Author(s):
Sofi N Stiani, Anas Subarnas, Taofik Rusdiana
Email(s):
sofia240586@gmail.com
DOI:
10.52711/0974-360X.2021.00514 
Address:
Sofi N Stiani1,2, Anas Subarnas3, Taofik Rusdiana1
1Department of Pharmaceutics and Pharmaceutical Technology, Padjadjaran University, Indonesia.
2Pharmacy Study Program, STIKes Salsabila, Serang Banten, Indonesia.
3Department of Pharmacology and Clinical Pharmacy, Padjadjaran University, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Apigenin is a flavonoid belonging to a flavone subclass that is reported to have many benefits and less toxic than other flavonoid groups. Objective: The purpose of this study is to evaluate antinephrolithiasis activity of modified Hot Melt Extrusion (HME) apigenin formulas with Soluplus® and Kollidon® VA 64 polymers in rats. Method: In this study, rats were administered ethylene glycol 0.75% and ammonium chloride 2% as inducing agents for formation of kidney stone. Rats in each group were given the formulas of apigenin without HME, or apigenin with HME soluplus® polymer and apigenin with HME Kollidon® VA 64 polymer every day for seven days. The concentration of calcium oxalate in the kidney and the urine was measured using the atomic absorption spectrophotometer (AAS). Urine volume and urine pH were also measured. Result: The results of this study indicated that the apigenin formulas with HME soluplus® polymers can shed kidney stones at a dose of 4.8mg/kg of body weight. Its effect was stronger than the apigenin formula with Kollidon®. Conclusion: The formula of Apigenin with HME Soluplus® polymers is better than that of Kollidon® polymers in removing kidney stones with the ratio of 10:90 at a dose of 4.8mg/kg.
Cite this article:
Sofi N Stiani, Anas Subarnas, Taofik Rusdiana. Anticalculi Activities of Apigenin Hot Melt Extrusion Results with Soluplus® and Kollidon® VA 64 Polymers on Wistar Rats. Research Journal of Pharmacy and Technology. 2021; 14(6):2931-6. doi: 10.52711/0974-360X.2021.00514
Cite(Electronic):
Sofi N Stiani, Anas Subarnas, Taofik Rusdiana. Anticalculi Activities of Apigenin Hot Melt Extrusion Results with Soluplus® and Kollidon® VA 64 Polymers on Wistar Rats. Research Journal of Pharmacy and Technology. 2021; 14(6):2931-6. doi: 10.52711/0974-360X.2021.00514 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-4
REFERENCES:
1. Scales CD, Smith AC, Hanley JM, Saigal CS. Prevalence of kidney stones in the United States. Eur Urol. 2012. doi:10.1016/j.eururo.2012.03.052
2. Li WM, Chou YH, Li CC, et al. Association of body mass index and urine pH in patients with urolithiasis. Urol Res. 2009. doi:10.1007/s00240-009-0194-4
3. Frassetto L, Kohlstadt I. Treatment and prevention of kidney stones: An Update. Am Fam Physician. 2011.
4. Kalai R, Sasirekha B. Dietary Guidelines to Reduce the Risk of Renal Stones. Int J Nurs Educ Res. 2018. doi:10.5958/2454-2660.2018.00018.2
