Author(s): Heba R. Mohamed, El-Sayed S. Abdel-Hameed, Eman A. El-Wakil, Maher M. El-Hashash, Mohamed Shemis

Email(s): hebaraafat_p@sci.asu.edu.eg

DOI: 10.52711/0974-360X.2021.00544   

Address: Heba R. Mohamed1*, El-Sayed S. Abdel-Hameed1, Eman A. El-Wakil1, Maher M. El-Hashash2, Mohamed Shemis3
1Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Korniash El-Nile, 12411Warrak El-Hadar, Giza, Egypt.
2Department of Chemistry, Faculty of Science, Ain-Shams University, El-Khalifa El-Mamoun, 11566 Abasia, Cairo, Egypt.
3Laboratory of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Korniash El-Nile, 12411Warrak El-Hadar, Giza, Egypt.
*Corresponding Author

Published In:   Volume - 14,      Issue - 6,     Year - 2021


ABSTRACT:
Few studies had been conducted on the phytochemical profile and the biological activities of Brachychiton rupestris. The antioxidant activity of 85% methanolic extract of B. rupestris leaves and its derived fractions was assessed using five different in-vitro assays which are DPPH• assay, phosphomolybdenum assay (TAC), hydroxyl radical (•OH) scavenging assay, nitric oxide radical (NO•) scavenging assay and permanganate reducing antioxidant capacity (PRAC). The total phenolic, flavonoid and flavonol contents were also determined for the different plant samples using Folin-Ciocalteu, aluminum chloride and aluminum chloride/ sodium acetate methods, respectively. In-vitro cytotoxic activity of the crude extract and its fractions against HepG2 cell line was also evaluated via SRB assay. The ethyl acetate fraction obtained from the aqueous methanolic extract of the plant leaves possessed the most potent antioxidant potential according to the different applied assays (DPPH• SC50 = 25.50±0.76 µg/mL, TAC= 311.98±1.19 mg AAE/g ext., •OH SA= 68.42±0.15%, NO• SA= 33.68±1.98% & PRAC = 62.88±0.27%). It also owed the highest amount of phenolics (TPC= 342.10±2.08 mg GAE/ g ext.) whose majority were flavonoids (TFC= 309.82±3.64 mg QE/ g ext.). A strong positive correlation was found between the total phenolic contents of various plant samples and their antioxidant activity. The butanol derived fraction exhibited the most potent cytotoxic activity (IC50 = 8.60 µg/mL). To the best of our knowledge, it is the first study reporting the antioxidant and the cytotoxic activities of B. rupestris leaves. The study revealed that the B. rupestris leaves could be a good source of natural antioxidants which may be applied in food and pharmaceutical industries. They could also be a new opportunity of discovering anti-mutagenic agents.


Cite this article:
Heba R. Mohamed, El-Sayed S. Abdel-Hameed, Eman A. El-Wakil, Maher M. El-Hashash, Mohamed Shemis. Phytochemical Screening, In-vitro Antioxidant and Cytotoxic potentials of Brachychiton rupestris Leaves. Research Journal of Pharmacy and Technology. 2021; 14(6):3119-7. doi: 10.52711/0974-360X.2021.00544

Cite(Electronic):
Heba R. Mohamed, El-Sayed S. Abdel-Hameed, Eman A. El-Wakil, Maher M. El-Hashash, Mohamed Shemis. Phytochemical Screening, In-vitro Antioxidant and Cytotoxic potentials of Brachychiton rupestris Leaves. Research Journal of Pharmacy and Technology. 2021; 14(6):3119-7. doi: 10.52711/0974-360X.2021.00544   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-34


