Author(s): Kailash Chander Malik, Manoj Karwa, Gaurav K. Jain, Rohit Dutt

Email(s): rohitdatt23@rediffmail.com

DOI: 10.52711/0974-360X.2021.00563   

Address: Kailash Chander Malik1, Manoj Karwa2, Gaurav K. Jain3, Rohit Dutt1
1Department of Pharmacy, School of Medical and Allied Sciences, G D Goenka University, Gurugram - 122103, India.
2Auriga Research Private Limited, Plot No. 136, Sector - 5, IMT Manesar, Gurugram.
3Department of Pharmaceutics, Delhi Pharmaceutical Sciences and Research University (DPSRU), Govt. of NCT of Delhi, New Delhi, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 6,     Year - 2021


ABSTRACT:
A simple, sensitive, and selective ultra-performance liquid chromatographic (UPLC) method with mass spectrometric (UPLC-MS/MS) detection was developed for the determination of Rivaroxaban in human plasma sample. After a simple protein-precipitation by acetonitrile, the post-treatment samples were separated on a UPLC Bridged Ethyl Hybrid (BEH) C-18 column with 0.2% (v/v) formic acid in water: acetonitrile (60:40, v/v) as a mobile phase and analyzed in positive ion mode. The method developed was validated in human plasma with a daily working range of 2.02–997.72ng/mL with correlation coefficient, r2=0.999936. The method proved to be accurate (recovery, 97.0–107.95%), precise (2.21-7.21%), rapid (run time 2.5 min) and specific. In conclusion, the present validated method could be applied for pharmacokinetic study and bioequivalence testing of Rivaroxaban products.


Cite this article:
Kailash Chander Malik, Manoj Karwa, Gaurav K. Jain, Rohit Dutt. Development and Validation of UPLC-MS/MS Method for the determination of Rivaroxaban in human plasma using Liquid-liquid extraction. Research Journal of Pharmacy and Technology. 2021; 14(6):3239-3. doi: 10.52711/0974-360X.2021.00563

Cite(Electronic):
Kailash Chander Malik, Manoj Karwa, Gaurav K. Jain, Rohit Dutt. Development and Validation of UPLC-MS/MS Method for the determination of Rivaroxaban in human plasma using Liquid-liquid extraction. Research Journal of Pharmacy and Technology. 2021; 14(6):3239-3. doi: 10.52711/0974-360X.2021.00563   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-53


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