5. Mayans L. Nephrolithiasis. Prim Care - Clin Off Pract. 2019. doi:10.1016/j.pop.2019.02.001
6. Dimas Nugroho, Ponco Birowo NR. Percutaneous Nephrolithotomy Sebagai Terapi Batu Ginjal. Maj Kedokt Indones.: 2011.
7. Jagtap PN, Vyapari BR, Nimbalkar YH, Kale S V., Nigade GB. Evaluation of antiurolithiatic activity of polyherbal formulation (Lithout tablets) by In-vitro inhibition of calcium oxalate crystallization. Res J Pharm Technol. 2019. doi:10.5958/0974-360X.2019.00950.8
8. Kushagra D, Rajiv S, Ruchi G, Neelesh M. In-vitro evaluation of anti-urolithic activity of leaves extract of costus igneus. Res J Pharm Technol. 2020. doi:10.5958/0974-360X.2020.00237.1
9. Kachru A, Bisht M, Baunthiyal M. In vitro evaluation of anti-neprolithiatic activity of leaves and seeds of Macrotyloma uniflorum on dissolution or removal of kidney stones. Res J Pharmacogn Phytochem. 2016. doi:10.5958/0975-4385.2016.00002.9
10. Praveena A, Sanjayan KP. Pharmacognostic Studies on Root-bark and fruit of Morinda tinctoria Roxb. Res J Pharmacogn Phytochem. 2018. doi:10.5958/0975-4385.2018.00034.1
11. Chandaka Madhu, L. Harish, K. Narsimha Reddy, S. Sagar, D. Soumya GPK. Antiurolithic Activity of Aqueous Extract on Roots and Seeds of Plectranthus tomentora on Ethylene Glycol Induced kidney Stones in Male Albino Rats. Asian J Res Pharm Sci. 2012;2(4): 129-133.
12. Tiwari R V., Patil H, Repka MA. Contribution of hot-melt extrusion technology to advance drug delivery in the 21st century. Expert Opin Drug Deliv. 2016. doi:10.1517/17425247.2016.1126246
13. Chokshi R, Zia H. Hot-Melt Extrusion technique: A Review. Iran J Pharm Res. 2004;3(December 2003):3-16. doi:10.1016/j.ijpharm.2016.04.057
14. Kumar AMS, Kulyadi GP, Mutalik S, Kulkarni VI, Tippavajhala VK. Application of hot melt extrusion for the solubility enhancement of a BCS class II drug. Res J Pharm Technol. 2019. doi:10.5958/0974-360X.2019.00642.5
15. Kolter, Karl and Karl M. Suitability of Plasticized Polymers for Hot Melt Extrusion. Exipients & Actives for Pharma. 2010; No.24:2-6. www.pharma-ingredients.basf.com.
16. Daisy Sharma, Mohit Soni, Sandeep Kumar GG. Solubility Enhancement – Eminent Role in Poorly Soluble Drugs. Res J Pharm Tech. 2009; 2(2): 220-224.
17. Agrawal A, Dudhedia M, Deng W, et al. Development of Tablet Formulation of Amorphous Solid Dispersions Prepared by Hot Melt Extrusion Using Quality by Design Approach. AAPS Pharm Sci Tech. 2016;17(1):214-232. doi:10.1208/s12249-015-0472-0
18. Shetty R, Tallapaneni V, M SR, et al. Solubility Enhancement of Lumefantrine by Hot Melt Extrusion Process. Res J Pharm Technol. 2019. doi:10.5958/0974-360x.2019.00493.1
19. Kayalvizhi D, Sivakumar V, Jayanthi M. Phytochemical screening and antinephrolithiasis activity of ethanol extract of Aerva lanata on ethylene glycol induced renal stone in rats. Res J Pharm Technol. 2015;8(11):1481-1486. doi:10.5958/0974-360X.2015.00265.6
20. Mentari, aisyah et al. Antinephrolithiasis effect of ethanol extract of Phaleria macrocarpa (Scheff.) boerl in male wistar rat. J Innov Pharm Biol Sci. 2018;5(1): 42-45.
21. Srinivas V, Sumalatha G, Jagannath P, Kishore D, Kiran P, Rani M. Antinephrolithiatic Potential of Macrotyloma uniflorum Aqueous Extract in Rats. Res J Pharmacogn Phytochem. 2010.
22. Wagner CA, Mohebbi N. Urinary pH and stone formation. J Nephrol. 2010. doi:10.5167/uzh-45805
23. Cruzan G, Corley RA, Hard GC, et al. Subchronic toxicity of ethylene glycol in Wistar and F-344 rats related to metabolism and clearance of metabolites. Toxicol Sci. 2004;81(2): 502-511. doi:10.1093/toxsci/kfh206
24. Atmani F, Slimani Y, Mimouni M, Hacht B. Prophylaxis of calcium oxalate stones by Herniaria hirsuta on experimentally induced nephrolithiasis in rats. BJU Int. 2003. doi:10.1046/j.1464-410X.2003.04289.x