REFERENCES:
1.    Chigayo K, Mojapelo PEL, Mnyakeni-moleele S, Misihairabgwi JM. Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers. Asian Pacific Journal of Tropical Biomedicine. 2016; 6(12): 1037-1043.
2.    Yousif F, Hifnawy MS, Soliman G, Boulos L, Labib T, Mahmoud S, Ramzy F, Yousif M, Hassan I, Mahmoud K, El-Hallouty SM, El-Gendy M, Gohar L, El-Manawaty M, Fayyad W, El-Menshawi BS. Large-scale in vitro Screening of Egyptian Native and Cultivated Plants for Schistosomicidal Activity. Pharmaceutical Biology. 2007; 45(6): 501-510.
3.    Thabet AA, Youssef FS, El-shazly M, Singab AB. Anti-infective Properties of Brachychiton rupestris and Brachychiton luridum Leaves and their Qualitative Phytochemical Screening. Journal of Medicinal and Aromatic Plants. 2017; 6(4): 4-11.
4.    Mpala L, Chikowe G, Cock IE. Brachychiton rupestris (T.Mitch. ex Lindl.) K. Schum. Extracts Inhibit the Growth of Streptococcus pyogenes. Pharmacognosy Communications. 2019; 9(1): 27-33.
5.    Thabet AA, Youssef FS, El-shazly M, El-beshbishy HA, Singab AB. Validation of the antihyperglycaemic and hepatoprotective activity of the flavonoid rich fraction of Brachychiton rupestris using in vivo experimental models and molecular modelling. Food and Chemical Toxicology. 2018; 114: 302-310.
6.    Thabet AA, Youssef FS, El-Shazly M, Singab AB. GC-MS and GC-FID analyses of the volatile constituents of Brachychiton rupestris and Brachychiton discolor, their biological activities and their differentiation using multivariate data analysis. Natural Product Research. 2018; 34(4): 590-594.
7.    El-Sherei MM, Ragheb AY, Kassem ME, Marzouk MM, Mosharrafa SA, Saleh NA. Phytochemistry, biological activities and economical uses of the genus Sterculia and the related genera: A reveiw. Asian Pacific Journal of Tropical Disease. 2016; 6(6): 492-501.
8.    Chikara S, Nagaprashantha LD, Singhal J, Horne D, Awasthi S, Singhal SS. Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment. Cancer Letters. 2018; 413: 122-134.
9.    Sowjanya K, Manjula RR, Vaishnavi V, Sai BK, Divya K. In-Vivo Antioxidant Activity of Hibiscus plantifolius Stems. Research Journal of Pharmacy and Technology. 2019; 12(6): 2712-2715.
10.    Parimi R, Pravallika KE. Studies on Phytochemical Screening, Total Phenolic Content and In vitro Antioxidant activity of Different Extracts of Euphorbia thymifolia Roots. Research Journal of Pharmacy and Technology. 2017; 10(2): 551-556.
11.    Jadhav GB, Saudagar RB. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian Journal of Research in Pharmaceutical Sciences. 2014; 4(2): 51-54.
12.    Kumar H, Savaliya M, Biswas S, Nayak PG, Maliyakkal N, Setty MM, Gourishetti K, Pai KSR. Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract / fractions of Mimusops elengi Linn. Cytotechnology. 2016; 68(4): 861-877.
13.    Omar A, Abou-Alfa GK, Khairy A, Omar H. Risk factors for developing hepatocellular carcinoma in Egypt. Chinese Clinical Oncology. 2013; 2(4): 1-9.
14.    Bera TK, Chatterjee K, Ghosh D. In-vitro antioxidant properties of the hydro-methanol extract of the seeds of Swietenia mahagoni (L.) Jacq. Biomarkers and Genomic Medicine. 2015; 7(1): 18-24.
15.    Ragavee A, Choedar T, Suman S, Devi SA. Evaluation of Bioactive Constituents and Antioxidant Activity of Mucilage Isolated from Tinospora cordifolia. Research Journal of Pharmacy and Technology. 2018; 11(7): 2747-2751.
16.    Buranrat B, Mairuae N, Kanchanarach W. Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells. Biomedical Reports. 2017; 6(4): 441-448.
17.    Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 2011; 1(1): 103-104.
18.    Banu KS, Cathrine L. General Techniques Involved in Phytochemical Analysis. International Journal of Advanced Research in Chemical Science. 2015; 2(4): 25-32.
19.    Kaur R, Kumar N. Phytochemical Composition and in vitro Antioxidant Activity of Leucas aspera Leaves. Research Journal of Pharmacy and Technology. 2016; 9(12): 2217-2221.
20.    Aouam I, EL Atki Y, Taleb M, Taroq A, EL Kamari F, Lyoussi B, Abdellaoui A. Antioxidant capacities and total phenolic contents of Thymus riatarum. Materials Today: Proceedings. 2019; 13: 579-586.
21.    Samydurai P, Saradha M. Effects of Various Solvent on the Extraction of Antimicrobial, Antioxidant Phenolics from the Stem Bark of Decalepis hamiltonii Wight and Arn. Asian Journal of Research in Pharmaceutical Sciences. 2016; 6(2): 129-134.
22.    Mahmoudi S, Khali M, Benkhaled A, Benamirouche K, Baiti I. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine. 2016; 6(3): 239-245.
23.    Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. Asian Journal of Pharmaceutical Analysis. 2019; 9(2): 67-76.
24.    Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini MS, Conti GO, Sadrabad EK. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food and Chemical Toxicology. 2018; 114: 108-111.
25.    El-sayed MM, El-hashash MM, Mohamed HR, Abdel-lateef EE. Phytochemical Investigation and in vitro Antioxidant Activity of Different Leaf Extracts of Salix mucronata Thunb. Journal of Applied Pharmaceutical Science. 2015; 5(12): 80-85.
26.    Lalthanpuii PB, Lalawmpuii R, Lalchhandama K. Study on the phytochemical constituents and some biological activities of the toothache plant Acmella oleracea, cultivated in Mizoram, India. Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(3): 152-155.
27.    Uddin K, Juraimi AS, Ali E, Ismail MR. Evaluation of Antioxidant Properties and Mineral Composition of Purslane (Portulaca oleracea L.) at Different Growth Stages. International Journal of Molecular Sciences. 2012; 13: 10257-10267.
28.    Scherer R, Godoy H. Antioxidant activity index (AAI) by the 2, 2- diphenyl-1-picrylhydrazyl method. Food Chemistry. 2009; 112: 654-658.
29.    Olugbami JO, Gbadegesin MA, Odunola OA. In vitro free radical scavenging and antioxidant properties of ethanol extract of Terminalia glaucescens. Pharmacognosy Research. 2014; 7(1): 49-56.
30.    Wang M, Li C, Li H, Wu Z, Chen B, Lei Y, Shen Y. In Vitro and In Silico Antioxidant Activity of Novel Peptides Prepared from Paeonia Ostii ‘Feng Dan’ Hydrolysate. Antioxidants. 2019; 8: 433-447.
31.    Patel DS, Shah PB, Managoli NB. Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian Journal of Pharmacy and Technology. 2012; 2(4): 143-147.
32.    Taukoorah U, Mahomoodally MF. Crude Aloe vera Gel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose Movement In Vitro. Advances in Pharmacological Sciences. 2016; 2016: 3720850.
33.    Chitra V, Tamilanban T, Manasa K, Narayanan J. Evaluation of In-vitro Antioxidant Activity of Ethanolic Extract of Ugli Fruit. Research Journal of Pharmacy and Technology. 2017; 10(11): 3826-3830.
34.    Eftimová Z, Eftimová J, Balážová Ľ. Antioxidant Activity of Tokaj Essence. Potravinarstvo Slovak Journal of Food Sciences. 2018; 12(1): 323-329.  
35.    Skehan P, Storeng R, Scudiero D, Monks A, Mcmahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of the National Cancer Institute. 1990; 82(13): 1107-1112.
36.    Conforti F, Marrelli M, Statti G, Menichini F. Antioxidant and cytotoxic activities of methanolic extract and fractions from Senecio gibbosus subsp. gibbosus (GUSS) DC. Natural Product Research. 2006; 20(9): 805-812.
37.    Prabha SB, Rao M, Kumar MRR. Evaluation of in vitro Antioxidant, Antibacterial and Anticancer activities of leaf extracts of Cleome rutidosperma. Research Journal of Pharmacy and Technology. 2017; 10(8): 2492-2496.
38.    Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim, AB. Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicology Letters. 2018; 291: 77-85.
39.    Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010; 2(12): 1231-1246.
40.    Merghem M, Dahamna S, Khennouf S. In Vivo Antioxidant Activity of Ruta montana L. Extracts. Journal of Materials and Environmental Science. 2019; 10(5): 470-477.
41.    Lalhminghlui K, Jagetia GC. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Science OA. 2018; 4(2): FSO272.
42.    Sajjadi SE, Ghanadian M, Haghighi M, Mouhebat L. Cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells. Journal of Herbmed Pharmacology. 2015; 4(1): 15-19.

Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): Peethala Prathyusha, Raja Sundararajan, Palyam Bhanu, Mathrusri Annapurna Mukthinuthalapati

DOI: 10.5958/0974-360X.2020.00507.7         Access: Open Access Read More

Author(s): Vijayalakshmi. S , Disalva. X, Chittaranjan Srivastava, Arun A

DOI: 10.5958/0974-360X.2019.00520.1         Access: Open Access Read More

Author(s): Yarnykh T. G., Kotvitska A. A., Tykhonov A. I., Rukhmakova O. A.

DOI: 10.5958/0974-360X.2020.00614.9         Access: Open Access Read More

Author(s): R. P. Bhole, S. R. Jagtap, K. B. Chadar, Y. B. Zambare

DOI: 10.5958/0974-360X.2020.00189.4         Access: Open Access Read More

Author(s): Suha Alnaeb, Youssef Alahmad, Saleh Trefi

DOI: 10.5958/0974-360X.2019.01035.7         Access: Open Access Read More

Author(s): Dhara Parekh, Pankaj Kapupara, Ketan Shah

DOI: dharaparekh92@gmail.com         Access: Open Access Read More

Author(s): R Deveswaran, S Bharath, BV Basavaraj, Sindhu Abraham, Sharon Furtado, V Madhavan

DOI:         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Gopinath G, Thirumal M, P. R. Kumar

DOI: 10.5958/0974-360X.2020.00362.5         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Bahubali N. Patil, Vamshi Krishna Tippavajhala, Girish Pai K

DOI: 10.5958/0974-360X.2019.00140.9         Access: Open Access Read More

Author(s): Mythili. L, GNK. Ganesh, C. Monisha, Kayalvizhi. R

DOI: 10.5958/0974-360X.2019.00426.8         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Prabhakar Panzade, Prashant K Puranik

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Suchithra. A. B, S. Jeganath, E. Jeevitha

DOI: 10.5958/0974-360X.2019.01073.4         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags