Pune: Nirali Prakashan; 2008.
9. Anonymous. The Ayurvedic Pharmacopoeia of India. Part-I, Vol-III. 1st ed. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of AYUSH; 2008.
10. Baxi AJ, Shukla VJ, Bhatt UB. Method of Qualitative Testing of some ayurvedic Formulations. Jamnagar: Gujarat Ayurved University; 2001. 5‑12.
11. Ravishankar S. Textbook of Pharmaceutical Analysis. 4th ed. Pune: Rx Publication; 2010.
12. Egon S. Thin Layer Chromatography. Springer Publication,1969, Reprint 2005.
13. Hemlal H, Subban Ravi. GC-MS, HPTLC and Antimicrobial analysis of Root extracts of Pseudarthria viscida and Arn and Desmodium gangeticum (Linn) DC. International Research Journal of Biological Sciences 2012;1(5): 57-65
14. Anonymous. The Ayurvedic Pharmacopoeia of India. Part-I, Vol-VII. 1st ed. New Delhi: Government of India, Ministry of Health and Family Welfare, Department of AYUSH; 2008
15. Lakhmale Sarang, Acharya Rabinarayan, Gorai Dilip, Roy Rajiv. Phytochemical analysis of Symphorema Polyandrum Wight.seeds by GCMS. Indo American Journal of Pharmaceutical Research 2107; 7(05): 8852-62.
16. World Health Organization. Quality Control Methods for Medicinal Plant Materials. Geneva: World Health Organization; 1998.31.
17. Priyadarshi A, Kumari R, Sharma AK, Jaiswal ML. Preliminary pharmacognostic and phytochemical investigation of Blepharis indica-T Anders seeds. Anc Sci Life 2016; 36:78-83
18. Chauhan Ashish, Goyal Manish. GCMS technique and its analytical applications in Science and Technology. Journal of Analytical and Bioanalytical Techniques 2014; 5(6):1-5
19. Pathak Jigyasa, Patel B.R. Evaluation of Desmodium velutinum (WILLD.) DC as a substitute of Shalaparni-Desmodium gangeticum (L.) DC. in the management of Kasa: A Pharmacotherapeutical study, PhD thesis at IPGTRA Gujarat Ayurved University 2018