Author(s): Nozim N. Khoshimov, Guli M. Raimova, Kabul E. Nasirov, Zulayho A. Mamatova, Nodira I. Mamadaliyeva, Abbaskhan S. Тuraev

Email(s): khoshimovn@inbox.ru

DOI: 10.52711/0974-360X.2021.00571   

Address: Nozim N. Khoshimov1*, Guli M. Raimova1, Kabul E. Nasirov1, Zulayho A. Mamatova2, Nodira I. Mamadaliyeva3, Abbaskhan S. Тuraev4
1Institute of Biophysics and Biochemistry at the National University of Uzbekistan. 100174, Tashkent City, Almazar District, Students Town, University St., 174.
2National University of Uzbekistan. Tashkent, Uzbekistan. 100174, Tashkent City, Almazar District, Students Town, University St., 174.
3Tashkent State Pedagogical University Named After Nizami. 100070, Tashkent City, Chilanzor District, Bunyodkor Ave., 27.
4The Institute of Bioorganic Chemistry Named After A.S. Sadikov. 100125, Tashkent City, Mirzo Ulugbek District, Mirzo Ulugbek Avenue, 83
*Corresponding Author

Published In:   Volume - 14,      Issue - 6,     Year - 2021


ABSTRACT:
Experimental studies and analyses of new compounds with different mechanisms of action on systemic haemostasis are relevant for the identification and development of potential pharmacological preparations. The modified sulphated polysaccharides with anticoagulant and antithrombin activity were studied for haemostasis. Platelet-rich plasma was obtained by centrifugation at 200g for 10 minutes. The remaining citrate blood was further centrifuged at 1500g for 10 min to obtain platelet-poor plasma. The antithrombin activity of the compounds was evaluated In vitro by their effect on the recalcification time, thrombin and prothrombin time of rabbit and human blood plasma stabilized with a 3.8% sodium citrate solution in the ratio 9:1. The results showed that the anticoagulant activity of the studied sulphates increased with an increasing degree of sulphation. Sulphated polysaccharides showed strong anticoagulant activity In vitro. The experimental results showed a significant increase in the coagulation time of blood plasma in tests for prothrombin and thrombin time. These properties of these components are of particular interest, and further detailed studies of the physicochemical characteristics and mechanisms of action of these molecules should be performed, which will eventually allow them to be used as heparin-like drugs.


Cite this article:
Nozim N. Khoshimov, Guli M. Raimova, Kabul E. Nasirov, Zulayho A. Mamatova, Nodira I. Mamadaliyeva, Abbaskhan S. Тuraev. The effect of Sulphated cellulose on System of Haemostasis. Research Journal of Pharmacy and Technology. 2021; 14(6):3283-9. doi: 10.52711/0974-360X.2021.00571

Cite(Electronic):
Nozim N. Khoshimov, Guli M. Raimova, Kabul E. Nasirov, Zulayho A. Mamatova, Nodira I. Mamadaliyeva, Abbaskhan S. Тuraev. The effect of Sulphated cellulose on System of Haemostasis. Research Journal of Pharmacy and Technology. 2021; 14(6):3283-9. doi: 10.52711/0974-360X.2021.00571   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-61


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